Strain Fitness in Escherichia coli ECRC101 around MCAODC_23180

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclsC and ymdB overlap by 59 nucleotidesymdB and MCAODC_23180 are separated by 94 nucleotidesMCAODC_23180 and csgC are separated by 120 nucleotidescsgC and csgA are separated by 58 nucleotides MCAODC_23170: clsC - cardiolipin synthase ClsC, at 1,373,561 to 1,375,042 clsC MCAODC_23175: ymdB - O-acetyl-ADP-ribose deacetylase, at 1,374,984 to 1,375,517 ymdB MCAODC_23180: MCAODC_23180 - Uncharacterized protein YmdA, at 1,375,612 to 1,375,923 _23180 MCAODC_23185: csgC - curli assembly protein CsgC, at 1,376,044 to 1,376,376 csgC MCAODC_23190: csgA - curlin major subunit CsgA, at 1,376,435 to 1,376,893 csgA Position (kb) 1375 1376Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1374.628 kb on + strand, within clsCat 1374.629 kb on - strand, within clsCat 1374.630 kb on + strand, within clsCat 1374.631 kb on - strand, within clsCat 1374.631 kb on - strand, within clsCat 1374.631 kb on - strand, within clsCat 1374.835 kb on - strand, within clsCat 1374.853 kb on + strand, within clsCat 1375.054 kb on + strand, within ymdBat 1375.054 kb on + strand, within ymdBat 1375.054 kb on + strand, within ymdBat 1375.054 kb on + strand, within ymdBat 1375.054 kb on + strand, within ymdBat 1375.054 kb on + strand, within ymdBat 1375.055 kb on - strand, within ymdBat 1375.055 kb on - strand, within ymdBat 1375.055 kb on - strand, within ymdBat 1375.055 kb on - strand, within ymdBat 1375.055 kb on - strand, within ymdBat 1375.056 kb on + strand, within ymdBat 1375.057 kb on - strand, within ymdBat 1375.057 kb on - strand, within ymdBat 1375.085 kb on + strand, within ymdBat 1375.178 kb on + strand, within ymdBat 1375.178 kb on + strand, within ymdBat 1375.178 kb on + strand, within ymdBat 1375.178 kb on + strand, within ymdBat 1375.181 kb on - strand, within ymdBat 1375.216 kb on + strand, within ymdBat 1375.216 kb on + strand, within ymdBat 1375.435 kb on - strand, within ymdBat 1375.528 kb on + strandat 1375.528 kb on + strandat 1375.545 kb on + strandat 1375.613 kb on + strandat 1375.613 kb on + strandat 1375.614 kb on - strandat 1375.618 kb on - strandat 1375.619 kb on + strandat 1375.634 kb on - strandat 1375.662 kb on - strand, within MCAODC_23180at 1375.672 kb on + strand, within MCAODC_23180at 1375.682 kb on - strand, within MCAODC_23180at 1375.682 kb on - strand, within MCAODC_23180at 1375.682 kb on - strand, within MCAODC_23180at 1375.823 kb on + strand, within MCAODC_23180at 1375.870 kb on - strand, within MCAODC_23180at 1375.925 kb on + strandat 1375.931 kb on - strandat 1375.946 kb on - strandat 1375.973 kb on + strandat 1375.973 kb on + strandat 1376.023 kb on + strandat 1376.024 kb on - strandat 1376.110 kb on - strand, within csgCat 1376.142 kb on + strand, within csgCat 1376.254 kb on + strand, within csgCat 1376.296 kb on - strand, within csgCat 1376.303 kb on + strand, within csgCat 1376.303 kb on + strand, within csgCat 1376.303 kb on + strand, within csgCat 1376.303 kb on + strand, within csgCat 1376.303 kb on + strand, within csgCat 1376.303 kb on + strand, within csgCat 1376.303 kb on + strand, within csgCat 1376.325 kb on + strand, within csgCat 1376.329 kb on - strand, within csgCat 1376.329 kb on - strand, within csgCat 1376.363 kb on - strandat 1376.366 kb on - strandat 1376.433 kb on + strandat 1376.434 kb on - strandat 1376.434 kb on - strandat 1376.437 kb on - strandat 1376.437 kb on - strandat 1376.498 kb on - strand, within csgAat 1376.546 kb on - strand, within csgAat 1376.546 kb on - strand, within csgAat 1376.555 kb on - strand, within csgAat 1376.555 kb on - strand, within csgAat 1376.566 kb on + strand, within csgAat 1376.684 kb on - strand, within csgAat 1376.687 kb on - strand, within csgAat 1376.687 kb on - strand, within csgAat 1376.743 kb on - strand, within csgAat 1376.749 kb on - strand, within csgAat 1376.752 kb on + strand, within csgA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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1,374,628 + clsC MCAODC_23170 0.72 +1.4
1,374,629 - clsC MCAODC_23170 0.72 +4.0
1,374,630 + clsC MCAODC_23170 0.72 -0.2
1,374,631 - clsC MCAODC_23170 0.72 -1.5
1,374,631 - clsC MCAODC_23170 0.72 -0.1
1,374,631 - clsC MCAODC_23170 0.72 -1.7
1,374,835 - clsC MCAODC_23170 0.86 +0.9
1,374,853 + clsC MCAODC_23170 0.87 -1.8
1,375,054 + ymdB MCAODC_23175 0.13 -2.0
1,375,054 + ymdB MCAODC_23175 0.13 -0.0
1,375,054 + ymdB MCAODC_23175 0.13 +1.6
1,375,054 + ymdB MCAODC_23175 0.13 +0.5
1,375,054 + ymdB MCAODC_23175 0.13 -2.6
1,375,054 + ymdB MCAODC_23175 0.13 -1.3
1,375,055 - ymdB MCAODC_23175 0.13 +0.7
1,375,055 - ymdB MCAODC_23175 0.13 +0.8
1,375,055 - ymdB MCAODC_23175 0.13 -0.9
1,375,055 - ymdB MCAODC_23175 0.13 +0.5
1,375,055 - ymdB MCAODC_23175 0.13 -0.8
1,375,056 + ymdB MCAODC_23175 0.13 +1.4
1,375,057 - ymdB MCAODC_23175 0.14 -0.8
1,375,057 - ymdB MCAODC_23175 0.14 +0.4
1,375,085 + ymdB MCAODC_23175 0.19 +0.4
1,375,178 + ymdB MCAODC_23175 0.36 -0.4
1,375,178 + ymdB MCAODC_23175 0.36 +0.9
1,375,178 + ymdB MCAODC_23175 0.36 +2.2
1,375,178 + ymdB MCAODC_23175 0.36 +0.6
1,375,181 - ymdB MCAODC_23175 0.37 -1.8
1,375,216 + ymdB MCAODC_23175 0.43 +0.1
1,375,216 + ymdB MCAODC_23175 0.43 -0.8
1,375,435 - ymdB MCAODC_23175 0.84 -1.2
1,375,528 + +1.2
1,375,528 + +0.4
1,375,545 + +0.4
1,375,613 + -2.6
1,375,613 + -2.5
1,375,614 - +0.5
1,375,618 - +0.5
1,375,619 + +1.6
1,375,634 - -0.4
1,375,662 - MCAODC_23180 0.16 -1.3
1,375,672 + MCAODC_23180 0.19 +0.3
1,375,682 - MCAODC_23180 0.22 -2.0
1,375,682 - MCAODC_23180 0.22 -2.4
1,375,682 - MCAODC_23180 0.22 -2.9
1,375,823 + MCAODC_23180 0.68 -1.0
1,375,870 - MCAODC_23180 0.83 +1.4
1,375,925 + -1.8
1,375,931 - +0.7
1,375,946 - +0.3
1,375,973 + +0.5
1,375,973 + -0.8
1,376,023 + -0.8
1,376,024 - -1.7
1,376,110 - csgC MCAODC_23185 0.20 -1.0
1,376,142 + csgC MCAODC_23185 0.29 +0.4
1,376,254 + csgC MCAODC_23185 0.63 -2.6
1,376,296 - csgC MCAODC_23185 0.76 -1.7
1,376,303 + csgC MCAODC_23185 0.78 +1.1
1,376,303 + csgC MCAODC_23185 0.78 +0.5
1,376,303 + csgC MCAODC_23185 0.78 +0.8
1,376,303 + csgC MCAODC_23185 0.78 +0.3
1,376,303 + csgC MCAODC_23185 0.78 +0.4
1,376,303 + csgC MCAODC_23185 0.78 -1.8
1,376,303 + csgC MCAODC_23185 0.78 +1.2
1,376,325 + csgC MCAODC_23185 0.84 +0.1
1,376,329 - csgC MCAODC_23185 0.86 -1.7
1,376,329 - csgC MCAODC_23185 0.86 +0.1
1,376,363 - +0.4
1,376,366 - -0.4
1,376,433 + -0.4
1,376,434 - -0.5
1,376,434 - -0.9
1,376,437 - +1.0
1,376,437 - -0.4
1,376,498 - csgA MCAODC_23190 0.14 -1.7
1,376,546 - csgA MCAODC_23190 0.24 -0.7
1,376,546 - csgA MCAODC_23190 0.24 -0.9
1,376,555 - csgA MCAODC_23190 0.26 -1.6
1,376,555 - csgA MCAODC_23190 0.26 -1.2
1,376,566 + csgA MCAODC_23190 0.29 -1.4
1,376,684 - csgA MCAODC_23190 0.54 +0.5
1,376,687 - csgA MCAODC_23190 0.55 -0.5
1,376,687 - csgA MCAODC_23190 0.55 +0.7
1,376,743 - csgA MCAODC_23190 0.67 -1.4
1,376,749 - csgA MCAODC_23190 0.68 +1.6
1,376,752 + csgA MCAODC_23190 0.69 -0.4

Or see this region's nucleotide sequence