Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_17155 and MCAODC_17160 overlap by 8 nucleotides MCAODC_17160 and MCAODC_17165 overlap by 4 nucleotides MCAODC_17165 and MCAODC_17170 are separated by 32 nucleotides MCAODC_17170 and MCAODC_17175 are separated by 33 nucleotides MCAODC_17175 and MCAODC_17180 overlap by 8 nucleotides
MCAODC_17155: MCAODC_17155 - Prophage protein, at 326,274 to 326,585
_17155
MCAODC_17160: MCAODC_17160 - hypothetical protein, at 326,578 to 326,805
_17160
MCAODC_17165: MCAODC_17165 - DNA-binding protein, at 326,802 to 327,083
_17165
MCAODC_17170: MCAODC_17170 - DUF1627 domain-containing protein, at 327,116 to 327,832
_17170
MCAODC_17175: MCAODC_17175 - replication protein, at 327,866 to 328,327
_17175
MCAODC_17180: MCAODC_17180 - phage replisome organizer, at 328,320 to 329,363
_17180
Position (kb) 327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 326.246 kb on + strand at 326.388 kb on - strand, within MCAODC_17155 at 326.444 kb on - strand, within MCAODC_17155 at 326.613 kb on - strand, within MCAODC_17160 at 326.642 kb on + strand, within MCAODC_17160 at 326.643 kb on - strand, within MCAODC_17160 at 326.643 kb on - strand, within MCAODC_17160 at 326.643 kb on - strand, within MCAODC_17160 at 326.645 kb on - strand, within MCAODC_17160 at 326.784 kb on - strand at 326.793 kb on - strand at 326.833 kb on + strand, within MCAODC_17165 at 326.845 kb on - strand, within MCAODC_17165 at 326.845 kb on - strand, within MCAODC_17165 at 326.967 kb on + strand, within MCAODC_17165 at 326.967 kb on + strand, within MCAODC_17165 at 326.968 kb on - strand, within MCAODC_17165 at 326.968 kb on - strand, within MCAODC_17165 at 327.048 kb on + strand, within MCAODC_17165 at 327.049 kb on - strand, within MCAODC_17165 at 327.049 kb on - strand, within MCAODC_17165 at 327.078 kb on + strand at 327.078 kb on + strand at 327.078 kb on + strand at 327.146 kb on - strand at 327.554 kb on - strand, within MCAODC_17170 at 327.898 kb on - strand at 328.127 kb on - strand, within MCAODC_17175 at 328.127 kb on - strand, within MCAODC_17175 at 328.127 kb on - strand, within MCAODC_17175 at 328.746 kb on - strand, within MCAODC_17180
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 326,246 + +1.6 326,388 - MCAODC_17155 0.37 +0.5 326,444 - MCAODC_17155 0.54 -0.3 326,613 - MCAODC_17160 0.15 -3.3 326,642 + MCAODC_17160 0.28 +0.5 326,643 - MCAODC_17160 0.29 +2.1 326,643 - MCAODC_17160 0.29 -0.3 326,643 - MCAODC_17160 0.29 +0.1 326,645 - MCAODC_17160 0.29 -1.2 326,784 - -1.2 326,793 - +0.9 326,833 + MCAODC_17165 0.11 +0.0 326,845 - MCAODC_17165 0.15 -1.2 326,845 - MCAODC_17165 0.15 -3.6 326,967 + MCAODC_17165 0.59 -0.6 326,967 + MCAODC_17165 0.59 -3.4 326,968 - MCAODC_17165 0.59 -1.7 326,968 - MCAODC_17165 0.59 -0.2 327,048 + MCAODC_17165 0.87 +0.5 327,049 - MCAODC_17165 0.88 +0.1 327,049 - MCAODC_17165 0.88 -0.1 327,078 + +2.4 327,078 + +0.7 327,078 + -2.0 327,146 - +0.5 327,554 - MCAODC_17170 0.61 -1.9 327,898 - -1.1 328,127 - MCAODC_17175 0.56 -1.1 328,127 - MCAODC_17175 0.56 -1.9 328,127 - MCAODC_17175 0.56 -0.5 328,746 - MCAODC_17180 0.41 -1.8
Or see this region's nucleotide sequence