Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxK and crr are separated by 42 nucleotides crr and ptsI are separated by 40 nucleotides ptsI and ptsH are separated by 44 nucleotides ptsH and cysK are separated by 383 nucleotides
MCAODC_12710: pdxK - pyridoxine/pyridoxal/pyridoxamine kinase, at 2,523,337 to 2,524,188
pdxK
MCAODC_12715: crr - PTS glucose transporter subunit IIA, at 2,524,231 to 2,524,740
crr
MCAODC_12720: ptsI - phosphoenolpyruvate-protein phosphotransferase PtsI, at 2,524,781 to 2,526,508
ptsI
MCAODC_12725: ptsH - phosphocarrier protein Hpr, at 2,526,553 to 2,526,810
ptsH
MCAODC_12730: cysK - cysteine synthase A, at 2,527,194 to 2,528,165
cysK
Position (kb)
2524
2525
2526
2527 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2523.814 kb on - strand, within pdxK at 2523.903 kb on - strand, within pdxK at 2524.054 kb on - strand, within pdxK at 2524.054 kb on - strand, within pdxK at 2524.186 kb on + strand at 2524.826 kb on + strand at 2524.910 kb on - strand at 2525.045 kb on - strand, within ptsI at 2525.089 kb on - strand, within ptsI at 2525.284 kb on - strand, within ptsI at 2525.681 kb on - strand, within ptsI at 2526.120 kb on - strand, within ptsI at 2526.555 kb on - strand at 2526.555 kb on - strand at 2526.624 kb on + strand, within ptsH at 2526.882 kb on - strand at 2526.882 kb on - strand at 2526.928 kb on + strand at 2526.929 kb on - strand at 2526.929 kb on - strand at 2526.969 kb on - strand at 2526.977 kb on - strand at 2526.983 kb on + strand at 2526.983 kb on + strand at 2526.983 kb on + strand at 2526.984 kb on - strand at 2527.086 kb on + strand at 2527.245 kb on + strand at 2527.245 kb on + strand at 2527.246 kb on - strand at 2527.249 kb on + strand at 2527.352 kb on + strand, within cysK at 2527.353 kb on - strand, within cysK at 2527.364 kb on + strand, within cysK at 2527.365 kb on - strand, within cysK at 2527.365 kb on - strand, within cysK at 2527.454 kb on + strand, within cysK at 2527.478 kb on + strand, within cysK at 2527.506 kb on - strand, within cysK
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 2,523,814 - pdxK MCAODC_12710 0.56 -0.3 2,523,903 - pdxK MCAODC_12710 0.66 +1.1 2,524,054 - pdxK MCAODC_12710 0.84 -0.5 2,524,054 - pdxK MCAODC_12710 0.84 +0.9 2,524,186 + +1.8 2,524,826 + +2.7 2,524,910 - +0.3 2,525,045 - ptsI MCAODC_12720 0.15 -0.3 2,525,089 - ptsI MCAODC_12720 0.18 -0.5 2,525,284 - ptsI MCAODC_12720 0.29 +0.3 2,525,681 - ptsI MCAODC_12720 0.52 +0.1 2,526,120 - ptsI MCAODC_12720 0.77 -1.7 2,526,555 - -0.1 2,526,555 - -1.1 2,526,624 + ptsH MCAODC_12725 0.28 -0.5 2,526,882 - -2.4 2,526,882 - +0.3 2,526,928 + -0.3 2,526,929 - +0.1 2,526,929 - -0.0 2,526,969 - +1.0 2,526,977 - +1.4 2,526,983 + +2.2 2,526,983 + -1.7 2,526,983 + -0.9 2,526,984 - +0.3 2,527,086 + -0.4 2,527,245 + -0.5 2,527,245 + -0.5 2,527,246 - -1.5 2,527,249 + -1.4 2,527,352 + cysK MCAODC_12730 0.16 -2.5 2,527,353 - cysK MCAODC_12730 0.16 +0.4 2,527,364 + cysK MCAODC_12730 0.17 -2.8 2,527,365 - cysK MCAODC_12730 0.18 -2.3 2,527,365 - cysK MCAODC_12730 0.18 -0.4 2,527,454 + cysK MCAODC_12730 0.27 -1.2 2,527,478 + cysK MCAODC_12730 0.29 -1.9 2,527,506 - cysK MCAODC_12730 0.32 -1.5
Or see this region's nucleotide sequence