Strain Fitness in Escherichia coli ECRC101 around MCAODC_12720

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdxK and crr are separated by 42 nucleotidescrr and ptsI are separated by 40 nucleotidesptsI and ptsH are separated by 44 nucleotidesptsH and cysK are separated by 383 nucleotides MCAODC_12710: pdxK - pyridoxine/pyridoxal/pyridoxamine kinase, at 2,523,337 to 2,524,188 pdxK MCAODC_12715: crr - PTS glucose transporter subunit IIA, at 2,524,231 to 2,524,740 crr MCAODC_12720: ptsI - phosphoenolpyruvate-protein phosphotransferase PtsI, at 2,524,781 to 2,526,508 ptsI MCAODC_12725: ptsH - phosphocarrier protein Hpr, at 2,526,553 to 2,526,810 ptsH MCAODC_12730: cysK - cysteine synthase A, at 2,527,194 to 2,528,165 cysK Position (kb) 2524 2525 2526 2527Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2523.814 kb on - strand, within pdxKat 2523.903 kb on - strand, within pdxKat 2524.054 kb on - strand, within pdxKat 2524.054 kb on - strand, within pdxKat 2524.186 kb on + strandat 2524.826 kb on + strandat 2524.910 kb on - strandat 2525.045 kb on - strand, within ptsIat 2525.089 kb on - strand, within ptsIat 2525.284 kb on - strand, within ptsIat 2525.681 kb on - strand, within ptsIat 2526.120 kb on - strand, within ptsIat 2526.555 kb on - strandat 2526.555 kb on - strandat 2526.624 kb on + strand, within ptsHat 2526.882 kb on - strandat 2526.882 kb on - strandat 2526.928 kb on + strandat 2526.929 kb on - strandat 2526.929 kb on - strandat 2526.969 kb on - strandat 2526.977 kb on - strandat 2526.983 kb on + strandat 2526.983 kb on + strandat 2526.983 kb on + strandat 2526.984 kb on - strandat 2527.086 kb on + strandat 2527.245 kb on + strandat 2527.245 kb on + strandat 2527.246 kb on - strandat 2527.249 kb on + strandat 2527.352 kb on + strand, within cysKat 2527.353 kb on - strand, within cysKat 2527.364 kb on + strand, within cysKat 2527.365 kb on - strand, within cysKat 2527.365 kb on - strand, within cysKat 2527.454 kb on + strand, within cysKat 2527.478 kb on + strand, within cysKat 2527.506 kb on - strand, within cysK

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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2,523,814 - pdxK MCAODC_12710 0.56 -0.3
2,523,903 - pdxK MCAODC_12710 0.66 +1.1
2,524,054 - pdxK MCAODC_12710 0.84 -0.5
2,524,054 - pdxK MCAODC_12710 0.84 +0.9
2,524,186 + +1.8
2,524,826 + +2.7
2,524,910 - +0.3
2,525,045 - ptsI MCAODC_12720 0.15 -0.3
2,525,089 - ptsI MCAODC_12720 0.18 -0.5
2,525,284 - ptsI MCAODC_12720 0.29 +0.3
2,525,681 - ptsI MCAODC_12720 0.52 +0.1
2,526,120 - ptsI MCAODC_12720 0.77 -1.7
2,526,555 - -0.1
2,526,555 - -1.1
2,526,624 + ptsH MCAODC_12725 0.28 -0.5
2,526,882 - -2.4
2,526,882 - +0.3
2,526,928 + -0.3
2,526,929 - +0.1
2,526,929 - -0.0
2,526,969 - +1.0
2,526,977 - +1.4
2,526,983 + +2.2
2,526,983 + -1.7
2,526,983 + -0.9
2,526,984 - +0.3
2,527,086 + -0.4
2,527,245 + -0.5
2,527,245 + -0.5
2,527,246 - -1.5
2,527,249 + -1.4
2,527,352 + cysK MCAODC_12730 0.16 -2.5
2,527,353 - cysK MCAODC_12730 0.16 +0.4
2,527,364 + cysK MCAODC_12730 0.17 -2.8
2,527,365 - cysK MCAODC_12730 0.18 -2.3
2,527,365 - cysK MCAODC_12730 0.18 -0.4
2,527,454 + cysK MCAODC_12730 0.27 -1.2
2,527,478 + cysK MCAODC_12730 0.29 -1.9
2,527,506 - cysK MCAODC_12730 0.32 -1.5

Or see this region's nucleotide sequence