Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolB and tolA are separated by 132 nucleotides tolA and tolR are separated by 64 nucleotides tolR and tolQ are separated by 3 nucleotides
MCAODC_26025: tolB - Tol-Pal system protein TolB, at 1,917,250 to 1,918,542
tolB
MCAODC_26030: tolA - cell envelope integrity protein TolA, at 1,918,675 to 1,919,949
tolA
MCAODC_26035: tolR - colicin uptake protein TolR, at 1,920,014 to 1,920,442
tolR
MCAODC_26040: tolQ - Tol-Pal system protein TolQ, at 1,920,446 to 1,921,138
tolQ
Position (kb)
1918
1919
1920 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1917.679 kb on - strand, within tolB at 1917.731 kb on + strand, within tolB at 1917.732 kb on - strand, within tolB at 1917.836 kb on - strand, within tolB at 1917.897 kb on - strand, within tolB at 1917.906 kb on + strand, within tolB at 1917.974 kb on + strand, within tolB at 1917.983 kb on + strand, within tolB at 1918.037 kb on + strand, within tolB at 1918.065 kb on + strand, within tolB at 1918.069 kb on - strand, within tolB at 1918.115 kb on + strand, within tolB at 1918.160 kb on - strand, within tolB at 1918.209 kb on - strand, within tolB at 1918.286 kb on - strand, within tolB at 1918.344 kb on + strand, within tolB at 1918.505 kb on - strand at 1918.545 kb on + strand at 1918.546 kb on - strand at 1918.546 kb on - strand at 1918.546 kb on - strand at 1918.554 kb on + strand at 1918.600 kb on + strand at 1918.615 kb on + strand at 1918.718 kb on - strand at 1918.755 kb on + strand at 1918.756 kb on - strand at 1918.822 kb on - strand, within tolA at 1918.868 kb on + strand, within tolA at 1918.869 kb on - strand, within tolA at 1918.884 kb on - strand, within tolA at 1918.923 kb on - strand, within tolA at 1918.926 kb on + strand, within tolA at 1918.963 kb on - strand, within tolA at 1918.965 kb on + strand, within tolA at 1919.157 kb on + strand, within tolA at 1919.514 kb on + strand, within tolA at 1919.875 kb on + strand at 1919.902 kb on - strand at 1919.906 kb on - strand at 1920.015 kb on + strand at 1920.016 kb on - strand at 1920.016 kb on - strand at 1920.016 kb on - strand at 1920.019 kb on + strand at 1920.033 kb on - strand at 1920.058 kb on + strand, within tolR at 1920.062 kb on + strand, within tolR at 1920.084 kb on - strand, within tolR at 1920.523 kb on - strand, within tolQ at 1920.723 kb on + strand, within tolQ at 1920.888 kb on + strand, within tolQ at 1920.942 kb on + strand, within tolQ at 1920.943 kb on - strand, within tolQ
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 1,917,679 - tolB MCAODC_26025 0.33 +0.6 1,917,731 + tolB MCAODC_26025 0.37 -0.5 1,917,732 - tolB MCAODC_26025 0.37 -2.5 1,917,836 - tolB MCAODC_26025 0.45 -1.6 1,917,897 - tolB MCAODC_26025 0.50 +0.0 1,917,906 + tolB MCAODC_26025 0.51 -1.8 1,917,974 + tolB MCAODC_26025 0.56 -1.1 1,917,983 + tolB MCAODC_26025 0.57 +2.1 1,918,037 + tolB MCAODC_26025 0.61 -0.1 1,918,065 + tolB MCAODC_26025 0.63 -1.9 1,918,069 - tolB MCAODC_26025 0.63 -2.6 1,918,115 + tolB MCAODC_26025 0.67 -0.7 1,918,160 - tolB MCAODC_26025 0.70 -0.7 1,918,209 - tolB MCAODC_26025 0.74 -0.9 1,918,286 - tolB MCAODC_26025 0.80 -0.6 1,918,344 + tolB MCAODC_26025 0.85 -1.0 1,918,505 - -2.0 1,918,545 + -1.1 1,918,546 - -0.9 1,918,546 - +0.3 1,918,546 - -2.3 1,918,554 + -1.9 1,918,600 + +0.5 1,918,615 + -0.5 1,918,718 - -0.2 1,918,755 + +1.6 1,918,756 - +0.3 1,918,822 - tolA MCAODC_26030 0.12 -1.2 1,918,868 + tolA MCAODC_26030 0.15 -1.2 1,918,869 - tolA MCAODC_26030 0.15 -0.7 1,918,884 - tolA MCAODC_26030 0.16 +1.6 1,918,923 - tolA MCAODC_26030 0.19 +1.0 1,918,926 + tolA MCAODC_26030 0.20 -1.6 1,918,963 - tolA MCAODC_26030 0.23 -1.1 1,918,965 + tolA MCAODC_26030 0.23 -1.3 1,919,157 + tolA MCAODC_26030 0.38 -0.4 1,919,514 + tolA MCAODC_26030 0.66 +0.6 1,919,875 + +2.6 1,919,902 - -0.9 1,919,906 - +1.0 1,920,015 + +1.3 1,920,016 - -0.7 1,920,016 - -0.6 1,920,016 - -0.2 1,920,019 + -0.3 1,920,033 - -0.3 1,920,058 + tolR MCAODC_26035 0.10 +0.3 1,920,062 + tolR MCAODC_26035 0.11 +0.1 1,920,084 - tolR MCAODC_26035 0.16 -1.6 1,920,523 - tolQ MCAODC_26040 0.11 +2.6 1,920,723 + tolQ MCAODC_26040 0.40 +1.0 1,920,888 + tolQ MCAODC_26040 0.64 +1.6 1,920,942 + tolQ MCAODC_26040 0.72 -2.8 1,920,943 - tolQ MCAODC_26040 0.72 -1.1
Or see this region's nucleotide sequence