Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsmA and apaG are separated by 2 nucleotides apaG and apaH are separated by 6 nucleotides apaH and MCAODC_01365 overlap by 4 nucleotides MCAODC_01365 and MCAODC_01370 are separated by 2 nucleotides MCAODC_01370 and MCAODC_01375 are separated by 85 nucleotides
MCAODC_01350: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 259,790 to 260,611
rsmA
MCAODC_01355: apaG - Co2+/Mg2+ efflux protein ApaG, at 260,614 to 260,991
apaG
MCAODC_01360: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 260,998 to 261,846
apaH
MCAODC_01365: MCAODC_01365 - plasmid maintenance protein CcdB, at 261,843 to 262,157
_01365
MCAODC_01370: MCAODC_01370 - antitoxin, at 262,160 to 262,393
_01370
MCAODC_01375: MCAODC_01375 - Dihydrofolate reductase, at 262,479 to 263,069
_01375
Position (kb) 260
261
262 Strain fitness (log2 ratio) -1
0
1
2
3 at 260.135 kb on + strand, within rsmA at 260.137 kb on + strand, within rsmA at 260.137 kb on + strand, within rsmA at 260.138 kb on - strand, within rsmA at 260.170 kb on + strand, within rsmA at 260.174 kb on + strand, within rsmA at 260.288 kb on + strand, within rsmA at 260.467 kb on + strand, within rsmA at 260.576 kb on + strand at 260.637 kb on + strand at 260.657 kb on - strand, within apaG at 260.692 kb on + strand, within apaG at 260.704 kb on + strand, within apaG at 260.705 kb on - strand, within apaG at 260.705 kb on - strand, within apaG at 260.706 kb on + strand, within apaG at 260.706 kb on + strand, within apaG at 260.717 kb on + strand, within apaG at 260.857 kb on + strand, within apaG at 260.858 kb on - strand, within apaG at 261.007 kb on + strand at 261.007 kb on + strand at 261.007 kb on + strand at 261.007 kb on + strand at 261.008 kb on - strand at 261.008 kb on - strand at 261.173 kb on + strand, within apaH at 261.173 kb on + strand, within apaH at 261.174 kb on - strand, within apaH at 261.294 kb on + strand, within apaH at 261.580 kb on + strand, within apaH at 261.868 kb on - strand at 261.868 kb on - strand at 261.868 kb on - strand at 261.878 kb on + strand, within MCAODC_01365 at 261.879 kb on - strand, within MCAODC_01365 at 261.967 kb on + strand, within MCAODC_01365 at 261.967 kb on + strand, within MCAODC_01365 at 261.967 kb on + strand, within MCAODC_01365 at 261.968 kb on - strand, within MCAODC_01365 at 261.968 kb on - strand, within MCAODC_01365 at 261.988 kb on - strand, within MCAODC_01365 at 262.040 kb on + strand, within MCAODC_01365 at 262.041 kb on - strand, within MCAODC_01365 at 262.041 kb on - strand, within MCAODC_01365 at 262.041 kb on - strand, within MCAODC_01365 at 262.082 kb on + strand, within MCAODC_01365 at 262.082 kb on + strand, within MCAODC_01365 at 262.142 kb on - strand at 262.142 kb on - strand at 262.148 kb on + strand at 262.149 kb on - strand at 262.161 kb on + strand at 262.225 kb on + strand, within MCAODC_01370 at 262.310 kb on - strand, within MCAODC_01370 at 262.313 kb on + strand, within MCAODC_01370 at 262.314 kb on - strand, within MCAODC_01370 at 262.314 kb on - strand, within MCAODC_01370 at 262.341 kb on - strand, within MCAODC_01370 at 262.419 kb on - strand at 262.424 kb on + strand at 262.427 kb on + strand at 262.428 kb on - strand at 262.448 kb on - strand at 262.448 kb on - strand at 262.448 kb on - strand at 262.467 kb on + strand at 262.467 kb on + strand at 262.468 kb on - strand at 262.468 kb on - strand at 262.470 kb on + strand at 262.472 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 260,135 + rsmA MCAODC_01350 0.42 +0.1 260,137 + rsmA MCAODC_01350 0.42 +0.5 260,137 + rsmA MCAODC_01350 0.42 +0.5 260,138 - rsmA MCAODC_01350 0.42 +1.9 260,170 + rsmA MCAODC_01350 0.46 -0.5 260,174 + rsmA MCAODC_01350 0.47 +0.1 260,288 + rsmA MCAODC_01350 0.61 +1.7 260,467 + rsmA MCAODC_01350 0.82 -0.3 260,576 + +0.8 260,637 + -1.4 260,657 - apaG MCAODC_01355 0.11 -0.5 260,692 + apaG MCAODC_01355 0.21 +0.7 260,704 + apaG MCAODC_01355 0.24 +1.2 260,705 - apaG MCAODC_01355 0.24 -0.5 260,705 - apaG MCAODC_01355 0.24 +0.5 260,706 + apaG MCAODC_01355 0.24 +1.6 260,706 + apaG MCAODC_01355 0.24 -0.0 260,717 + apaG MCAODC_01355 0.27 +1.0 260,857 + apaG MCAODC_01355 0.64 +0.2 260,858 - apaG MCAODC_01355 0.65 -0.8 261,007 + +0.5 261,007 + -0.8 261,007 + -0.8 261,007 + +0.5 261,008 - -0.5 261,008 - +0.5 261,173 + apaH MCAODC_01360 0.21 +2.9 261,173 + apaH MCAODC_01360 0.21 -0.9 261,174 - apaH MCAODC_01360 0.21 -0.9 261,294 + apaH MCAODC_01360 0.35 -0.5 261,580 + apaH MCAODC_01360 0.69 +0.9 261,868 - -0.2 261,868 - -0.4 261,868 - +1.4 261,878 + MCAODC_01365 0.11 +0.9 261,879 - MCAODC_01365 0.11 -0.3 261,967 + MCAODC_01365 0.39 -0.3 261,967 + MCAODC_01365 0.39 -0.9 261,967 + MCAODC_01365 0.39 +1.9 261,968 - MCAODC_01365 0.40 +0.2 261,968 - MCAODC_01365 0.40 -0.3 261,988 - MCAODC_01365 0.46 +0.5 262,040 + MCAODC_01365 0.63 -0.5 262,041 - MCAODC_01365 0.63 -0.3 262,041 - MCAODC_01365 0.63 -0.8 262,041 - MCAODC_01365 0.63 -0.7 262,082 + MCAODC_01365 0.76 -0.4 262,082 + MCAODC_01365 0.76 +0.7 262,142 - +0.2 262,142 - -1.3 262,148 + +0.8 262,149 - +1.1 262,161 + +0.5 262,225 + MCAODC_01370 0.28 -0.0 262,310 - MCAODC_01370 0.64 -0.1 262,313 + MCAODC_01370 0.65 +0.5 262,314 - MCAODC_01370 0.66 -1.2 262,314 - MCAODC_01370 0.66 -1.2 262,341 - MCAODC_01370 0.77 -0.3 262,419 - +0.1 262,424 + -0.3 262,427 + +0.2 262,428 - +0.1 262,448 - +1.2 262,448 - -1.6 262,448 - -0.4 262,467 + +0.3 262,467 + +0.3 262,468 - -1.1 262,468 - -0.1 262,470 + +0.4 262,472 + +0.6
Or see this region's nucleotide sequence