Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuO and leuL are separated by 657 nucleotides leuL and MCAODC_01230 are separated by 92 nucleotides MCAODC_01230 and leuB overlap by 1 nucleotides
MCAODC_01220: leuO - transcriptional regulator LeuO, at 227,293 to 228,237
leuO
MCAODC_01225: leuL - leu operon leader peptide, at 228,895 to 228,981
leuL
MCAODC_01230: MCAODC_01230 - 2-isopropylmalate synthase, at 229,074 to 230,645
_01230
MCAODC_01235: leuB - 3-isopropylmalate dehydrogenase, at 230,645 to 231,736
leuB
Position (kb) 229
230
231 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 228.076 kb on - strand, within leuO at 228.160 kb on + strand at 228.160 kb on + strand at 228.161 kb on - strand at 228.161 kb on - strand at 228.182 kb on - strand at 228.182 kb on - strand at 228.241 kb on + strand at 228.242 kb on - strand at 228.305 kb on + strand at 228.426 kb on + strand at 228.461 kb on + strand at 228.505 kb on - strand at 228.537 kb on + strand at 228.679 kb on - strand at 228.743 kb on - strand at 228.757 kb on + strand at 228.775 kb on + strand at 228.786 kb on - strand at 228.862 kb on - strand at 228.894 kb on - strand at 228.894 kb on - strand at 228.923 kb on + strand, within leuL at 228.923 kb on + strand, within leuL at 228.924 kb on - strand, within leuL at 228.924 kb on - strand, within leuL at 228.926 kb on + strand, within leuL at 228.930 kb on - strand, within leuL at 228.954 kb on + strand, within leuL at 229.091 kb on + strand at 229.283 kb on - strand, within MCAODC_01230 at 229.283 kb on - strand, within MCAODC_01230 at 229.283 kb on - strand, within MCAODC_01230 at 229.283 kb on - strand, within MCAODC_01230 at 229.283 kb on - strand, within MCAODC_01230 at 229.283 kb on - strand, within MCAODC_01230 at 229.283 kb on - strand, within MCAODC_01230 at 229.364 kb on + strand, within MCAODC_01230 at 229.370 kb on + strand, within MCAODC_01230 at 229.370 kb on + strand, within MCAODC_01230 at 229.440 kb on - strand, within MCAODC_01230 at 229.443 kb on + strand, within MCAODC_01230 at 229.443 kb on + strand, within MCAODC_01230 at 229.443 kb on + strand, within MCAODC_01230 at 229.443 kb on + strand, within MCAODC_01230 at 229.443 kb on + strand, within MCAODC_01230 at 229.443 kb on + strand, within MCAODC_01230 at 229.444 kb on - strand, within MCAODC_01230 at 229.444 kb on - strand, within MCAODC_01230 at 229.444 kb on - strand, within MCAODC_01230 at 229.444 kb on - strand, within MCAODC_01230 at 229.444 kb on - strand, within MCAODC_01230 at 229.444 kb on - strand, within MCAODC_01230 at 229.444 kb on - strand, within MCAODC_01230 at 229.445 kb on + strand, within MCAODC_01230 at 229.445 kb on + strand, within MCAODC_01230 at 229.446 kb on - strand, within MCAODC_01230 at 229.446 kb on - strand, within MCAODC_01230 at 229.463 kb on + strand, within MCAODC_01230 at 229.467 kb on + strand, within MCAODC_01230 at 229.593 kb on + strand, within MCAODC_01230 at 229.593 kb on + strand, within MCAODC_01230 at 229.636 kb on - strand, within MCAODC_01230 at 229.649 kb on + strand, within MCAODC_01230 at 229.667 kb on + strand, within MCAODC_01230 at 229.676 kb on - strand, within MCAODC_01230 at 229.680 kb on - strand, within MCAODC_01230 at 229.680 kb on - strand, within MCAODC_01230 at 229.680 kb on - strand, within MCAODC_01230 at 229.680 kb on - strand, within MCAODC_01230 at 229.726 kb on + strand, within MCAODC_01230 at 229.727 kb on - strand, within MCAODC_01230 at 229.818 kb on - strand, within MCAODC_01230 at 229.833 kb on - strand, within MCAODC_01230 at 229.857 kb on - strand, within MCAODC_01230 at 229.888 kb on + strand, within MCAODC_01230 at 229.888 kb on + strand, within MCAODC_01230 at 229.889 kb on - strand, within MCAODC_01230 at 229.889 kb on - strand, within MCAODC_01230 at 229.889 kb on - strand, within MCAODC_01230 at 229.893 kb on - strand, within MCAODC_01230 at 229.904 kb on + strand, within MCAODC_01230 at 229.904 kb on + strand, within MCAODC_01230 at 229.904 kb on + strand, within MCAODC_01230 at 229.904 kb on + strand, within MCAODC_01230 at 229.907 kb on + strand, within MCAODC_01230 at 230.005 kb on - strand, within MCAODC_01230 at 230.027 kb on + strand, within MCAODC_01230 at 230.027 kb on + strand, within MCAODC_01230 at 230.028 kb on - strand, within MCAODC_01230 at 230.110 kb on - strand, within MCAODC_01230 at 230.112 kb on - strand, within MCAODC_01230 at 230.126 kb on + strand, within MCAODC_01230 at 230.279 kb on + strand, within MCAODC_01230 at 230.370 kb on + strand, within MCAODC_01230 at 230.399 kb on + strand, within MCAODC_01230 at 230.399 kb on + strand, within MCAODC_01230 at 230.400 kb on - strand, within MCAODC_01230 at 230.400 kb on - strand, within MCAODC_01230 at 230.468 kb on + strand, within MCAODC_01230 at 230.468 kb on + strand, within MCAODC_01230 at 230.481 kb on + strand, within MCAODC_01230 at 230.482 kb on - strand, within MCAODC_01230 at 230.482 kb on - strand, within MCAODC_01230 at 230.482 kb on - strand, within MCAODC_01230 at 230.482 kb on - strand, within MCAODC_01230 at 230.522 kb on + strand at 230.557 kb on + strand at 230.557 kb on + strand at 230.557 kb on + strand at 230.558 kb on - strand at 230.558 kb on - strand at 230.558 kb on - strand at 230.558 kb on - strand at 230.561 kb on + strand at 230.561 kb on + strand at 230.561 kb on + strand at 230.561 kb on + strand at 230.561 kb on + strand at 230.561 kb on + strand at 230.562 kb on - strand at 230.562 kb on - strand at 230.562 kb on - strand at 230.568 kb on - strand at 230.657 kb on + strand at 230.657 kb on + strand at 230.663 kb on - strand at 230.771 kb on + strand, within leuB at 230.779 kb on - strand, within leuB at 230.779 kb on - strand, within leuB at 230.779 kb on - strand, within leuB at 230.779 kb on - strand, within leuB at 230.779 kb on - strand, within leuB at 230.779 kb on - strand, within leuB at 230.895 kb on + strand, within leuB at 230.896 kb on - strand, within leuB at 230.955 kb on + strand, within leuB at 231.077 kb on + strand, within leuB at 231.078 kb on - strand, within leuB at 231.078 kb on - strand, within leuB at 231.116 kb on + strand, within leuB at 231.117 kb on - strand, within leuB at 231.117 kb on - strand, within leuB at 231.144 kb on - strand, within leuB at 231.144 kb on - strand, within leuB at 231.144 kb on - strand, within leuB at 231.166 kb on + strand, within leuB at 231.166 kb on + strand, within leuB at 231.166 kb on + strand, within leuB at 231.167 kb on - strand, within