Strain Fitness in Escherichia coli ECRC101 around MCAODC_22500

Experiment: GB_Bas36

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_22490 and MCAODC_22495 overlap by 76 nucleotidesMCAODC_22495 and espN are separated by 168 nucleotidesespN and nleL are separated by 590 nucleotides MCAODC_22490: MCAODC_22490 - IS3 family transposase, at 1,256,195 to 1,257,100 _22490 MCAODC_22495: MCAODC_22495 - CP4-44 prophage putative disrupted hemin or colicin receptor, at 1,257,025 to 1,257,474 _22495 MCAODC_22500: espN - type III secretion system effector EspN, at 1,257,643 to 1,261,044 espN MCAODC_22505: nleL - type III secretion system effector E3 ubiquitin ligase NleL, at 1,261,635 to 1,263,983 nleL Position (kb) 1257 1258 1259 1260 1261 1262Strain fitness (log2 ratio) -3 -2 -1 0 1at 1256.890 kb on - strand, within MCAODC_22490at 1257.064 kb on + strandat 1257.407 kb on - strand, within MCAODC_22495at 1257.500 kb on - strandat 1257.510 kb on + strandat 1257.553 kb on - strandat 1257.628 kb on + strandat 1257.628 kb on + strandat 1258.136 kb on - strand, within espNat 1258.198 kb on + strand, within espNat 1258.206 kb on + strand, within espNat 1258.789 kb on + strand, within espNat 1258.808 kb on + strand, within espNat 1260.482 kb on - strand, within espNat 1260.661 kb on - strand, within espN

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_Bas36
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1,256,890 - MCAODC_22490 0.77 +1.6
1,257,064 + -2.7
1,257,407 - MCAODC_22495 0.85 +0.5
1,257,500 - +0.2
1,257,510 + -1.6
1,257,553 - -0.5
1,257,628 + -1.7
1,257,628 + -2.0
1,258,136 - espN MCAODC_22500 0.14 -0.3
1,258,198 + espN MCAODC_22500 0.16 +1.6
1,258,206 + espN MCAODC_22500 0.17 +1.1
1,258,789 + espN MCAODC_22500 0.34 -2.2
1,258,808 + espN MCAODC_22500 0.34 -3.2
1,260,482 - espN MCAODC_22500 0.83 -0.9
1,260,661 - espN MCAODC_22500 0.89 -0.2

Or see this region's nucleotide sequence