Strain Fitness in Escherichia coli ECRC101 around MCAODC_26030

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolB and tolA are separated by 132 nucleotidestolA and tolR are separated by 64 nucleotidestolR and tolQ are separated by 3 nucleotides MCAODC_26025: tolB - Tol-Pal system protein TolB, at 1,917,250 to 1,918,542 tolB MCAODC_26030: tolA - cell envelope integrity protein TolA, at 1,918,675 to 1,919,949 tolA MCAODC_26035: tolR - colicin uptake protein TolR, at 1,920,014 to 1,920,442 tolR MCAODC_26040: tolQ - Tol-Pal system protein TolQ, at 1,920,446 to 1,921,138 tolQ Position (kb) 1918 1919 1920Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1917.679 kb on - strand, within tolBat 1917.731 kb on + strand, within tolBat 1917.732 kb on - strand, within tolBat 1917.836 kb on - strand, within tolBat 1917.897 kb on - strand, within tolBat 1917.906 kb on + strand, within tolBat 1917.974 kb on + strand, within tolBat 1917.983 kb on + strand, within tolBat 1918.037 kb on + strand, within tolBat 1918.065 kb on + strand, within tolBat 1918.069 kb on - strand, within tolBat 1918.115 kb on + strand, within tolBat 1918.160 kb on - strand, within tolBat 1918.209 kb on - strand, within tolBat 1918.286 kb on - strand, within tolBat 1918.344 kb on + strand, within tolBat 1918.505 kb on - strandat 1918.545 kb on + strandat 1918.546 kb on - strandat 1918.546 kb on - strandat 1918.546 kb on - strandat 1918.554 kb on + strandat 1918.600 kb on + strandat 1918.615 kb on + strandat 1918.718 kb on - strandat 1918.755 kb on + strandat 1918.756 kb on - strandat 1918.822 kb on - strand, within tolAat 1918.868 kb on + strand, within tolAat 1918.869 kb on - strand, within tolAat 1918.884 kb on - strand, within tolAat 1918.923 kb on - strand, within tolAat 1918.926 kb on + strand, within tolAat 1918.963 kb on - strand, within tolAat 1918.965 kb on + strand, within tolAat 1919.157 kb on + strand, within tolAat 1919.514 kb on + strand, within tolAat 1919.875 kb on + strandat 1919.902 kb on - strandat 1919.906 kb on - strandat 1920.015 kb on + strandat 1920.016 kb on - strandat 1920.016 kb on - strandat 1920.016 kb on - strandat 1920.019 kb on + strandat 1920.033 kb on - strandat 1920.058 kb on + strand, within tolRat 1920.062 kb on + strand, within tolRat 1920.084 kb on - strand, within tolRat 1920.523 kb on - strand, within tolQat 1920.723 kb on + strand, within tolQat 1920.888 kb on + strand, within tolQat 1920.942 kb on + strand, within tolQat 1920.943 kb on - strand, within tolQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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1,917,679 - tolB MCAODC_26025 0.33 +0.6
1,917,731 + tolB MCAODC_26025 0.37 -0.5
1,917,732 - tolB MCAODC_26025 0.37 -2.5
1,917,836 - tolB MCAODC_26025 0.45 -1.6
1,917,897 - tolB MCAODC_26025 0.50 +0.0
1,917,906 + tolB MCAODC_26025 0.51 -1.8
1,917,974 + tolB MCAODC_26025 0.56 -1.1
1,917,983 + tolB MCAODC_26025 0.57 +2.1
1,918,037 + tolB MCAODC_26025 0.61 -0.1
1,918,065 + tolB MCAODC_26025 0.63 -1.9
1,918,069 - tolB MCAODC_26025 0.63 -2.6
1,918,115 + tolB MCAODC_26025 0.67 -0.7
1,918,160 - tolB MCAODC_26025 0.70 -0.7
1,918,209 - tolB MCAODC_26025 0.74 -0.9
1,918,286 - tolB MCAODC_26025 0.80 -0.6
1,918,344 + tolB MCAODC_26025 0.85 -1.0
1,918,505 - -2.0
1,918,545 + -1.1
1,918,546 - -0.9
1,918,546 - +0.3
1,918,546 - -2.3
1,918,554 + -1.9
1,918,600 + +0.5
1,918,615 + -0.5
1,918,718 - -0.2
1,918,755 + +1.6
1,918,756 - +0.3
1,918,822 - tolA MCAODC_26030 0.12 -1.2
1,918,868 + tolA MCAODC_26030 0.15 -1.2
1,918,869 - tolA MCAODC_26030 0.15 -0.7
1,918,884 - tolA MCAODC_26030 0.16 +1.6
1,918,923 - tolA MCAODC_26030 0.19 +1.0
1,918,926 + tolA MCAODC_26030 0.20 -1.6
1,918,963 - tolA MCAODC_26030 0.23 -1.1
1,918,965 + tolA MCAODC_26030 0.23 -1.3
1,919,157 + tolA MCAODC_26030 0.38 -0.4
1,919,514 + tolA MCAODC_26030 0.66 +0.6
1,919,875 + +2.6
1,919,902 - -0.9
1,919,906 - +1.0
1,920,015 + +1.3
1,920,016 - -0.7
1,920,016 - -0.6
1,920,016 - -0.2
1,920,019 + -0.3
1,920,033 - -0.3
1,920,058 + tolR MCAODC_26035 0.10 +0.3
1,920,062 + tolR MCAODC_26035 0.11 +0.1
1,920,084 - tolR MCAODC_26035 0.16 -1.6
1,920,523 - tolQ MCAODC_26040 0.11 +2.6
1,920,723 + tolQ MCAODC_26040 0.40 +1.0
1,920,888 + tolQ MCAODC_26040 0.64 +1.6
1,920,942 + tolQ MCAODC_26040 0.72 -2.8
1,920,943 - tolQ MCAODC_26040 0.72 -1.1

Or see this region's nucleotide sequence