Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysI and cysH are separated by 73 nucleotides cysH and hokG are separated by 263 nucleotides hokG and cas3 are separated by 191 nucleotides
MCAODC_10900: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 2,174,580 to 2,176,292
cysI
MCAODC_10905: cysH - phosphoadenosine phosphosulfate reductase, at 2,176,366 to 2,177,100
cysH
MCAODC_10915: hokG - Putative protein HokG, at 2,177,364 to 2,177,516
hokG
MCAODC_10920: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 2,177,708 to 2,180,407
cas3
Position (kb)
2176
2177
2178 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2175.378 kb on + strand, within cysI at 2175.422 kb on + strand, within cysI at 2175.423 kb on - strand, within cysI at 2175.423 kb on - strand, within cysI at 2175.423 kb on - strand, within cysI at 2175.504 kb on + strand, within cysI at 2175.588 kb on + strand, within cysI at 2175.614 kb on + strand, within cysI at 2175.614 kb on + strand, within cysI at 2175.641 kb on + strand, within cysI at 2175.642 kb on - strand, within cysI at 2175.756 kb on - strand, within cysI at 2175.839 kb on - strand, within cysI at 2175.941 kb on + strand, within cysI at 2175.961 kb on + strand, within cysI at 2176.006 kb on + strand, within cysI at 2176.007 kb on - strand, within cysI at 2176.081 kb on - strand, within cysI at 2176.118 kb on + strand, within cysI at 2176.131 kb on + strand at 2176.132 kb on - strand at 2176.291 kb on - strand at 2176.319 kb on + strand at 2176.320 kb on - strand at 2176.365 kb on + strand at 2176.366 kb on - strand at 2176.366 kb on - strand at 2176.382 kb on + strand at 2176.383 kb on - strand at 2176.409 kb on + strand at 2176.410 kb on - strand at 2176.476 kb on - strand, within cysH at 2176.507 kb on + strand, within cysH at 2176.507 kb on + strand, within cysH at 2176.507 kb on + strand, within cysH at 2176.507 kb on + strand, within cysH at 2176.508 kb on - strand, within cysH at 2176.512 kb on - strand, within cysH at 2176.578 kb on + strand, within cysH at 2176.632 kb on + strand, within cysH at 2176.675 kb on + strand, within cysH at 2176.756 kb on - strand, within cysH at 2176.892 kb on + strand, within cysH at 2176.896 kb on + strand, within cysH at 2176.897 kb on - strand, within cysH at 2176.897 kb on - strand, within cysH at 2176.935 kb on - strand, within cysH at 2176.942 kb on + strand, within cysH at 2176.967 kb on - strand, within cysH at 2177.013 kb on + strand, within cysH at 2177.117 kb on + strand at 2177.203 kb on - strand at 2177.211 kb on + strand at 2177.211 kb on + strand at 2177.211 kb on + strand at 2177.295 kb on - strand at 2177.321 kb on + strand at 2177.322 kb on - strand at 2177.322 kb on - strand at 2177.337 kb on - strand at 2177.395 kb on + strand, within hokG at 2177.395 kb on + strand, within hokG at 2177.395 kb on + strand, within hokG at 2177.395 kb on + strand, within hokG at 2177.395 kb on + strand, within hokG at 2177.395 kb on + strand, within hokG at 2177.395 kb on + strand, within hokG at 2177.396 kb on - strand, within hokG at 2177.396 kb on - strand, within hokG at 2177.396 kb on - strand, within hokG at 2177.398 kb on + strand, within hokG at 2177.398 kb on + strand, within hokG at 2177.399 kb on - strand, within hokG at 2177.408 kb on + strand, within hokG at 2177.409 kb on - strand, within hokG at 2177.409 kb on - strand, within hokG at 2177.446 kb on + strand, within hokG at 2177.446 kb on + strand, within hokG at 2177.447 kb on - strand, within hokG at 2177.483 kb on + strand, within hokG at 2177.500 kb on - strand, within hokG at 2177.544 kb on - strand at 2177.685 kb on - strand at 2177.713 kb on + strand at 2177.856 kb on - strand at 2177.859 kb on + strand at 2177.859 kb on + strand at 2177.894 kb on + strand at 2177.895 kb on - strand at 2178.003 kb on - strand, within cas3 at 2178.048 kb on - strand, within cas3 at 2178.096 kb on - strand, within cas3
