Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceB and metA are separated by 268 nucleotides metA and yjaB are separated by 156 nucleotides yjaB and 5S_rrna are separated by 116 nucleotides 5S_rrna and 23S_rrna are separated by 94 nucleotides
MCAODC_03770: aceB - malate synthase A, at 760,673 to 762,274
aceB
MCAODC_03775: metA - homoserine O-succinyltransferase, at 762,543 to 763,472
metA
MCAODC_03780: yjaB - acetyltransferase, at 763,629 to 764,072
yjaB
MCAODC_03785: 5S_rrna - 5S ribosomal RNA, at 764,189 to 764,304
_rrna
MCAODC_03790: 23S_rrna - 23S ribosomal RNA, at 764,399 to 767,303
_rrna
Position (kb) 762
763
764 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 761.581 kb on + strand, within aceB at 761.583 kb on + strand, within aceB at 761.584 kb on - strand, within aceB at 761.640 kb on + strand, within aceB at 761.646 kb on + strand, within aceB at 761.646 kb on + strand, within aceB at 761.647 kb on - strand, within aceB at 761.679 kb on + strand, within aceB at 761.680 kb on - strand, within aceB at 761.680 kb on - strand, within aceB at 761.695 kb on - strand, within aceB at 761.695 kb on - strand, within aceB at 761.838 kb on + strand, within aceB at 761.838 kb on + strand, within aceB at 761.839 kb on - strand, within aceB at 761.981 kb on + strand, within aceB at 762.015 kb on - strand, within aceB at 762.015 kb on - strand, within aceB at 762.139 kb on + strand at 762.199 kb on + strand at 762.226 kb on - strand at 762.234 kb on - strand at 762.355 kb on - strand at 762.503 kb on + strand at 762.531 kb on + strand at 762.593 kb on - strand at 762.598 kb on + strand at 762.598 kb on + strand at 762.599 kb on - strand at 762.601 kb on + strand at 762.602 kb on - strand at 762.685 kb on + strand, within metA at 762.686 kb on - strand, within metA at 762.686 kb on - strand, within metA at 762.689 kb on + strand, within metA at 762.689 kb on + strand, within metA at 762.689 kb on + strand, within metA at 762.690 kb on - strand, within metA at 762.690 kb on - strand, within metA at 762.690 kb on - strand, within metA at 762.691 kb on + strand, within metA at 762.691 kb on + strand, within metA at 762.691 kb on + strand, within metA at 762.691 kb on + strand, within metA at 762.692 kb on - strand, within metA at 762.692 kb on - strand, within metA at 762.696 kb on + strand, within metA at 762.760 kb on + strand, within metA at 762.795 kb on - strand, within metA at 762.795 kb on - strand, within metA at 762.814 kb on + strand, within metA at 762.814 kb on + strand, within metA at 762.814 kb on + strand, within metA at 762.814 kb on + strand, within metA at 762.814 kb on + strand, within metA at 762.815 kb on - strand, within metA at 762.815 kb on - strand, within metA at 762.815 kb on - strand, within metA at 762.815 kb on - strand, within metA at 762.859 kb on + strand, within metA at 762.860 kb on - strand, within metA at 762.970 kb on + strand, within metA at 762.971 kb on - strand, within metA at 763.004 kb on + strand, within metA at 763.004 kb on + strand, within metA at 763.128 kb on - strand, within metA at 763.159 kb on - strand, within metA at 763.207 kb on + strand, within metA at 763.364 kb on + strand, within metA at 763.365 kb on - strand, within metA at 763.365 kb on - strand, within metA at 763.365 kb on - strand, within metA at 763.365 kb on - strand, within metA at 763.365 kb on - strand, within metA at 763.365 kb on - strand, within metA at 763.494 kb on - strand at 763.494 kb on - strand at 763.598 kb on + strand at 763.598 kb on + strand at 763.599 kb on - strand at 763.605 kb on + strand at 763.605 kb on + strand at 763.606 kb on - strand at 763.637 kb on + strand at 763.640 kb on + strand at 763.824 kb on - strand, within yjaB at 763.831 kb on + strand, within yjaB at 763.832 kb on - strand, within