Experiment: Bas10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliA and fliZ are separated by 45 nucleotides fliZ and tcyJ are separated by 30 nucleotides tcyJ and dcyD are separated by 104 nucleotides
JDDGAC_00370: fliA - RNA polymerase sigma factor FliA, at 65,176 to 65,895
fliA
JDDGAC_00375: fliZ - flagella biosynthesis regulatory protein FliZ, at 65,941 to 66,492
fliZ
JDDGAC_00380: tcyJ - cystine ABC transporter substrate-binding protein, at 66,523 to 67,380
tcyJ
JDDGAC_00385: dcyD - D-cysteine desulfhydrase, at 67,485 to 68,471
dcyD
Position (kb) 65
66
67 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 65.072 kb on + strand at 65.084 kb on - strand at 65.155 kb on + strand at 65.156 kb on - strand at 65.157 kb on + strand at 65.157 kb on + strand at 65.158 kb on - strand at 65.189 kb on - strand at 65.310 kb on - strand, within fliA at 65.350 kb on + strand, within fliA at 65.377 kb on - strand, within fliA at 65.377 kb on - strand, within fliA at 65.377 kb on - strand, within fliA at 65.384 kb on - strand, within fliA at 65.384 kb on - strand, within fliA at 65.487 kb on - strand, within fliA at 65.535 kb on - strand, within fliA at 65.693 kb on + strand, within fliA at 65.693 kb on + strand, within fliA at 65.694 kb on - strand, within fliA at 65.762 kb on + strand, within fliA at 65.765 kb on + strand, within fliA at 65.777 kb on - strand, within fliA at 65.846 kb on + strand at 65.877 kb on + strand at 65.891 kb on + strand at 65.894 kb on - strand at 65.916 kb on + strand at 65.927 kb on - strand at 65.939 kb on + strand at 65.940 kb on - strand at 66.050 kb on + strand, within fliZ at 66.174 kb on - strand, within fliZ at 66.268 kb on + strand, within fliZ at 66.269 kb on - strand, within fliZ at 66.269 kb on - strand, within fliZ at 66.395 kb on - strand, within fliZ at 66.483 kb on + strand at 66.485 kb on + strand at 66.486 kb on - strand at 66.487 kb on + strand at 66.487 kb on + strand at 66.507 kb on + strand at 66.560 kb on + strand at 66.561 kb on - strand at 66.561 kb on - strand at 66.587 kb on + strand at 66.588 kb on - strand at 66.726 kb on + strand, within tcyJ at 66.830 kb on + strand, within tcyJ at 66.852 kb on + strand, within tcyJ at 66.874 kb on - strand, within tcyJ at 66.879 kb on + strand, within tcyJ at 66.880 kb on - strand, within tcyJ at 67.003 kb on - strand, within tcyJ at 67.003 kb on - strand, within tcyJ at 67.057 kb on + strand, within tcyJ at 67.101 kb on + strand, within tcyJ at 67.101 kb on + strand, within tcyJ at 67.105 kb on + strand, within tcyJ at 67.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas10 remove 65,072 + -0.3 65,084 - -2.0 65,155 + -0.3 65,156 - -1.4 65,157 + +0.5 65,157 + +0.4 65,158 - +1.5 65,189 - -1.0 65,310 - fliA JDDGAC_00370 0.19 +0.1 65,350 + fliA JDDGAC_00370 0.24 -1.3 65,377 - fliA JDDGAC_00370 0.28 -0.9 65,377 - fliA JDDGAC_00370 0.28 +0.0 65,377 - fliA JDDGAC_00370 0.28 -0.6 65,384 - fliA JDDGAC_00370 0.29 -2.1 65,384 - fliA JDDGAC_00370 0.29 +0.3 65,487 - fliA JDDGAC_00370 0.43 +0.4 65,535 - fliA JDDGAC_00370 0.50 +0.8 65,693 + fliA JDDGAC_00370 0.72 +0.9 65,693 + fliA JDDGAC_00370 0.72 -1.5 65,694 - fliA JDDGAC_00370 0.72 +0.1 65,762 + fliA JDDGAC_00370 0.81 -0.3 65,765 + fliA JDDGAC_00370 0.82 +0.1 65,777 - fliA JDDGAC_00370 0.83 +0.9 65,846 + -1.6 65,877 + +1.7 65,891 + +1.5 65,894 - +0.3 65,916 + +0.6 65,927 - -0.2 65,939 + -0.6 65,940 - +0.4 66,050 + fliZ JDDGAC_00375 0.20 +0.6 66,174 - fliZ JDDGAC_00375 0.42 +0.3 66,268 + fliZ JDDGAC_00375 0.59 +0.3 66,269 - fliZ JDDGAC_00375 0.59 +0.6 66,269 - fliZ JDDGAC_00375 0.59 -1.5 66,395 - fliZ JDDGAC_00375 0.82 -0.2 66,483 + -0.1 66,485 + +0.6 66,486 - +0.1 66,487 + +0.7 66,487 + +1.9 66,507 + +0.1 66,560 + +0.8 66,561 - -0.1 66,561 - +0.2 66,587 + +1.6 66,588 - +3.6 66,726 + tcyJ JDDGAC_00380 0.24 -0.4 66,830 + tcyJ JDDGAC_00380 0.36 -1.0 66,852 + tcyJ JDDGAC_00380 0.38 -1.6 66,874 - tcyJ JDDGAC_00380 0.41 +0.4 66,879 + tcyJ JDDGAC_00380 0.41 -0.4 66,880 - tcyJ JDDGAC_00380 0.42 +0.6 67,003 - tcyJ JDDGAC_00380 0.56 +0.6 67,003 - tcyJ JDDGAC_00380 0.56 -0.6 67,057 + tcyJ JDDGAC_00380 0.62 +1.2 67,101 + tcyJ JDDGAC_00380 0.67 +0.6 67,101 + tcyJ JDDGAC_00380 0.67 -0.1 67,105 + tcyJ JDDGAC_00380 0.68 -1.1 67,472 - +0.6
Or see this region's nucleotide sequence