Strain Fitness in Escherichia coli ECRC101 around MCAODC_05830

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntetgA and grlR are separated by 195 nucleotidesgrlR and grlA are separated by 54 nucleotidesgrlA and cesD are separated by 383 nucleotidescesD and escC are separated by 13 nucleotides MCAODC_05820: etgA - type III secretion system LEE muramidase EtgA, at 1,170,911 to 1,171,369 etgA MCAODC_05825: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 1,171,565 to 1,171,936 grlR MCAODC_05830: grlA - type III secretion system LEE transcriptional regulator GrlA, at 1,171,991 to 1,172,404 grlA MCAODC_05835: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 1,172,788 to 1,173,243 cesD MCAODC_05840: escC - type III secretion system LEE outer membrane ring protein EscC, at 1,173,257 to 1,174,795 escC Position (kb) 1171 1172 1173Strain fitness (log2 ratio) -2 -1 0 1 2at 1171.003 kb on + strand, within etgAat 1171.183 kb on + strand, within etgAat 1171.195 kb on - strand, within etgAat 1171.281 kb on - strand, within etgAat 1171.547 kb on + strandat 1171.570 kb on + strandat 1171.828 kb on - strand, within grlRat 1172.045 kb on + strand, within grlAat 1172.238 kb on - strand, within grlAat 1172.240 kb on - strand, within grlAat 1172.533 kb on + strandat 1172.566 kb on + strandat 1172.579 kb on - strandat 1172.656 kb on + strandat 1172.933 kb on + strand, within cesDat 1172.960 kb on - strand, within cesDat 1172.960 kb on - strand, within cesDat 1173.048 kb on - strand, within cesDat 1173.066 kb on - strand, within cesDat 1173.099 kb on + strand, within cesDat 1173.123 kb on + strand, within cesDat 1173.124 kb on - strand, within cesD

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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1,171,003 + etgA MCAODC_05820 0.20 +0.7
1,171,183 + etgA MCAODC_05820 0.59 -0.1
1,171,195 - etgA MCAODC_05820 0.62 -0.3
1,171,281 - etgA MCAODC_05820 0.81 -0.6
1,171,547 + -1.2
1,171,570 + +0.0
1,171,828 - grlR MCAODC_05825 0.71 -1.4
1,172,045 + grlA MCAODC_05830 0.13 -2.2
1,172,238 - grlA MCAODC_05830 0.60 -0.6
1,172,240 - grlA MCAODC_05830 0.60 -2.7
1,172,533 + +0.3
1,172,566 + -0.4
1,172,579 - +0.4
1,172,656 + -0.9
1,172,933 + cesD MCAODC_05835 0.32 +2.1
1,172,960 - cesD MCAODC_05835 0.38 -0.2
1,172,960 - cesD MCAODC_05835 0.38 -0.3
1,173,048 - cesD MCAODC_05835 0.57 +1.4
1,173,066 - cesD MCAODC_05835 0.61 -0.4
1,173,099 + cesD MCAODC_05835 0.68 -0.0
1,173,123 + cesD MCAODC_05835 0.73 -0.9
1,173,124 - cesD MCAODC_05835 0.74 +0.0

Or see this region's nucleotide sequence