Strain Fitness in Escherichia coli ECRC101 around MCAODC_15590

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciF and yciG are separated by 85 nucleotidesyciG and trpA are separated by 380 nucleotidestrpA and trpB overlap by 1 nucleotides MCAODC_15580: yciF - Protein YciF, at 6,277 to 6,777 yciF MCAODC_15585: yciG - Uncharacterized protein YciG, at 6,863 to 7,042 yciG MCAODC_15590: trpA - tryptophan synthase subunit alpha, at 7,423 to 8,229 trpA MCAODC_15595: trpB - tryptophan synthase subunit beta, at 8,229 to 9,422 trpB Position (kb) 7 8 9Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 6.444 kb on - strand, within yciFat 6.444 kb on - strand, within yciFat 6.505 kb on - strand, within yciFat 6.523 kb on - strand, within yciFat 7.275 kb on + strandat 7.301 kb on + strandat 7.301 kb on + strandat 7.346 kb on + strandat 7.346 kb on + strandat 7.494 kb on + strandat 7.622 kb on + strand, within trpAat 7.733 kb on - strand, within trpAat 7.779 kb on + strand, within trpAat 7.779 kb on + strand, within trpAat 7.779 kb on + strand, within trpAat 7.792 kb on - strand, within trpAat 7.909 kb on - strand, within trpAat 7.925 kb on + strand, within trpAat 7.925 kb on + strand, within trpAat 7.926 kb on - strand, within trpAat 7.926 kb on - strand, within trpAat 8.081 kb on - strand, within trpAat 8.081 kb on - strand, within trpAat 8.310 kb on + strandat 8.453 kb on - strand, within trpBat 8.475 kb on - strand, within trpBat 8.531 kb on - strand, within trpBat 8.587 kb on + strand, within trpBat 8.587 kb on + strand, within trpBat 8.864 kb on + strand, within trpBat 8.865 kb on - strand, within trpBat 8.866 kb on + strand, within trpBat 8.866 kb on + strand, within trpBat 9.025 kb on + strand, within trpBat 9.025 kb on + strand, within trpBat 9.025 kb on + strand, within trpBat 9.025 kb on + strand, within trpBat 9.116 kb on + strand, within trpBat 9.135 kb on + strand, within trpB

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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6,444 - yciF MCAODC_15580 0.33 +1.9
6,444 - yciF MCAODC_15580 0.33 +0.8
6,505 - yciF MCAODC_15580 0.46 +3.7
6,523 - yciF MCAODC_15580 0.49 +0.0
7,275 + -1.0
7,301 + -0.1
7,301 + -0.4
7,346 + +0.5
7,346 + +2.2
7,494 + -3.9
7,622 + trpA MCAODC_15590 0.25 -3.3
7,733 - trpA MCAODC_15590 0.38 -4.7
7,779 + trpA MCAODC_15590 0.44 -0.6
7,779 + trpA MCAODC_15590 0.44 -1.7
7,779 + trpA MCAODC_15590 0.44 -0.6
7,792 - trpA MCAODC_15590 0.46 -0.9
7,909 - trpA MCAODC_15590 0.60 -2.9
7,925 + trpA MCAODC_15590 0.62 -3.7
7,925 + trpA MCAODC_15590 0.62 -2.1
7,926 - trpA MCAODC_15590 0.62 -1.2
7,926 - trpA MCAODC_15590 0.62 -2.7
8,081 - trpA MCAODC_15590 0.82 -2.5
8,081 - trpA MCAODC_15590 0.82 -0.8
8,310 + -3.2
8,453 - trpB MCAODC_15595 0.19 -0.3
8,475 - trpB MCAODC_15595 0.21 -3.4
8,531 - trpB MCAODC_15595 0.25 -2.9
8,587 + trpB MCAODC_15595 0.30 +0.1
8,587 + trpB MCAODC_15595 0.30 -0.9
8,864 + trpB MCAODC_15595 0.53 -3.8
8,865 - trpB MCAODC_15595 0.53 +0.2
8,866 + trpB MCAODC_15595 0.53 -1.7
8,866 + trpB MCAODC_15595 0.53 -2.2
9,025 + trpB MCAODC_15595 0.67 -3.0
9,025 + trpB MCAODC_15595 0.67 -2.2
9,025 + trpB MCAODC_15595 0.67 -1.8
9,025 + trpB MCAODC_15595 0.67 +0.7
9,116 + trpB MCAODC_15595 0.74 -1.3
9,135 + trpB MCAODC_15595 0.76 -0.8

Or see this region's nucleotide sequence