Experiment: GB_Bas36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrF and yciH overlap by 1 nucleotides yciH and osmB are separated by 125 nucleotides osmB and yciT are separated by 268 nucleotides yciT and yciZ are separated by 89 nucleotides yciZ and MCAODC_15740 are separated by 147 nucleotides MCAODC_15740 and pdeR overlap by 14 nucleotides
MCAODC_15715: pyrF - orotidine-5'-phosphate decarboxylase, at 32,554 to 33,351
pyrF
MCAODC_15720: yciH - stress response translation initiation inhibitor YciH, at 33,351 to 33,677
yciH
MCAODC_15725: osmB - osmotically-inducible lipoprotein OsmB, at 33,803 to 34,021
osmB
MCAODC_15730: yciT - DNA-binding transcriptional regulator YciT, at 34,290 to 35,039
yciT
MCAODC_15735: yciZ - UPF0509 protein YciZ, at 35,129 to 35,302
yciZ
MCAODC_15740: MCAODC_15740 - EAL domain-containing protein, at 35,450 to 35,866
_15740
MCAODC_15745: pdeR - cyclic di-GMP phosphodiesterase, at 35,853 to 37,430
pdeR
Position (kb) 34
35
36 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 33.335 kb on + strand at 33.335 kb on + strand at 33.482 kb on + strand, within yciH at 33.852 kb on - strand, within osmB at 34.022 kb on - strand at 34.066 kb on + strand at 34.070 kb on + strand at 34.071 kb on - strand at 34.130 kb on + strand at 34.644 kb on - strand, within yciT at 34.831 kb on + strand, within yciT at 34.832 kb on - strand, within yciT at 35.063 kb on - strand at 35.080 kb on + strand at 35.130 kb on + strand at 35.198 kb on - strand, within yciZ at 35.229 kb on + strand, within yciZ at 35.408 kb on + strand at 35.411 kb on - strand at 35.438 kb on - strand at 35.439 kb on + strand at 35.440 kb on - strand at 35.476 kb on - strand at 35.579 kb on + strand, within MCAODC_15740 at 35.668 kb on + strand, within MCAODC_15740 at 35.668 kb on + strand, within MCAODC_15740 at 35.777 kb on + strand, within MCAODC_15740 at 35.857 kb on + strand at 35.991 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction GB_Bas36 remove 33,335 + +0.4 33,335 + -2.0 33,482 + yciH MCAODC_15720 0.40 -2.3 33,852 - osmB MCAODC_15725 0.22 +1.0 34,022 - -1.1 34,066 + -0.1 34,070 + +0.3 34,071 - +1.2 34,130 + -1.0 34,644 - yciT MCAODC_15730 0.47 +1.2 34,831 + yciT MCAODC_15730 0.72 -2.8 34,832 - yciT MCAODC_15730 0.72 -0.4 35,063 - +1.0 35,080 + +1.8 35,130 + +2.6 35,198 - yciZ MCAODC_15735 0.40 -1.5 35,229 + yciZ MCAODC_15735 0.57 +0.6 35,408 + +2.1 35,411 - -1.0 35,438 - +0.2 35,439 + -1.0 35,440 - +0.4 35,476 - +0.4 35,579 + MCAODC_15740 0.31 -2.8 35,668 + MCAODC_15740 0.52 -2.9 35,668 + MCAODC_15740 0.52 -0.0 35,777 + MCAODC_15740 0.78 -1.1 35,857 + -0.6 35,991 + +1.1
Or see this region's nucleotide sequence