Strain Fitness in Escherichia coli ECRC101 around MCAODC_20425

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisG and hisD are separated by 5 nucleotideshisD and hisC overlap by 4 nucleotides MCAODC_20420: hisG - ATP phosphoribosyltransferase, at 890,147 to 891,046 hisG MCAODC_20425: hisD - Histidinol dehydrogenase, at 891,052 to 892,356 hisD MCAODC_20430: hisC - histidinol-phosphate transaminase, at 892,353 to 893,423 hisC Position (kb) 891 892 893Strain fitness (log2 ratio) -3 -2 -1 0 1at 890.131 kb on - strandat 890.131 kb on - strandat 890.141 kb on - strandat 890.177 kb on - strandat 890.177 kb on - strandat 890.177 kb on - strandat 890.177 kb on - strandat 890.177 kb on - strandat 890.236 kb on + strandat 890.347 kb on - strand, within hisGat 890.423 kb on + strand, within hisGat 890.423 kb on + strand, within hisGat 890.423 kb on + strand, within hisGat 890.424 kb on - strand, within hisGat 890.424 kb on - strand, within hisGat 890.707 kb on + strand, within hisGat 890.707 kb on + strand, within hisGat 890.711 kb on + strand, within hisGat 890.712 kb on - strand, within hisGat 890.733 kb on + strand, within hisGat 890.828 kb on + strand, within hisGat 890.828 kb on + strand, within hisGat 890.828 kb on + strand, within hisGat 890.828 kb on + strand, within hisGat 891.081 kb on + strandat 891.081 kb on + strandat 891.082 kb on - strandat 891.113 kb on + strandat 891.213 kb on + strand, within hisDat 891.372 kb on - strand, within hisDat 891.377 kb on + strand, within hisDat 891.377 kb on + strand, within hisDat 891.377 kb on + strand, within hisDat 891.378 kb on - strand, within hisDat 891.439 kb on + strand, within hisDat 891.440 kb on - strand, within hisDat 891.440 kb on - strand, within hisDat 891.440 kb on - strand, within hisDat 891.476 kb on + strand, within hisDat 891.477 kb on - strand, within hisDat 891.479 kb on + strand, within hisDat 891.480 kb on - strand, within hisDat 891.502 kb on - strand, within hisDat 892.094 kb on + strand, within hisDat 892.094 kb on + strand, within hisDat 892.166 kb on - strand, within hisDat 892.320 kb on + strandat 892.321 kb on - strandat 892.342 kb on - strandat 892.367 kb on + strandat 892.367 kb on + strandat 892.378 kb on + strandat 892.468 kb on - strand, within hisCat 892.494 kb on - strand, within hisCat 892.560 kb on + strand, within hisCat 892.561 kb on - strand, within hisCat 892.614 kb on - strand, within hisCat 892.614 kb on - strand, within hisCat 892.680 kb on + strand, within hisCat 892.762 kb on + strand, within hisCat 892.763 kb on - strand, within hisCat 892.803 kb on + strand, within hisCat 892.804 kb on - strand, within hisCat 892.911 kb on + strand, within hisCat 892.912 kb on - strand, within hisCat 892.912 kb on - strand, within hisCat 892.912 kb on - strand, within hisCat 892.912 kb on - strand, within hisCat 892.912 kb on - strand, within hisCat 892.973 kb on + strand, within hisCat 893.027 kb on + strand, within hisCat 893.027 kb on + strand, within hisCat 893.028 kb on - strand, within hisCat 893.028 kb on - strand, within hisCat 893.168 kb on + strand, within hisCat 893.247 kb on + strand, within hisCat 893.248 kb on - strand, within hisCat 893.248 kb on - strand, within hisCat 893.310 kb on - strand, within hisC

