Strain Fitness in Escherichia coli ECRC101 around MCAODC_10905

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysI and cysH are separated by 73 nucleotidescysH and hokG are separated by 263 nucleotideshokG and cas3 are separated by 191 nucleotides MCAODC_10900: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 2,174,580 to 2,176,292 cysI MCAODC_10905: cysH - phosphoadenosine phosphosulfate reductase, at 2,176,366 to 2,177,100 cysH MCAODC_10915: hokG - Putative protein HokG, at 2,177,364 to 2,177,516 hokG MCAODC_10920: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 2,177,708 to 2,180,407 cas3 Position (kb) 2176 2177 2178Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 2175.378 kb on + strand, within cysIat 2175.422 kb on + strand, within cysIat 2175.423 kb on - strand, within cysIat 2175.423 kb on - strand, within cysIat 2175.423 kb on - strand, within cysIat 2175.504 kb on + strand, within cysIat 2175.588 kb on + strand, within cysIat 2175.614 kb on + strand, within cysIat 2175.614 kb on + strand, within cysIat 2175.641 kb on + strand, within cysIat 2175.642 kb on - strand, within cysIat 2175.756 kb on - strand, within cysIat 2175.839 kb on - strand, within cysIat 2175.941 kb on + strand, within cysIat 2175.961 kb on + strand, within cysIat 2176.006 kb on + strand, within cysIat 2176.007 kb on - strand, within cysIat 2176.081 kb on - strand, within cysIat 2176.118 kb on + strand, within cysIat 2176.131 kb on + strandat 2176.132 kb on - strandat 2176.291 kb on - strandat 2176.319 kb on + strandat 2176.320 kb on - strandat 2176.365 kb on + strandat 2176.366 kb on - strandat 2176.366 kb on - strandat 2176.382 kb on + strandat 2176.383 kb on - strandat 2176.409 kb on + strandat 2176.410 kb on - strandat 2176.476 kb on - strand, within cysHat 2176.507 kb on + strand, within cysHat 2176.507 kb on + strand, within cysHat 2176.507 kb on + strand, within cysHat 2176.507 kb on + strand, within cysHat 2176.508 kb on - strand, within cysHat 2176.512 kb on - strand, within cysHat 2176.578 kb on + strand, within cysHat 2176.632 kb on + strand, within cysHat 2176.675 kb on + strand, within cysHat 2176.756 kb on - strand, within cysHat 2176.892 kb on + strand, within cysHat 2176.896 kb on + strand, within cysHat 2176.897 kb on - strand, within cysHat 2176.897 kb on - strand, within cysHat 2176.935 kb on - strand, within cysHat 2176.942 kb on + strand, within cysHat 2176.967 kb on - strand, within cysHat 2177.013 kb on + strand, within cysHat 2177.117 kb on + strandat 2177.203 kb on - strandat 2177.211 kb on + strandat 2177.211 kb on + strandat 2177.211 kb on + strandat 2177.295 kb on - strandat 2177.321 kb on + strandat 2177.322 kb on - strandat 2177.322 kb on - strandat 2177.337 kb on - strandat 2177.395 kb on + strand, within hokGat 2177.395 kb on + strand, within hokGat 2177.395 kb on + strand, within hokGat 2177.395 kb on + strand, within hokGat 2177.395 kb on + strand, within hokGat 2177.395 kb on + strand, within hokGat 2177.395 kb on + strand, within hokGat 2177.396 kb on - strand, within hokGat 2177.396 kb on - strand, within hokGat 2177.396 kb on - strand, within hokGat 2177.398 kb on + strand, within hokGat 2177.398 kb on + strand, within hokGat 2177.399 kb on - strand, within hokGat 2177.408 kb on + strand, within hokGat 2177.409 kb on - strand, within hokGat 2177.409 kb on - strand, within hokGat 2177.446 kb on + strand, within hokGat 2177.446 kb on + strand, within hokGat 2177.447 kb on - strand, within hokGat 2177.483 kb on + strand, within hokGat 2177.500 kb on - strand, within hokGat 2177.544 kb on - strandat 2177.685 kb on - strandat 2177.713 kb on + strandat 2177.856 kb on - strandat 2177.859 kb on + strandat 2177.859 kb on + strandat 2177.894 kb on + strandat 2177.895 kb on - strandat 2178.003 kb on - strand, within cas3at 2178.048 kb on - strand, within cas3at 2178.096 kb on - strand, within cas3