yjaB at 763.832 kb on - strand, within yjaB at 763.844 kb on + strand, within yjaB at 763.844 kb on + strand, within yjaB at 763.844 kb on + strand, within yjaB at 763.844 kb on + strand, within yjaB at 763.859 kb on + strand, within yjaB at 763.977 kb on + strand, within yjaB at 763.978 kb on - strand, within yjaB at 763.994 kb on + strand, within yjaB at 764.059 kb on - strand at 764.059 kb on - strand at 764.071 kb on - strand at 764.095 kb on + strand at 764.096 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 761,581 + aceB MCAODC_03770 0.57 +0.2 761,583 + aceB MCAODC_03770 0.57 -0.7 761,584 - aceB MCAODC_03770 0.57 -0.8 761,640 + aceB MCAODC_03770 0.60 +0.2 761,646 + aceB MCAODC_03770 0.61 -0.2 761,646 + aceB MCAODC_03770 0.61 +0.7 761,647 - aceB MCAODC_03770 0.61 -1.6 761,679 + aceB MCAODC_03770 0.63 +0.3 761,680 - aceB MCAODC_03770 0.63 -0.3 761,680 - aceB MCAODC_03770 0.63 +0.5 761,695 - aceB MCAODC_03770 0.64 -0.9 761,695 - aceB MCAODC_03770 0.64 -1.2 761,838 + aceB MCAODC_03770 0.73 -1.8 761,838 + aceB MCAODC_03770 0.73 +1.1 761,839 - aceB MCAODC_03770 0.73 +2.3 761,981 + aceB MCAODC_03770 0.82 -0.1 762,015 - aceB MCAODC_03770 0.84 +0.3 762,015 - aceB MCAODC_03770 0.84 -0.9 762,139 + -2.5 762,199 + +0.4 762,226 - -0.0 762,234 - +0.4 762,355 - +1.0 762,503 + -0.7 762,531 + +1.0 762,593 - +1.4 762,598 + -2.1 762,598 + -2.2 762,599 - -1.1 762,601 + -3.6 762,602 - -1.0 762,685 + metA MCAODC_03775 0.15 +1.4 762,686 - metA MCAODC_03775 0.15 +0.3 762,686 - metA MCAODC_03775 0.15 +1.0 762,689 + metA MCAODC_03775 0.16 +0.4 762,689 + metA MCAODC_03775 0.16 -4.5 762,689 + metA MCAODC_03775 0.16 -1.1 762,690 - metA MCAODC_03775 0.16 -2.5 762,690 - metA MCAODC_03775 0.16 -0.0 762,690 - metA MCAODC_03775 0.16 -2.1 762,691 + metA MCAODC_03775 0.16 -3.9 762,691 + metA MCAODC_03775 0.16 -2.4 762,691 + metA MCAODC_03775 0.16 -3.2 762,691 + metA MCAODC_03775 0.16 -3.7 762,692 - metA MCAODC_03775 0.16 -0.5 762,692 - metA MCAODC_03775 0.16 -0.9 762,696 + metA MCAODC_03775 0.16 -2.3 762,760 + metA MCAODC_03775 0.23 -2.0 762,795 - metA MCAODC_03775 0.27 -2.1 762,795 - metA MCAODC_03775 0.27 -1.2 762,814 + metA MCAODC_03775 0.29 -1.9 762,814 + metA MCAODC_03775 0.29 -3.0 762,814 + metA MCAODC_03775 0.29 -1.4 762,814 + metA MCAODC_03775 0.29 -1.9 762,814 + metA MCAODC_03775 0.29 -2.4 762,815 - metA MCAODC_03775 0.29 -2.9 762,815 - metA MCAODC_03775 0.29 -2.6 762,815 - metA MCAODC_03775 0.29 -4.2 762,815 - metA MCAODC_03775 0.29 -3.0 762,859 + metA MCAODC_03775 0.34 -1.9 762,860 - metA MCAODC_03775 0.34 -0.7 762,970 + metA MCAODC_03775 0.46 -4.5 762,971 - metA MCAODC_03775 0.46 -2.1 763,004 + metA MCAODC_03775 0.50 -1.7 763,004 + metA MCAODC_03775 0.50 -2.7 763,128 - metA MCAODC_03775 0.63 -1.8 763,159 - metA MCAODC_03775 0.66 -2.5 763,207 + metA MCAODC_03775 0.71 -3.6 763,364 + metA MCAODC_03775 0.88 +2.0 763,365 - metA MCAODC_03775 0.88 -2.3 763,365 - metA MCAODC_03775 0.88 -1.9 763,365 - metA MCAODC_03775 0.88 -0.0 763,365 - metA MCAODC_03775 0.88 -2.0 763,365 - metA MCAODC_03775 0.88 -3.1 763,365 - metA MCAODC_03775 0.88 -2.6 763,494 - -0.2 763,494 - -0.6 763,598 + -0.8 763,598 + +0.4 763,599 - -0.7 763,605 + -0.4 763,605 + +0.1 763,606 - -0.3 763,637 + -0.5 763,640 + +0.3 763,824 - yjaB MCAODC_03780 0.44 -0.5 763,831 + yjaB MCAODC_03780 0.45 -0.1 763,832 - yjaB MCAODC_03780 0.46 -0.6 763,832 - yjaB MCAODC_03780 0.46 -0.5 763,844 + yjaB MCAODC_03780 0.48 +1.8 763,844 + yjaB MCAODC_03780 0.48 +0.2 763,844 + yjaB MCAODC_03780 0.48 +0.1 763,844 + yjaB MCAODC_03780 0.48 -0.0 763,859 + yjaB MCAODC_03780 0.52 +0.0 763,977 + yjaB MCAODC_03780 0.78 +0.9 763,978 - yjaB MCAODC_03780 0.79 -0.8 763,994 + yjaB MCAODC_03780 0.82 -0.8 764,059 - -0.2 764,059 - +1.2 764,071 - -0.1 764,095 + -2.5 764,096 - -0.2
Or see this region's nucleotide sequence