leuB at 231.167 kb on - strand, within leuB at 231.196 kb on + strand, within leuB at 231.209 kb on - strand, within leuB at 231.256 kb on - strand, within leuB at 231.313 kb on + strand, within leuB at 231.313 kb on + strand, within leuB at 231.314 kb on - strand, within leuB at 231.314 kb on - strand, within leuB at 231.317 kb on + strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.318 kb on - strand, within leuB at 231.346 kb on + strand, within leuB at 231.482 kb on + strand, within leuB at 231.482 kb on + strand, within leuB at 231.482 kb on + strand, within leuB at 231.482 kb on + strand, within leuB at 231.482 kb on + strand, within leuB at 231.482 kb on + strand, within leuB at 231.483 kb on - strand, within leuB at 231.483 kb on - strand, within leuB at 231.521 kb on - strand, within leuB at 231.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 228,076 - leuO MCAODC_01220 0.83 +0.2 228,160 + -0.1 228,160 + -0.4 228,161 - -1.4 228,161 - +0.7 228,182 - -1.6 228,182 - -0.3 228,241 + -0.2 228,242 - -0.8 228,305 + +0.1 228,426 + +1.5 228,461 + -0.4 228,505 - +1.9 228,537 + +1.3 228,679 - -0.0 228,743 - +1.1 228,757 + -0.4 228,775 + +0.2 228,786 - +0.3 228,862 - -2.3 228,894 - -3.5 228,894 - -4.0 228,923 + leuL MCAODC_01225 0.32 -1.3 228,923 + leuL MCAODC_01225 0.32 -2.5 228,924 - leuL MCAODC_01225 0.33 -2.0 228,924 - leuL MCAODC_01225 0.33 +1.3 228,926 + leuL MCAODC_01225 0.36 -0.4 228,930 - leuL MCAODC_01225 0.40 -3.2 228,954 + leuL MCAODC_01225 0.68 -3.0 229,091 + -2.9 229,283 - MCAODC_01230 0.13 -3.6 229,283 - MCAODC_01230 0.13 -3.6 229,283 - MCAODC_01230 0.13 -2.0 229,283 - MCAODC_01230 0.13 -2.8 229,283 - MCAODC_01230 0.13 -2.2 229,283 - MCAODC_01230 0.13 -1.4 229,283 - MCAODC_01230 0.13 -3.0 229,364 + MCAODC_01230 0.18 -1.2 229,370 + MCAODC_01230 0.19 -2.1 229,370 + MCAODC_01230 0.19 -2.6 229,440 - MCAODC_01230 0.23 -3.2 229,443 + MCAODC_01230 0.23 -2.5 229,443 + MCAODC_01230 0.23 -4.0 229,443 + MCAODC_01230 0.23 -1.8 229,443 + MCAODC_01230 0.23 -3.5 229,443 + MCAODC_01230 0.23 -2.6 229,443 + MCAODC_01230 0.23 -3.5 229,444 - MCAODC_01230 0.24 -3.3 229,444 - MCAODC_01230 0.24 -1.9 229,444 - MCAODC_01230 0.24 -2.1 229,444 - MCAODC_01230 0.24 -4.9 229,444 - MCAODC_01230 0.24 -2.0 229,444 - MCAODC_01230 0.24 -1.7 229,444 - MCAODC_01230 0.24 -2.3 229,445 + MCAODC_01230 0.24 -4.1 229,445 + MCAODC_01230 0.24 +0.5 229,446 - MCAODC_01230 0.24 -1.8 229,446 - MCAODC_01230 0.24 -1.3 229,463 + MCAODC_01230 0.25 -1.3 229,467 + MCAODC_01230 0.25 -3.4 229,593 + MCAODC_01230 0.33 -3.9 229,593 + MCAODC_01230 0.33 -2.6 229,636 - MCAODC_01230 0.36 +0.3 229,649 + MCAODC_01230 0.37 -1.3 229,667 + MCAODC_01230 0.38 -3.2 229,676 - MCAODC_01230 0.38 -1.8 229,680 - MCAODC_01230 0.39 -1.5 229,680 - MCAODC_01230 0.39 -3.0 229,680 - MCAODC_01230 0.39 -4.1 229,680 - MCAODC_01230 0.39 -3.7 229,726 + MCAODC_01230 0.41 -0.4 229,727 - MCAODC_01230 0.42 -3.8 229,818 - MCAODC_01230 0.47 -3.7 229,833 - MCAODC_01230 0.48 -2.9 229,857 - MCAODC_01230 0.50 -3.4 229,888 + MCAODC_01230 0.52 -3.4 229,888 + MCAODC_01230 0.52 -4.3 229,889 - MCAODC_01230 0.52 -3.3 229,889 - MCAODC_01230 0.52 -3.1 229,889 - MCAODC_01230 0.52 -3.4 229,893 - MCAODC_01230 0.52 -1.4 229,904 + MCAODC_01230 0.53 -2.7 229,904 + MCAODC_01230 0.53 -2.0 229,904 + MCAODC_01230 0.53 -1.8 229,904 + MCAODC_01230 0.53 -4.2 229,907 + MCAODC_01230 0.53 +1.9 230,005 - MCAODC_01230 0.59 -2.2 230,027 + MCAODC_01230 0.61 -4.4 230,027 + MCAODC_01230 0.61 -3.5 230,028 - MCAODC_01230 0.61 -1.8 230,110 - MCAODC_01230 0.66 -2.8 230,112 - MCAODC_01230 0.66 -3.9 230,126 + MCAODC_01230 0.67 -2.3 230,279 + MCAODC_01230 0.77 +0.5 230,370 + MCAODC_01230 0.82 +0.9 230,399 + MCAODC_01230 0.84 -0.7 230,399 + MCAODC_01230 0.84 -0.6 230,400 - MCAODC_01230 0.84 +1.2 230,400 - MCAODC_01230 0.84 -2.4 230,468 + MCAODC_01230 0.89 -0.7 230,468 + MCAODC_01230 0.89 -0.1 230,481 + MCAODC_01230 0.90 -0.9 230,482 - MCAODC_01230 0.90 -5.0 230,482 - MCAODC_01230 0.90 -3.0 230,482 - MCAODC_01230 0.90 -1.1 230,482 - MCAODC_01230 0.90 -1.2 230,522 + -0.4 230,557 + -1.3 230,557 + -1.7 230,557 + -0.1 230,558 - -0.5 230,558 - -0.3 230,558 - -2.7 230,558 - -2.5 230,561 + -0.0 230,561 + +0.5 230,561 + -1.0 230,561 + -1.5 230,561 + +0.3 230,561 + +2.0 230,562 - -2.3 230,562 - -2.8 230,562 - -1.4 230,568 - -3.6 230,657 + -2.8 230,657 + -3.3 230,663 - -4.4 230,771 + leuB MCAODC_01235 0.12 -2.0 230,779 - leuB MCAODC_01235 0.12 -3.0 230,779 - leuB MCAODC_01235 0.12 -1.7 230,779 - leuB MCAODC_01235 0.12 -0.9 230,779 - leuB MCAODC_01235 0.12 -3.3 230,779 - leuB MCAODC_01235 0.12 -2.8 230,779 - leuB MCAODC_01235 0.12 -1.8 230,895 + leuB MCAODC_01235 0.23 -3.5 230,896 - leuB MCAODC_01235 0.23 -0.5 230,955 + leuB MCAODC_01235 0.28 -1.3 231,077 + leuB MCAODC_01235 0.40 -3.2 231,078 - leuB MCAODC_01235 0.40 -3.4 231,078 - leuB MCAODC_01235 0.40 -3.8 231,116 + leuB MCAODC_01235 0.43 -2.0 231,117 - leuB MCAODC_01235 0.43 -3.0 231,117 - leuB MCAODC_01235 0.43 -4.0 231,144 - leuB MCAODC_01235 0.46 -4.2 231,144 - leuB MCAODC_01235 0.46 -3.8 231,144 - leuB MCAODC_01235 0.46 -3.2 231,166 + leuB MCAODC_01235 0.48 -1.7 231,166 + leuB MCAODC_01235 0.48 -2.5 231,166 + leuB MCAODC_01235 0.48 -2.3 231,167 - leuB MCAODC_01235 0.48 -2.9 231,167 - leuB MCAODC_01235 0.48 -4.0 231,196 + leuB MCAODC_01235 0.50 -1.5 231,209 - leuB MCAODC_01235 0.52 -2.0 231,256 - leuB MCAODC_01235 0.56 -1.4 231,313 + leuB MCAODC_01235 0.61 -3.3 231,313 + leuB MCAODC_01235 0.61 +0.2 231,314 - leuB MCAODC_01235 0.61 -0.8 231,314 - leuB MCAODC_01235 0.61 -2.6 231,317 + leuB MCAODC_01235 0.62 -2.4 231,318 - leuB MCAODC_01235 0.62 -1.7 231,318 - leuB MCAODC_01235 0.62 -2.8 231,318 - leuB MCAODC_01235 0.62 -2.7 231,318 - leuB MCAODC_01235 0.62 -2.1 231,318 - leuB MCAODC_01235 0.62 -2.4 231,318 - leuB MCAODC_01235 0.62 -2.3 231,318 - leuB MCAODC_01235 0.62 -1.7 231,318 - leuB MCAODC_01235 0.62 -1.6 231,318 - leuB MCAODC_01235 0.62 -2.8 231,318 - leuB MCAODC_01235 0.62 -3.6 231,318 - leuB MCAODC_01235 0.62 -1.0 231,318 - leuB MCAODC_01235 0.62 -2.8 231,318 - leuB MCAODC_01235 0.62 +1.4 231,346 + leuB MCAODC_01235 0.64 -5.0 231,482 + leuB MCAODC_01235 0.77 +0.0 231,482 + leuB MCAODC_01235 0.77 -0.1 231,482 + leuB MCAODC_01235 0.77 -1.6 231,482 + leuB MCAODC_01235 0.77 -1.1 231,482 + leuB MCAODC_01235 0.77 -1.9 231,482 + leuB MCAODC_01235 0.77 -3.9 231,483 - leuB MCAODC_01235 0.77 -2.6 231,483 - leuB MCAODC_01235 0.77 -4.3 231,521 - leuB MCAODC_01235 0.80 -2.8 231,634 - -1.0
Or see this region's nucleotide sequence