Strain Fitness in Escherichia coli ECRC101 around MCAODC_20860

Experiment: GB_Bas36

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_20850 and MCAODC_20855 are separated by 4 nucleotidesMCAODC_20855 and MCAODC_20860 overlap by 4 nucleotidesMCAODC_20860 and MCAODC_20865 are separated by 66 nucleotidesMCAODC_20865 and MCAODC_20870 overlap by 8 nucleotidesMCAODC_20870 and lysC overlap by 38 nucleotides MCAODC_20850: MCAODC_20850 - Baseplate protein J, at 981,281 to 982,189 _20850 MCAODC_20855: MCAODC_20855 - baseplate assembly protein, at 982,194 to 982,541 _20855 MCAODC_20860: MCAODC_20860 - Spike protein, at 982,538 to 983,173 _20860 MCAODC_20865: MCAODC_20865 - phage virion morphogenesis protein, at 983,240 to 983,692 _20865 MCAODC_20870: MCAODC_20870 - Tail completion protein R, at 983,685 to 984,152 _20870 MCAODC_20875: lysC - Rz1-like lysis system protein LysC, at 984,115 to 984,273 lysC Position (kb) 982 983 984Strain fitness (log2 ratio) -2 -1 0 1 2at 981.539 kb on + strand, within MCAODC_20850at 981.659 kb on - strand, within MCAODC_20850at 981.682 kb on + strand, within MCAODC_20850at 981.754 kb on - strand, within MCAODC_20850at 981.785 kb on + strand, within MCAODC_20850at 981.860 kb on - strand, within MCAODC_20850at 981.903 kb on + strand, within MCAODC_20850at 981.904 kb on - strand, within MCAODC_20850at 981.904 kb on - strand, within MCAODC_20850at 981.991 kb on + strand, within MCAODC_20850at 982.003 kb on - strand, within MCAODC_20850at 982.340 kb on - strand, within MCAODC_20855at 982.395 kb on + strand, within MCAODC_20855at 982.449 kb on - strand, within MCAODC_20855at 982.449 kb on - strand, within MCAODC_20855at 982.449 kb on - strand, within MCAODC_20855at 982.478 kb on + strand, within MCAODC_20855at 982.478 kb on + strand, within MCAODC_20855at 982.595 kb on + strandat 982.596 kb on - strandat 982.596 kb on - strandat 982.600 kb on + strandat 982.801 kb on + strand, within MCAODC_20860at 982.839 kb on + strand, within MCAODC_20860at 982.917 kb on + strand, within MCAODC_20860at 983.241 kb on + strandat 983.242 kb on - strandat 983.242 kb on - strandat 983.263 kb on + strandat 983.322 kb on + strand, within MCAODC_20865at 983.462 kb on - strand, within MCAODC_20865at 983.465 kb on - strand, within MCAODC_20865at 983.720 kb on + strandat 983.721 kb on - strandat 983.721 kb on - strandat 983.771 kb on + strand, within MCAODC_20870at 983.844 kb on - strand, within MCAODC_20870at 984.010 kb on + strand, within MCAODC_20870at 984.054 kb on - strand, within MCAODC_20870at 984.054 kb on - strand, within MCAODC_20870at 984.072 kb on + strand, within MCAODC_20870at 984.084 kb on - strand, within MCAODC_20870

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_Bas36
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981,539 + MCAODC_20850 0.28 +0.4
981,659 - MCAODC_20850 0.42 +1.3
981,682 + MCAODC_20850 0.44 +1.7
981,754 - MCAODC_20850 0.52 -0.2
981,785 + MCAODC_20850 0.55 +0.2
981,860 - MCAODC_20850 0.64 -1.5
981,903 + MCAODC_20850 0.68 -0.3
981,904 - MCAODC_20850 0.69 +1.5
981,904 - MCAODC_20850 0.69 +0.8
981,991 + MCAODC_20850 0.78 +0.1
982,003 - MCAODC_20850 0.79 -0.5
982,340 - MCAODC_20855 0.42 -0.7
982,395 + MCAODC_20855 0.58 +0.5
982,449 - MCAODC_20855 0.73 -0.9
982,449 - MCAODC_20855 0.73 +0.1
982,449 - MCAODC_20855 0.73 -0.2
982,478 + MCAODC_20855 0.82 +2.1
982,478 + MCAODC_20855 0.82 +0.0
982,595 + +2.2
982,596 - +0.6
982,596 - +1.3
982,600 + -0.3
982,801 + MCAODC_20860 0.41 -1.7
982,839 + MCAODC_20860 0.47 -1.7
982,917 + MCAODC_20860 0.60 -1.1
983,241 + -0.1
983,242 - +0.6
983,242 - +0.5
983,263 + +0.4
983,322 + MCAODC_20865 0.18 +0.7
983,462 - MCAODC_20865 0.49 -0.2
983,465 - MCAODC_20865 0.50 +1.4
983,720 + +1.3
983,721 - +0.1
983,721 - -1.1
983,771 + MCAODC_20870 0.18 +0.8
983,844 - MCAODC_20870 0.34 +0.9
984,010 + MCAODC_20870 0.69 -1.5
984,054 - MCAODC_20870 0.79 -1.1
984,054 - MCAODC_20870 0.79 -0.1
984,072 + MCAODC_20870 0.83 -2.1
984,084 - MCAODC_20870 0.85 +0.3

Or see this region's nucleotide sequence