Strain Fitness in Escherichia coli ECRC101 around MCAODC_21775

Experiment: GB_Bas36

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntchaA and ldrB are separated by 398 nucleotidesldrB and MCAODC_21785 are separated by 428 nucleotidesMCAODC_21785 and kdsA are separated by 147 nucleotides MCAODC_21765: chaA - sodium-potassium/proton antiporter ChaA, at 1,138,663 to 1,139,763 chaA MCAODC_21775: ldrB - Small toxic polypeptide LdrB, at 1,140,162 to 1,140,269 ldrB MCAODC_21785: MCAODC_21785 - small toxic polypeptide LdrA/LdrC, at 1,140,698 to 1,140,805 _21785 MCAODC_21790: kdsA - 3-deoxy-8-phosphooctulonate synthase, at 1,140,953 to 1,141,807 kdsA Position (kb) 1140 1141Strain fitness (log2 ratio) -1 0 1 2at 1139.206 kb on + strand, within chaAat 1139.207 kb on - strand, within chaAat 1139.480 kb on + strand, within chaAat 1139.480 kb on + strand, within chaAat 1139.481 kb on - strand, within chaAat 1139.698 kb on - strandat 1139.740 kb on + strandat 1139.768 kb on + strandat 1139.768 kb on + strandat 1139.768 kb on + strandat 1139.768 kb on + strandat 1139.770 kb on + strandat 1139.771 kb on - strandat 1139.801 kb on + strandat 1139.802 kb on - strandat 1139.805 kb on - strandat 1139.807 kb on - strandat 1139.818 kb on + strandat 1139.836 kb on + strandat 1139.837 kb on - strandat 1140.228 kb on - strand, within ldrBat 1140.228 kb on - strand, within ldrBat 1140.304 kb on + strandat 1140.372 kb on - strandat 1140.372 kb on - strandat 1140.426 kb on + strandat 1140.682 kb on - strandat 1140.682 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_Bas36
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1,139,206 + chaA MCAODC_21765 0.49 -0.1
1,139,207 - chaA MCAODC_21765 0.49 +1.8
1,139,480 + chaA MCAODC_21765 0.74 -0.2
1,139,480 + chaA MCAODC_21765 0.74 -0.3
1,139,481 - chaA MCAODC_21765 0.74 +0.8
1,139,698 - +2.5
1,139,740 + +0.7
1,139,768 + +1.6
1,139,768 + -0.1
1,139,768 + -0.5
1,139,768 + +1.3
1,139,770 + -0.1
1,139,771 - +1.0
1,139,801 + +1.0
1,139,802 - +0.5
1,139,805 - +2.4
1,139,807 - -1.4
1,139,818 + +2.1
1,139,836 + -0.8
1,139,837 - -0.2
1,140,228 - ldrB MCAODC_21775 0.61 -1.6
1,140,228 - ldrB MCAODC_21775 0.61 -0.0
1,140,304 + -1.2
1,140,372 - -0.8
1,140,372 - -0.5
1,140,426 + +0.5
1,140,682 - +2.5
1,140,682 - +0.3

Or see this region's nucleotide sequence