Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinG and espX2 are separated by 283 nucleotides espX2 and rhlE are separated by 607 nucleotides
MCAODC_25495: dinG - ATP-dependent DNA helicase DinG, at 1,821,043 to 1,823,193
dinG
MCAODC_25500: espX2 - type III secretion system effector EspX2, at 1,823,477 to 1,825,489
espX2
MCAODC_25505: rhlE - ATP-dependent RNA helicase RhlE, at 1,826,097 to 1,827,464
rhlE
Position (kb)
1823
1824
1825
1826 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1822.592 kb on + strand, within dinG at 1822.592 kb on + strand, within dinG at 1822.593 kb on - strand, within dinG at 1822.595 kb on + strand, within dinG at 1822.633 kb on + strand, within dinG at 1822.633 kb on + strand, within dinG at 1822.657 kb on + strand, within dinG at 1822.657 kb on + strand, within dinG at 1822.658 kb on - strand, within dinG at 1822.658 kb on - strand, within dinG at 1822.658 kb on - strand, within dinG at 1822.660 kb on - strand, within dinG at 1822.695 kb on + strand, within dinG at 1822.696 kb on - strand, within dinG at 1822.696 kb on - strand, within dinG at 1822.697 kb on + strand, within dinG at 1822.697 kb on + strand, within dinG at 1822.697 kb on + strand, within dinG at 1822.697 kb on + strand, within dinG at 1822.697 kb on + strand, within dinG at 1822.698 kb on - strand, within dinG at 1822.749 kb on + strand, within dinG at 1822.750 kb on - strand, within dinG at 1822.828 kb on - strand, within dinG at 1822.828 kb on - strand, within dinG at 1822.841 kb on + strand, within dinG at 1822.917 kb on - strand, within dinG at 1823.148 kb on + strand at 1823.150 kb on + strand at 1823.151 kb on - strand at 1823.215 kb on + strand at 1823.216 kb on - strand at 1823.307 kb on + strand at 1823.386 kb on + strand at 1823.555 kb on + strand at 1823.556 kb on - strand at 1823.632 kb on + strand at 1824.603 kb on - strand, within espX2 at 1824.946 kb on + strand, within espX2 at 1825.662 kb on + strand at 1825.694 kb on + strand at 1825.711 kb on + strand at 1825.770 kb on - strand at 1825.868 kb on + strand at 1826.088 kb on + strand at 1826.089 kb on - strand at 1826.089 kb on - strand at 1826.089 kb on - strand at 1826.095 kb on + strand at 1826.095 kb on + strand at 1826.096 kb on - strand at 1826.096 kb on - strand at 1826.096 kb on - strand at 1826.098 kb on + strand at 1826.099 kb on - strand at 1826.099 kb on - strand at 1826.479 kb on + strand, within rhlE at 1826.479 kb on + strand, within rhlE at 1826.479 kb on + strand, within rhlE at 1826.480 kb on - strand, within rhlE at 1826.480 kb on - strand, within rhlE at 1826.480 kb on - strand, within rhlE at 1826.480 kb on - strand, within rhlE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 1,822,592 + dinG MCAODC_25495 0.72 -0.2 1,822,592 + dinG MCAODC_25495 0.72 -0.4 1,822,593 - dinG MCAODC_25495 0.72 +0.3 1,822,595 + dinG MCAODC_25495 0.72 -1.1 1,822,633 + dinG MCAODC_25495 0.74 +1.0 1,822,633 + dinG MCAODC_25495 0.74 +0.6 1,822,657 + dinG MCAODC_25495 0.75 -1.1 1,822,657 + dinG MCAODC_25495 0.75 +1.2 1,822,658 - dinG MCAODC_25495 0.75 -0.8 1,822,658 - dinG MCAODC_25495 0.75 +0.5 1,822,658 - dinG MCAODC_25495 0.75 -3.1 1,822,660 - dinG MCAODC_25495 0.75 -0.9 1,822,695 + dinG MCAODC_25495 0.77 -0.1 1,822,696 - dinG MCAODC_25495 0.77 -0.2 1,822,696 - dinG MCAODC_25495 0.77 +0.9 1,822,697 + dinG MCAODC_25495 0.77 +0.5 1,822,697 + dinG MCAODC_25495 0.77 +1.2 1,822,697 + dinG MCAODC_25495 0.77 -2.9 1,822,697 + dinG MCAODC_25495 0.77 -2.0 1,822,697 + dinG MCAODC_25495 0.77 +0.7 1,822,698 - dinG MCAODC_25495 0.77 -2.1 1,822,749 + dinG MCAODC_25495 0.79 -0.6 1,822,750 - dinG MCAODC_25495 0.79 +1.4 1,822,828 - dinG MCAODC_25495 0.83 +2.4 1,822,828 - dinG MCAODC_25495 0.83 +1.5 1,822,841 + dinG MCAODC_25495 0.84 +0.1 1,822,917 - dinG MCAODC_25495 0.87 +0.2 1,823,148 + -0.7 1,823,150 + -0.3 1,823,151 - -1.6 1,823,215 + -0.4 1,823,216 - -1.0 1,823,307 + +0.0 1,823,386 + +0.3 1,823,555 + -0.6 1,823,556 - +0.7 1,823,632 + -0.9 1,824,603 - espX2 MCAODC_25500 0.56 -2.8 1,824,946 + espX2 MCAODC_25500 0.73 -0.6 1,825,662 + +0.1 1,825,694 + +1.6 1,825,711 + +0.5 1,825,770 - +1.0 1,825,868 + +1.0 1,826,088 + +0.2 1,826,089 - +0.1 1,826,089 - -0.0 1,826,089 - -1.1 1,826,095 + +0.3 1,826,095 + -1.0 1,826,096 - -0.6 1,826,096 - +1.3 1,826,096 - +0.4 1,826,098 + -2.0 1,826,099 - -0.1 1,826,099 - +0.1 1,826,479 + rhlE MCAODC_25505 0.28 +0.4 1,826,479 + rhlE MCAODC_25505 0.28 +0.3 1,826,479 + rhlE MCAODC_25505 0.28 -0.1 1,826,480 - rhlE MCAODC_25505 0.28 -0.1 1,826,480 - rhlE MCAODC_25505 0.28 -0.3 1,826,480 - rhlE MCAODC_25505 0.28 -0.7 1,826,480 - rhlE MCAODC_25505 0.28 -0.5
Or see this region's nucleotide sequence