Strain Fitness in Escherichia coli ECRC101 around MCAODC_15990

Experiment: Control_ECRC101

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_15980 and wcaG are separated by 290 nucleotideswcaG and MCAODC_15990 are separated by 20 nucleotidesMCAODC_15990 and MCAODC_15995 overlap by 62 nucleotidesMCAODC_15995 and ycjY overlap by 1 nucleotides MCAODC_15980: MCAODC_15980 - Murein peptide amidase A, at 87,672 to 88,400 _15980 MCAODC_15985: wcaG - NAD-dependent dehydratase, at 88,691 to 89,329 wcaG MCAODC_15990: MCAODC_15990 - Hydrolase, at 89,350 to 89,973 _15990 MCAODC_15995: MCAODC_15995 - Peptidase-S15 domain-containing protein, at 89,912 to 90,280 _15995 MCAODC_16000: ycjY - Uncharacterized protein YcjY, at 90,280 to 91,200 ycjY Position (kb) 89 90Strain fitness (log2 ratio) -3 -2 -1 0 1at 88.426 kb on - strandat 88.561 kb on + strandat 88.565 kb on + strandat 88.566 kb on - strandat 88.603 kb on + strandat 88.635 kb on - strandat 88.660 kb on - strandat 88.739 kb on + strandat 88.783 kb on + strand, within wcaGat 88.804 kb on + strand, within wcaGat 88.805 kb on - strand, within wcaGat 88.811 kb on + strand, within wcaGat 88.847 kb on - strand, within wcaGat 88.855 kb on - strand, within wcaGat 89.011 kb on - strand, within wcaGat 89.044 kb on + strand, within wcaGat 89.089 kb on + strand, within wcaGat 89.118 kb on + strand, within wcaGat 89.126 kb on - strand, within wcaGat 89.183 kb on - strand, within wcaGat 89.183 kb on - strand, within wcaGat 89.194 kb on + strand, within wcaGat 89.238 kb on - strand, within wcaGat 89.238 kb on - strand, within wcaGat 89.418 kb on - strand, within MCAODC_15990at 89.540 kb on - strand, within MCAODC_15990at 89.665 kb on - strand, within MCAODC_15990at 89.823 kb on - strand, within MCAODC_15990at 89.860 kb on - strand, within MCAODC_15990at 89.868 kb on + strand, within MCAODC_15990at 89.871 kb on + strand, within MCAODC_15990at 89.887 kb on - strand, within MCAODC_15990at 89.974 kb on - strand, within MCAODC_15995at 90.011 kb on - strand, within MCAODC_15995at 90.084 kb on + strand, within MCAODC_15995at 90.085 kb on - strand, within MCAODC_15995at 90.085 kb on - strand, within MCAODC_15995at 90.151 kb on + strand, within MCAODC_15995at 90.151 kb on + strand, within MCAODC_15995at 90.152 kb on - strand, within MCAODC_15995at 90.179 kb on - strand, within MCAODC_15995at 90.308 kb on - strandat 90.348 kb on - strandat 90.414 kb on - strand, within ycjYat 90.496 kb on - strand, within ycjYat 90.510 kb on - strand, within ycjYat 90.510 kb on - strand, within ycjYat 90.514 kb on - strand, within ycjYat 90.514 kb on - strand, within ycjYat 90.651 kb on + strand, within ycjYat 90.673 kb on - strand, within ycjYat 90.676 kb on - strand, within ycjYat 90.834 kb on + strand, within ycjYat 90.834 kb on + strand, within ycjYat 90.834 kb on + strand, within ycjYat 90.835 kb on - strand, within ycjYat 90.835 kb on - strand, within ycjYat 90.888 kb on - strand, within ycjY

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101
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88,426 - +0.3
88,561 + +0.5
88,565 + +0.3
88,566 - +0.8
88,603 + -1.7
88,635 - -0.3
88,660 - +0.0
88,739 + -0.2
88,783 + wcaG MCAODC_15985 0.14 -0.3
88,804 + wcaG MCAODC_15985 0.18 -0.6
88,805 - wcaG MCAODC_15985 0.18 +1.1
88,811 + wcaG MCAODC_15985 0.19 -0.6
88,847 - wcaG MCAODC_15985 0.24 +0.9
88,855 - wcaG MCAODC_15985 0.26 +0.2
89,011 - wcaG MCAODC_15985 0.50 +0.6
89,044 + wcaG MCAODC_15985 0.55 -0.0
89,089 + wcaG MCAODC_15985 0.62 +0.2
89,118 + wcaG MCAODC_15985 0.67 +0.4
89,126 - wcaG MCAODC_15985 0.68 -0.5
89,183 - wcaG MCAODC_15985 0.77 +1.2
89,183 - wcaG MCAODC_15985 0.77 +0.5
89,194 + wcaG MCAODC_15985 0.79 -0.6
89,238 - wcaG MCAODC_15985 0.86 +0.7
89,238 - wcaG MCAODC_15985 0.86 +0.6
89,418 - MCAODC_15990 0.11 +0.6
89,540 - MCAODC_15990 0.30 -2.2
89,665 - MCAODC_15990 0.50 +0.4
89,823 - MCAODC_15990 0.76 -0.0
89,860 - MCAODC_15990 0.82 -3.2
89,868 + MCAODC_15990 0.83 -2.1
89,871 + MCAODC_15990 0.83 +0.5
89,887 - MCAODC_15990 0.86 -1.5
89,974 - MCAODC_15995 0.17 +0.7
90,011 - MCAODC_15995 0.27 -0.3
90,084 + MCAODC_15995 0.47 -0.0
90,085 - MCAODC_15995 0.47 -0.4
90,085 - MCAODC_15995 0.47 +0.3
90,151 + MCAODC_15995 0.65 -0.9
90,151 + MCAODC_15995 0.65 -0.7
90,152 - MCAODC_15995 0.65 -1.7
90,179 - MCAODC_15995 0.72 -0.7
90,308 - +0.9
90,348 - +0.5
90,414 - ycjY MCAODC_16000 0.15 -0.5
90,496 - ycjY MCAODC_16000 0.23 -0.2
90,510 - ycjY MCAODC_16000 0.25 +0.1
90,510 - ycjY MCAODC_16000 0.25 -1.7
90,514 - ycjY MCAODC_16000 0.25 +0.7
90,514 - ycjY MCAODC_16000 0.25 -0.1
90,651 + ycjY MCAODC_16000 0.40 -0.1
90,673 - ycjY MCAODC_16000 0.43 -0.8
90,676 - ycjY MCAODC_16000 0.43 +0.3
90,834 + ycjY MCAODC_16000 0.60 -0.6
90,834 + ycjY MCAODC_16000 0.60 -0.2
90,834 + ycjY MCAODC_16000 0.60 -0.1
90,835 - ycjY MCAODC_16000 0.60 +0.9
90,835 - ycjY MCAODC_16000 0.60 +0.7
90,888 - ycjY MCAODC_16000 0.66 -1.1

Or see this region's nucleotide sequence