Strain Fitness in Escherichia coli ECRC101 around MCAODC_06170

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaG and rfaP overlap by 8 nucleotidesrfaP and waaO are separated by 42 nucleotides MCAODC_06165: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 1,235,240 to 1,236,364 rfaG MCAODC_06170: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 1,236,357 to 1,237,154 rfaP MCAODC_06175: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,237,197 to 1,238,204 waaO Position (kb) 1236 1237 1238Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1235.382 kb on + strand, within rfaGat 1235.416 kb on + strand, within rfaGat 1235.546 kb on + strand, within rfaGat 1235.547 kb on - strand, within rfaGat 1235.610 kb on - strand, within rfaGat 1235.610 kb on - strand, within rfaGat 1235.703 kb on - strand, within rfaGat 1235.728 kb on + strand, within rfaGat 1235.762 kb on + strand, within rfaGat 1235.763 kb on - strand, within rfaGat 1235.931 kb on + strand, within rfaGat 1235.932 kb on - strand, within rfaGat 1235.932 kb on - strand, within rfaGat 1235.932 kb on - strand, within rfaGat 1235.933 kb on + strand, within rfaGat 1235.934 kb on - strand, within rfaGat 1235.951 kb on - strand, within rfaGat 1236.057 kb on + strand, within rfaGat 1236.057 kb on + strand, within rfaGat 1236.132 kb on + strand, within rfaGat 1236.132 kb on + strand, within rfaGat 1236.161 kb on + strand, within rfaGat 1236.161 kb on + strand, within rfaGat 1236.163 kb on + strand, within rfaGat 1236.164 kb on - strand, within rfaGat 1236.231 kb on + strand, within rfaGat 1236.309 kb on + strandat 1236.311 kb on + strandat 1236.312 kb on - strandat 1236.343 kb on - strandat 1236.368 kb on + strandat 1236.495 kb on + strand, within rfaPat 1236.568 kb on + strand, within rfaPat 1236.629 kb on + strand, within rfaPat 1236.629 kb on + strand, within rfaPat 1236.686 kb on + strand, within rfaPat 1236.686 kb on + strand, within rfaPat 1236.687 kb on - strand, within rfaPat 1236.715 kb on + strand, within rfaPat 1236.715 kb on + strand, within rfaPat 1236.782 kb on + strand, within rfaPat 1236.786 kb on - strand, within rfaPat 1236.846 kb on + strand, within rfaPat 1236.847 kb on - strand, within rfaPat 1236.847 kb on - strand, within rfaPat 1236.848 kb on + strand, within rfaPat 1236.849 kb on - strand, within rfaPat 1237.044 kb on + strand, within rfaPat 1237.152 kb on + strandat 1237.282 kb on + strandat 1237.324 kb on + strand, within waaOat 1237.490 kb on - strand, within waaOat 1237.512 kb on + strand, within waaOat 1237.570 kb on + strand, within waaOat 1237.720 kb on + strand, within waaOat 1237.732 kb on - strand, within waaOat 1237.918 kb on - strand, within waaOat 1237.920 kb on - strand, within waaOat 1237.981 kb on + strand, within waaOat 1238.087 kb on - strand, within waaO

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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1,235,382 + rfaG MCAODC_06165 0.13 -0.6
1,235,416 + rfaG MCAODC_06165 0.16 -0.5
1,235,546 + rfaG MCAODC_06165 0.27 -0.6
1,235,547 - rfaG MCAODC_06165 0.27 -0.9
1,235,610 - rfaG MCAODC_06165 0.33 -1.4
1,235,610 - rfaG MCAODC_06165 0.33 -0.1
1,235,703 - rfaG MCAODC_06165 0.41 -3.0
1,235,728 + rfaG MCAODC_06165 0.43 -2.3
1,235,762 + rfaG MCAODC_06165 0.46 -3.2
1,235,763 - rfaG MCAODC_06165 0.46 -2.3
1,235,931 + rfaG MCAODC_06165 0.61 -0.5
1,235,932 - rfaG MCAODC_06165 0.62 +0.6
1,235,932 - rfaG MCAODC_06165 0.62 -0.8
1,235,932 - rfaG MCAODC_06165 0.62 -2.0
1,235,933 + rfaG MCAODC_06165 0.62 -3.1
1,235,934 - rfaG MCAODC_06165 0.62 +0.9
1,235,951 - rfaG MCAODC_06165 0.63 -0.6
1,236,057 + rfaG MCAODC_06165 0.73 +1.4
1,236,057 + rfaG MCAODC_06165 0.73 +0.1
1,236,132 + rfaG MCAODC_06165 0.79 -0.6
1,236,132 + rfaG MCAODC_06165 0.79 -1.8
1,236,161 + rfaG MCAODC_06165 0.82 -1.1
1,236,161 + rfaG MCAODC_06165 0.82 -2.6
1,236,163 + rfaG MCAODC_06165 0.82 -0.8
1,236,164 - rfaG MCAODC_06165 0.82 -0.6
1,236,231 + rfaG MCAODC_06165 0.88 -2.0
1,236,309 + -1.9
1,236,311 + +0.5
1,236,312 - +1.9
1,236,343 - -0.6
1,236,368 + -0.4
1,236,495 + rfaP MCAODC_06170 0.17 +1.5
1,236,568 + rfaP MCAODC_06170 0.26 -0.8
1,236,629 + rfaP MCAODC_06170 0.34 +0.6
1,236,629 + rfaP MCAODC_06170 0.34 -2.2
1,236,686 + rfaP MCAODC_06170 0.41 -2.1
1,236,686 + rfaP MCAODC_06170 0.41 -1.2
1,236,687 - rfaP MCAODC_06170 0.41 -1.1
1,236,715 + rfaP MCAODC_06170 0.45 -0.2
1,236,715 + rfaP MCAODC_06170 0.45 +0.9
1,236,782 + rfaP MCAODC_06170 0.53 -1.4
1,236,786 - rfaP MCAODC_06170 0.54 -0.6
1,236,846 + rfaP MCAODC_06170 0.61 -1.9
1,236,847 - rfaP MCAODC_06170 0.61 -1.1
1,236,847 - rfaP MCAODC_06170 0.61 -1.5
1,236,848 + rfaP MCAODC_06170 0.62 +0.1
1,236,849 - rfaP MCAODC_06170 0.62 -1.0
1,237,044 + rfaP MCAODC_06170 0.86 -0.6
1,237,152 + +0.0
1,237,282 + -3.1
1,237,324 + waaO MCAODC_06175 0.13 -0.4
1,237,490 - waaO MCAODC_06175 0.29 -2.1
1,237,512 + waaO MCAODC_06175 0.31 -3.9
1,237,570 + waaO MCAODC_06175 0.37 -3.8
1,237,720 + waaO MCAODC_06175 0.52 -3.1
1,237,732 - waaO MCAODC_06175 0.53 -2.7
1,237,918 - waaO MCAODC_06175 0.72 -4.6
1,237,920 - waaO MCAODC_06175 0.72 -1.7
1,237,981 + waaO MCAODC_06175 0.78 -3.3
1,238,087 - waaO MCAODC_06175 0.88 -1.1

Or see this region's nucleotide sequence