Strain Fitness in Escherichia coli ECRC101 around MCAODC_19035

Experiment: GB_Bas36

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntptrB and yebE are separated by 208 nucleotidesyebE and MCAODC_19035 are separated by 59 nucleotidesMCAODC_19035 and MCAODC_19040 are separated by 36 nucleotidesMCAODC_19040 and yebF overlap by 87 nucleotidesyebF and yebG are separated by 66 nucleotides MCAODC_19025: ptrB - oligopeptidase B, at 657,877 to 659,937 ptrB MCAODC_19030: yebE - Inner membrane protein YebE, at 660,146 to 660,805 yebE MCAODC_19035: MCAODC_19035 - hypothetical protein, at 660,865 to 660,975 _19035 MCAODC_19040: MCAODC_19040 - hypothetical protein, at 661,012 to 661,218 _19040 MCAODC_19045: yebF - Protein YebF, at 661,132 to 661,488 yebF MCAODC_19050: yebG - DNA damage-inducible protein YebG, at 661,555 to 661,845 yebG Position (kb) 660 661Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 659.941 kb on - strandat 659.966 kb on + strandat 659.967 kb on - strandat 659.967 kb on - strandat 659.967 kb on - strandat 659.968 kb on + strandat 659.969 kb on - strandat 659.969 kb on - strandat 659.969 kb on - strandat 659.969 kb on - strandat 660.244 kb on - strand, within yebEat 660.269 kb on - strand, within yebEat 660.294 kb on + strand, within yebEat 660.451 kb on + strand, within yebEat 660.451 kb on + strand, within yebEat 660.521 kb on - strand, within yebEat 660.577 kb on - strand, within yebEat 660.577 kb on - strand, within yebEat 660.639 kb on + strand, within yebEat 660.668 kb on - strand, within yebEat 660.668 kb on - strand, within yebEat 660.806 kb on - strandat 660.806 kb on - strandat 660.806 kb on - strandat 660.806 kb on - strandat 660.806 kb on - strandat 660.838 kb on + strandat 660.839 kb on - strandat 660.868 kb on + strandat 660.869 kb on - strandat 660.941 kb on + strand, within MCAODC_19035at 660.942 kb on - strand, within MCAODC_19035at 660.945 kb on + strand, within MCAODC_19035at 660.946 kb on - strand, within MCAODC_19035at 660.950 kb on + strand, within MCAODC_19035at 660.951 kb on - strand, within MCAODC_19035at 660.953 kb on - strand, within MCAODC_19035at 660.953 kb on - strand, within MCAODC_19035at 660.968 kb on + strandat 661.210 kb on + strandat 661.211 kb on - strandat 661.211 kb on - strandat 661.234 kb on - strand, within yebFat 661.528 kb on + strandat 661.764 kb on - strand, within yebGat 661.823 kb on - strandat 661.823 kb on - strandat 661.827 kb on - strandat 661.827 kb on - strandat 661.827 kb on - strandat 661.827 kb on - strandat 661.881 kb on + strandat 661.918 kb on - strandat 661.960 kb on + strandat 661.971 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_Bas36
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659,941 - -1.1
659,966 + +0.4
659,967 - +0.1
659,967 - +0.2
659,967 - +0.9
659,968 + +0.3
659,969 - +2.2
659,969 - -1.9
659,969 - -0.8
659,969 - +1.3
660,244 - yebE MCAODC_19030 0.15 +0.2
660,269 - yebE MCAODC_19030 0.19 +2.7
660,294 + yebE MCAODC_19030 0.22 -1.3
660,451 + yebE MCAODC_19030 0.46 -0.5
660,451 + yebE MCAODC_19030 0.46 -1.1
660,521 - yebE MCAODC_19030 0.57 -0.1
660,577 - yebE MCAODC_19030 0.65 +0.1
660,577 - yebE MCAODC_19030 0.65 +1.6
660,639 + yebE MCAODC_19030 0.75 +1.8
660,668 - yebE MCAODC_19030 0.79 +1.0
660,668 - yebE MCAODC_19030 0.79 -0.5
660,806 - -0.6
660,806 - -0.5
660,806 - +0.8
660,806 - +0.1
660,806 - +1.1
660,838 + -1.8
660,839 - +1.4
660,868 + -0.4
660,869 - +0.2
660,941 + MCAODC_19035 0.68 +4.4
660,942 - MCAODC_19035 0.69 -2.1
660,945 + MCAODC_19035 0.72 -2.5
660,946 - MCAODC_19035 0.73 -1.9
660,950 + MCAODC_19035 0.77 -0.8
660,951 - MCAODC_19035 0.77 -1.8
660,953 - MCAODC_19035 0.79 +0.8
660,953 - MCAODC_19035 0.79 +0.1
660,968 + -0.6
661,210 + -1.9
661,211 - +1.2
661,211 - +1.8
661,234 - yebF MCAODC_19045 0.29 -1.0
661,528 + +0.4
661,764 - yebG MCAODC_19050 0.72 -0.4
661,823 - +0.4
661,823 - -1.4
661,827 - -0.8
661,827 - -0.3
661,827 - +0.4
661,827 - +0.5
661,881 + -0.9
661,918 - +1.8
661,960 + -0.2
661,971 + -0.6

Or see this region's nucleotide sequence