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 2,175,378 + cysI MCAODC_10900 0.47 +0.2 2,175,422 + cysI MCAODC_10900 0.49 -4.2 2,175,423 - cysI MCAODC_10900 0.49 -2.4 2,175,423 - cysI MCAODC_10900 0.49 -3.5 2,175,423 - cysI MCAODC_10900 0.49 -3.3 2,175,504 + cysI MCAODC_10900 0.54 -1.4 2,175,588 + cysI MCAODC_10900 0.59 -3.5 2,175,614 + cysI MCAODC_10900 0.60 -2.1 2,175,614 + cysI MCAODC_10900 0.60 -2.1 2,175,641 + cysI MCAODC_10900 0.62 -0.6 2,175,642 - cysI MCAODC_10900 0.62 -2.8 2,175,756 - cysI MCAODC_10900 0.69 +0.5 2,175,839 - cysI MCAODC_10900 0.73 -2.4 2,175,941 + cysI MCAODC_10900 0.79 -2.5 2,175,961 + cysI MCAODC_10900 0.81 -1.8 2,176,006 + cysI MCAODC_10900 0.83 -2.6 2,176,007 - cysI MCAODC_10900 0.83 -0.9 2,176,081 - cysI MCAODC_10900 0.88 -2.3 2,176,118 + cysI MCAODC_10900 0.90 -2.4 2,176,131 + -3.2 2,176,132 - -0.3 2,176,291 - -1.2 2,176,319 + -1.8 2,176,320 - -2.4 2,176,365 + -2.2 2,176,366 - -4.2 2,176,366 - -2.2 2,176,382 + -3.3 2,176,383 - -1.9 2,176,409 + -3.0 2,176,410 - -3.3 2,176,476 - cysH MCAODC_10905 0.15 -1.7 2,176,507 + cysH MCAODC_10905 0.19 -3.7 2,176,507 + cysH MCAODC_10905 0.19 -3.1 2,176,507 + cysH MCAODC_10905 0.19 -0.8 2,176,507 + cysH MCAODC_10905 0.19 -3.1 2,176,508 - cysH MCAODC_10905 0.19 -2.8 2,176,512 - cysH MCAODC_10905 0.20 -1.6 2,176,578 + cysH MCAODC_10905 0.29 -1.8 2,176,632 + cysH MCAODC_10905 0.36 -2.1 2,176,675 + cysH MCAODC_10905 0.42 -4.1 2,176,756 - cysH MCAODC_10905 0.53 -3.6 2,176,892 + cysH MCAODC_10905 0.72 -3.1 2,176,896 + cysH MCAODC_10905 0.72 -0.9 2,176,897 - cysH MCAODC_10905 0.72 -1.4 2,176,897 - cysH MCAODC_10905 0.72 -2.4 2,176,935 - cysH MCAODC_10905 0.77 -3.8 2,176,942 + cysH MCAODC_10905 0.78 -5.2 2,176,967 - cysH MCAODC_10905 0.82 -2.6 2,177,013 + cysH MCAODC_10905 0.88 -2.0 2,177,117 + +1.7 2,177,203 - +1.2 2,177,211 + -0.7 2,177,211 + -0.6 2,177,211 + +1.3 2,177,295 - +0.1 2,177,321 + +0.2 2,177,322 - +0.3 2,177,322 - +0.2 2,177,337 - +1.2 2,177,395 + hokG MCAODC_10915 0.20 -1.4 2,177,395 + hokG MCAODC_10915 0.20 -0.0 2,177,395 + hokG MCAODC_10915 0.20 -0.7 2,177,395 + hokG MCAODC_10915 0.20 +1.3 2,177,395 + hokG MCAODC_10915 0.20 +0.4 2,177,395 + hokG MCAODC_10915 0.20 -0.1 2,177,395 + hokG MCAODC_10915 0.20 -0.1 2,177,396 - hokG MCAODC_10915 0.21 +1.1 2,177,396 - hokG MCAODC_10915 0.21 -0.1 2,177,396 - hokG MCAODC_10915 0.21 -0.4 2,177,398 + hokG MCAODC_10915 0.22 -0.6 2,177,398 + hokG MCAODC_10915 0.22 +0.1 2,177,399 - hokG MCAODC_10915 0.23 +0.5 2,177,408 + hokG MCAODC_10915 0.29 +0.4 2,177,409 - hokG MCAODC_10915 0.29 -2.5 2,177,409 - hokG MCAODC_10915 0.29 -2.3 2,177,446 + hokG MCAODC_10915 0.54 -0.0 2,177,446 + hokG MCAODC_10915 0.54 +0.3 2,177,447 - hokG MCAODC_10915 0.54 +0.6 2,177,483 + hokG MCAODC_10915 0.78 -0.2 2,177,500 - hokG MCAODC_10915 0.89 +3.8 2,177,544 - +0.0 2,177,685 - +0.5 2,177,713 + -0.7 2,177,856 - -1.0 2,177,859 + +0.4 2,177,859 + +0.5 2,177,894 + -0.0 2,177,895 - -0.2 2,178,003 - cas3 MCAODC_10920 0.11 +1.8 2,178,048 - cas3 MCAODC_10920 0.13 +0.1 2,178,096 - cas3 MCAODC_10920 0.14 +0.2
Or see this region's nucleotide sequence