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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890,131 - -0.6
890,131 - -2.4
890,141 - -0.8
890,177 - -0.8
890,177 - +0.4
890,177 - -2.4
890,177 - -1.5
890,177 - -0.8
890,236 + -2.0
890,347 - hisG MCAODC_20420 0.22 -1.2
890,423 + hisG MCAODC_20420 0.31 -0.9
890,423 + hisG MCAODC_20420 0.31 -1.1
890,423 + hisG MCAODC_20420 0.31 +0.4
890,424 - hisG MCAODC_20420 0.31 -2.3
890,424 - hisG MCAODC_20420 0.31 +0.3
890,707 + hisG MCAODC_20420 0.62 -0.2
890,707 + hisG MCAODC_20420 0.62 -0.3
890,711 + hisG MCAODC_20420 0.63 -0.2
890,712 - hisG MCAODC_20420 0.63 -3.3
890,733 + hisG MCAODC_20420 0.65 -0.9
890,828 + hisG MCAODC_20420 0.76 +0.5
890,828 + hisG MCAODC_20420 0.76 -0.3
890,828 + hisG MCAODC_20420 0.76 -2.1
890,828 + hisG MCAODC_20420 0.76 -0.9
891,081 + -0.9
891,081 + -1.1
891,082 - -2.0
891,113 + -1.5
891,213 + hisD MCAODC_20425 0.12 -1.4
891,372 - hisD MCAODC_20425 0.25 -3.5
891,377 + hisD MCAODC_20425 0.25 -1.1
891,377 + hisD MCAODC_20425 0.25 -0.4
891,377 + hisD MCAODC_20425 0.25 -0.4
891,378 - hisD MCAODC_20425 0.25 -2.1
891,439 + hisD MCAODC_20425 0.30 -2.8
891,440 - hisD MCAODC_20425 0.30 -0.4
891,440 - hisD MCAODC_20425 0.30 -0.4
891,440 - hisD MCAODC_20425 0.30 -0.4
891,476 + hisD MCAODC_20425 0.32 -1.2
891,477 - hisD MCAODC_20425 0.33 -0.3
891,479 + hisD MCAODC_20425 0.33 -3.2
891,480 - hisD MCAODC_20425 0.33 -1.8
891,502 - hisD MCAODC_20425 0.34 -0.4
892,094 + hisD MCAODC_20425 0.80 -0.9
892,094 + hisD MCAODC_20425 0.80 -0.8
892,166 - hisD MCAODC_20425 0.85 -1.9
892,320 + -0.4
892,321 - -2.8
892,342 - -0.8
892,367 + -0.7
892,367 + +0.3
892,378 + -2.7
892,468 - hisC MCAODC_20430 0.11 -0.8
892,494 - hisC MCAODC_20430 0.13 -0.4
892,560 + hisC MCAODC_20430 0.19 -1.8
892,561 - hisC MCAODC_20430 0.19 +0.8
892,614 - hisC MCAODC_20430 0.24 -0.8
892,614 - hisC MCAODC_20430 0.24 +1.3
892,680 + hisC MCAODC_20430 0.31 -0.9
892,762 + hisC MCAODC_20430 0.38 -1.2
892,763 - hisC MCAODC_20430 0.38 -2.3
892,803 + hisC MCAODC_20430 0.42 -1.8
892,804 - hisC MCAODC_20430 0.42 -1.7
892,911 + hisC MCAODC_20430 0.52 +0.2
892,912 - hisC MCAODC_20430 0.52 -1.3
892,912 - hisC MCAODC_20430 0.52 -1.7
892,912 - hisC MCAODC_20430 0.52 -2.3
892,912 - hisC MCAODC_20430 0.52 +1.0
892,912 - hisC MCAODC_20430 0.52 -1.4
892,973 + hisC MCAODC_20430 0.58 -2.5
893,027 + hisC MCAODC_20430 0.63 -1.5
893,027 + hisC MCAODC_20430 0.63 -0.2
893,028 - hisC MCAODC_20430 0.63 -2.4
893,028 - hisC MCAODC_20430 0.63 -1.1
893,168 + hisC MCAODC_20430 0.76 -1.2
893,247 + hisC MCAODC_20430 0.83 -0.7
893,248 - hisC MCAODC_20430 0.84 +0.2
893,248 - hisC MCAODC_20430 0.84 -0.3
893,310 - hisC MCAODC_20430 0.89 -0.3

Or see this region's nucleotide sequence