Strain Fitness in Escherichia coli ECRC101 around MCAODC_14895

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybcL and ybcM are separated by 9 nucleotidesybcM and ybcN are separated by 214 nucleotidesybcN and MCAODC_14905 overlap by 1 nucleotidesMCAODC_14905 and ybcO overlap by 8 nucleotides MCAODC_14890: ybcL - Raf kinase inhibitor-like protein YbcL, at 2,920,790 to 2,921,341 ybcL MCAODC_14895: ybcM - Uncharacterized HTH-type transcriptional regulator YbcM, at 2,921,351 to 2,922,148 ybcM MCAODC_14900: ybcN - DNA base-flipping protein YbcN, at 2,922,363 to 2,922,818 ybcN MCAODC_14905: MCAODC_14905 - Prophage protein NinE, at 2,922,818 to 2,922,988 _14905 MCAODC_14910: ybcO - Putative nuclease YbcO, at 2,922,981 to 2,923,271 ybcO Position (kb) 2921 2922 2923Strain fitness (log2 ratio) -3 -2 -1 0 1at 2921.546 kb on + strand, within ybcMat 2921.915 kb on + strand, within ybcMat 2922.543 kb on + strand, within ybcNat 2922.543 kb on + strand, within ybcNat 2922.587 kb on - strand, within ybcN

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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2,921,546 + ybcM MCAODC_14895 0.24 -3.6
2,921,915 + ybcM MCAODC_14895 0.71 +0.1
2,922,543 + ybcN MCAODC_14900 0.39 -0.6
2,922,543 + ybcN MCAODC_14900 0.39 -0.8
2,922,587 - ybcN MCAODC_14900 0.49 -0.3

Or see this region's nucleotide sequence