Experiment: GB_Bas36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_22490 and MCAODC_22495 overlap by 76 nucleotides MCAODC_22495 and espN are separated by 168 nucleotides espN and nleL are separated by 590 nucleotides
MCAODC_22490: MCAODC_22490 - IS3 family transposase, at 1,256,195 to 1,257,100
_22490
MCAODC_22495: MCAODC_22495 - CP4-44 prophage putative disrupted hemin or colicin receptor, at 1,257,025 to 1,257,474
_22495
MCAODC_22500: espN - type III secretion system effector EspN, at 1,257,643 to 1,261,044
espN
MCAODC_22505: nleL - type III secretion system effector E3 ubiquitin ligase NleL, at 1,261,635 to 1,263,983
nleL
Position (kb)
1257
1258
1259
1260
1261
1262 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1256.890 kb on - strand, within MCAODC_22490 at 1257.064 kb on + strand at 1257.407 kb on - strand, within MCAODC_22495 at 1257.500 kb on - strand at 1257.510 kb on + strand at 1257.553 kb on - strand at 1257.628 kb on + strand at 1257.628 kb on + strand at 1258.136 kb on - strand, within espN at 1258.198 kb on + strand, within espN at 1258.206 kb on + strand, within espN at 1258.789 kb on + strand, within espN at 1258.808 kb on + strand, within espN at 1260.482 kb on - strand, within espN at 1260.661 kb on - strand, within espN
Per-strain Table
Position Strand Gene LocusTag Fraction GB_Bas36 remove 1,256,890 - MCAODC_22490 0.77 +1.6 1,257,064 + -2.7 1,257,407 - MCAODC_22495 0.85 +0.5 1,257,500 - +0.2 1,257,510 + -1.6 1,257,553 - -0.5 1,257,628 + -1.7 1,257,628 + -2.0 1,258,136 - espN MCAODC_22500 0.14 -0.3 1,258,198 + espN MCAODC_22500 0.16 +1.6 1,258,206 + espN MCAODC_22500 0.17 +1.1 1,258,789 + espN MCAODC_22500 0.34 -2.2 1,258,808 + espN MCAODC_22500 0.34 -3.2 1,260,482 - espN MCAODC_22500 0.83 -0.9 1,260,661 - espN MCAODC_22500 0.89 -0.2
Or see this region's nucleotide sequence