Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaJ and rfaB are separated by 8 nucleotides rfaB and rfaL are separated by 35 nucleotides
MCAODC_06185: rfaJ - glucosyltransferase, at 1,238,962 to 1,239,975
rfaJ
MCAODC_06190: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 1,239,984 to 1,241,126
rfaB
MCAODC_06195: rfaL - O-antigen ligase RfaL, at 1,241,162 to 1,242,370
rfaL
Position (kb) 1239
1240
1241
1242 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1238.996 kb on + strand at 1239.045 kb on + strand at 1239.183 kb on - strand, within rfaJ at 1239.338 kb on + strand, within rfaJ at 1239.344 kb on - strand, within rfaJ at 1239.434 kb on + strand, within rfaJ at 1239.435 kb on - strand, within rfaJ at 1239.441 kb on + strand, within rfaJ at 1239.441 kb on + strand, within rfaJ at 1239.493 kb on + strand, within rfaJ at 1239.514 kb on + strand, within rfaJ at 1239.722 kb on - strand, within rfaJ at 1239.835 kb on - strand, within rfaJ at 1239.837 kb on - strand, within rfaJ at 1239.887 kb on - strand at 1240.304 kb on + strand, within rfaB at 1240.364 kb on + strand, within rfaB at 1240.426 kb on + strand, within rfaB at 1240.544 kb on + strand, within rfaB at 1240.683 kb on - strand, within rfaB at 1240.763 kb on + strand, within rfaB at 1240.764 kb on - strand, within rfaB at 1240.764 kb on - strand, within rfaB at 1240.764 kb on - strand, within rfaB at 1240.768 kb on + strand, within rfaB at 1240.800 kb on + strand, within rfaB at 1240.875 kb on - strand, within rfaB at 1240.886 kb on + strand, within rfaB at 1240.886 kb on + strand, within rfaB at 1240.967 kb on + strand, within rfaB at 1241.068 kb on - strand at 1241.111 kb on + strand at 1241.277 kb on + strand at 1241.305 kb on + strand, within rfaL at 1241.359 kb on + strand, within rfaL at 1241.375 kb on + strand, within rfaL at 1241.423 kb on + strand, within rfaL at 1241.483 kb on - strand, within rfaL at 1241.505 kb on + strand, within rfaL at 1241.529 kb on - strand, within rfaL at 1241.579 kb on - strand, within rfaL at 1241.579 kb on - strand, within rfaL at 1241.598 kb on + strand, within rfaL at 1241.599 kb on - strand, within rfaL at 1241.928 kb on + strand, within rfaL at 1242.058 kb on + strand, within rfaL
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 1,238,996 + -4.4 1,239,045 + -3.2 1,239,183 - rfaJ MCAODC_06185 0.22 -4.7 1,239,338 + rfaJ MCAODC_06185 0.37 -2.4 1,239,344 - rfaJ MCAODC_06185 0.38 +0.9 1,239,434 + rfaJ MCAODC_06185 0.47 -3.9 1,239,435 - rfaJ MCAODC_06185 0.47 -1.7 1,239,441 + rfaJ MCAODC_06185 0.47 -1.4 1,239,441 + rfaJ MCAODC_06185 0.47 -3.3 1,239,493 + rfaJ MCAODC_06185 0.52 -4.4 1,239,514 + rfaJ MCAODC_06185 0.54 +0.6 1,239,722 - rfaJ MCAODC_06185 0.75 -3.8 1,239,835 - rfaJ MCAODC_06185 0.86 -2.7 1,239,837 - rfaJ MCAODC_06185 0.86 -4.7 1,239,887 - -4.4 1,240,304 + rfaB MCAODC_06190 0.28 -4.2 1,240,364 + rfaB MCAODC_06190 0.33 -1.8 1,240,426 + rfaB MCAODC_06190 0.39 -0.3 1,240,544 + rfaB MCAODC_06190 0.49 -4.0 1,240,683 - rfaB MCAODC_06190 0.61 -1.1 1,240,763 + rfaB MCAODC_06190 0.68 -3.0 1,240,764 - rfaB MCAODC_06190 0.68 -3.8 1,240,764 - rfaB MCAODC_06190 0.68 -1.7 1,240,764 - rfaB MCAODC_06190 0.68 -2.6 1,240,768 + rfaB MCAODC_06190 0.69 -3.0 1,240,800 + rfaB MCAODC_06190 0.71 -3.4 1,240,875 - rfaB MCAODC_06190 0.78 -3.0 1,240,886 + rfaB MCAODC_06190 0.79 -3.5 1,240,886 + rfaB MCAODC_06190 0.79 -2.6 1,240,967 + rfaB MCAODC_06190 0.86 -3.3 1,241,068 - -1.7 1,241,111 + +0.1 1,241,277 + -0.5 1,241,305 + rfaL MCAODC_06195 0.12 -2.6 1,241,359 + rfaL MCAODC_06195 0.16 -3.6 1,241,375 + rfaL MCAODC_06195 0.18 -3.0 1,241,423 + rfaL MCAODC_06195 0.22 -4.5 1,241,483 - rfaL MCAODC_06195 0.27 -3.7 1,241,505 + rfaL MCAODC_06195 0.28 -0.4 1,241,529 - rfaL MCAODC_06195 0.30 -4.0 1,241,579 - rfaL MCAODC_06195 0.34 -3.0 1,241,579 - rfaL MCAODC_06195 0.34 -1.7 1,241,598 + rfaL MCAODC_06195 0.36 -2.6 1,241,599 - rfaL MCAODC_06195 0.36 -1.5 1,241,928 + rfaL MCAODC_06195 0.63 -3.1 1,242,058 + rfaL MCAODC_06195 0.74 -4.8
Or see this region's nucleotide sequence