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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2,175,378 + cysI MCAODC_10900 0.47 +0.2
2,175,422 + cysI MCAODC_10900 0.49 -4.2
2,175,423 - cysI MCAODC_10900 0.49 -2.4
2,175,423 - cysI MCAODC_10900 0.49 -3.5
2,175,423 - cysI MCAODC_10900 0.49 -3.3
2,175,504 + cysI MCAODC_10900 0.54 -1.4
2,175,588 + cysI MCAODC_10900 0.59 -3.5
2,175,614 + cysI MCAODC_10900 0.60 -2.1
2,175,614 + cysI MCAODC_10900 0.60 -2.1
2,175,641 + cysI MCAODC_10900 0.62 -0.6
2,175,642 - cysI MCAODC_10900 0.62 -2.8
2,175,756 - cysI MCAODC_10900 0.69 +0.5
2,175,839 - cysI MCAODC_10900 0.73 -2.4
2,175,941 + cysI MCAODC_10900 0.79 -2.5
2,175,961 + cysI MCAODC_10900 0.81 -1.8
2,176,006 + cysI MCAODC_10900 0.83 -2.6
2,176,007 - cysI MCAODC_10900 0.83 -0.9
2,176,081 - cysI MCAODC_10900 0.88 -2.3
2,176,118 + cysI MCAODC_10900 0.90 -2.4
2,176,131 + -3.2
2,176,132 - -0.3
2,176,291 - -1.2
2,176,319 + -1.8
2,176,320 - -2.4
2,176,365 + -2.2
2,176,366 - -4.2
2,176,366 - -2.2
2,176,382 + -3.3
2,176,383 - -1.9
2,176,409 + -3.0
2,176,410 - -3.3
2,176,476 - cysH MCAODC_10905 0.15 -1.7
2,176,507 + cysH MCAODC_10905 0.19 -3.7
2,176,507 + cysH MCAODC_10905 0.19 -3.1
2,176,507 + cysH MCAODC_10905 0.19 -0.8
2,176,507 + cysH MCAODC_10905 0.19 -3.1
2,176,508 - cysH MCAODC_10905 0.19 -2.8
2,176,512 - cysH MCAODC_10905 0.20 -1.6
2,176,578 + cysH MCAODC_10905 0.29 -1.8
2,176,632 + cysH MCAODC_10905 0.36 -2.1
2,176,675 + cysH MCAODC_10905 0.42 -4.1
2,176,756 - cysH MCAODC_10905 0.53 -3.6
2,176,892 + cysH MCAODC_10905 0.72 -3.1
2,176,896 + cysH MCAODC_10905 0.72 -0.9
2,176,897 - cysH MCAODC_10905 0.72 -1.4
2,176,897 - cysH MCAODC_10905 0.72 -2.4
2,176,935 - cysH MCAODC_10905 0.77 -3.8
2,176,942 + cysH MCAODC_10905 0.78 -5.2
2,176,967 - cysH MCAODC_10905 0.82 -2.6
2,177,013 + cysH MCAODC_10905 0.88 -2.0
2,177,117 + +1.7
2,177,203 - +1.2
2,177,211 + -0.7
2,177,211 + -0.6
2,177,211 + +1.3
2,177,295 - +0.1
2,177,321 + +0.2
2,177,322 - +0.3
2,177,322 - +0.2
2,177,337 - +1.2
2,177,395 + hokG MCAODC_10915 0.20 -1.4
2,177,395 + hokG MCAODC_10915 0.20 -0.0
2,177,395 + hokG MCAODC_10915 0.20 -0.7
2,177,395 + hokG MCAODC_10915 0.20 +1.3
2,177,395 + hokG MCAODC_10915 0.20 +0.4
2,177,395 + hokG MCAODC_10915 0.20 -0.1
2,177,395 + hokG MCAODC_10915 0.20 -0.1
2,177,396 - hokG MCAODC_10915 0.21 +1.1
2,177,396 - hokG MCAODC_10915 0.21 -0.1
2,177,396 - hokG MCAODC_10915 0.21 -0.4
2,177,398 + hokG MCAODC_10915 0.22 -0.6
2,177,398 + hokG MCAODC_10915 0.22 +0.1
2,177,399 - hokG MCAODC_10915 0.23 +0.5
2,177,408 + hokG MCAODC_10915 0.29 +0.4
2,177,409 - hokG MCAODC_10915 0.29 -2.5
2,177,409 - hokG MCAODC_10915 0.29 -2.3
2,177,446 + hokG MCAODC_10915 0.54 -0.0
2,177,446 + hokG MCAODC_10915 0.54 +0.3
2,177,447 - hokG MCAODC_10915 0.54 +0.6
2,177,483 + hokG MCAODC_10915 0.78 -0.2
2,177,500 - hokG MCAODC_10915 0.89 +3.8
2,177,544 - +0.0
2,177,685 - +0.5
2,177,713 + -0.7
2,177,856 - -1.0
2,177,859 + +0.4
2,177,859 + +0.5
2,177,894 + -0.0
2,177,895 - -0.2
2,178,003 - cas3 MCAODC_10920 0.11 +1.8
2,178,048 - cas3 MCAODC_10920 0.13 +0.1
2,178,096 - cas3 MCAODC_10920 0.14 +0.2

Or see this region's nucleotide sequence