Strain Fitness in Escherichia coli ECRC101 around MCAODC_05825

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescU and etgA overlap by 4 nucleotidesetgA and grlR are separated by 195 nucleotidesgrlR and grlA are separated by 54 nucleotidesgrlA and cesD are separated by 383 nucleotides MCAODC_05815: escU - type III secretion system LEE export apparatus switch protein EscU, at 1,169,877 to 1,170,914 escU MCAODC_05820: etgA - type III secretion system LEE muramidase EtgA, at 1,170,911 to 1,171,369 etgA MCAODC_05825: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 1,171,565 to 1,171,936 grlR MCAODC_05830: grlA - type III secretion system LEE transcriptional regulator GrlA, at 1,171,991 to 1,172,404 grlA MCAODC_05835: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 1,172,788 to 1,173,243 cesD Position (kb) 1171 1172Strain fitness (log2 ratio) -2 -1 0 1 2at 1170.626 kb on + strand, within escUat 1170.630 kb on - strand, within escUat 1170.686 kb on - strand, within escUat 1170.690 kb on + strand, within escUat 1170.692 kb on + strand, within escUat 1170.712 kb on + strand, within escUat 1170.836 kb on + strandat 1170.846 kb on + strandat 1171.003 kb on + strand, within etgAat 1171.183 kb on + strand, within etgAat 1171.195 kb on - strand, within etgAat 1171.281 kb on - strand, within etgAat 1171.547 kb on + strandat 1171.570 kb on + strandat 1171.828 kb on - strand, within grlRat 1172.045 kb on + strand, within grlAat 1172.238 kb on - strand, within grlAat 1172.240 kb on - strand, within grlAat 1172.533 kb on + strandat 1172.566 kb on + strandat 1172.579 kb on - strandat 1172.656 kb on + strandat 1172.933 kb on + strand, within cesD

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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1,170,626 + escU MCAODC_05815 0.72 -2.5
1,170,630 - escU MCAODC_05815 0.73 +0.5
1,170,686 - escU MCAODC_05815 0.78 -0.2
1,170,690 + escU MCAODC_05815 0.78 -0.6
1,170,692 + escU MCAODC_05815 0.79 -1.3
1,170,712 + escU MCAODC_05815 0.80 -1.3
1,170,836 + -0.8
1,170,846 + -0.2
1,171,003 + etgA MCAODC_05820 0.20 +0.7
1,171,183 + etgA MCAODC_05820 0.59 -0.1
1,171,195 - etgA MCAODC_05820 0.62 -0.3
1,171,281 - etgA MCAODC_05820 0.81 -0.6
1,171,547 + -1.2
1,171,570 + +0.0
1,171,828 - grlR MCAODC_05825 0.71 -1.4
1,172,045 + grlA MCAODC_05830 0.13 -2.2
1,172,238 - grlA MCAODC_05830 0.60 -0.6
1,172,240 - grlA MCAODC_05830 0.60 -2.7
1,172,533 + +0.3
1,172,566 + -0.4
1,172,579 - +0.4
1,172,656 + -0.9
1,172,933 + cesD MCAODC_05835 0.32 +2.1

Or see this region's nucleotide sequence