Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS12300 and ABIE41_RS12305 overlap by 4 nucleotides ABIE41_RS12305 and ABIE41_RS12310 overlap by 1 nucleotides ABIE41_RS12310 and ABIE41_RS12315 are separated by 10 nucleotides ABIE41_RS12315 and ABIE41_RS12320 are separated by 170 nucleotides
ABIE41_RS12300: ABIE41_RS12300 - protein-glutamate O-methyltransferase CheR, at 2,517,816 to 2,518,643
_RS12300
ABIE41_RS12305: ABIE41_RS12305 - chemoreceptor glutamine deamidase CheD, at 2,518,640 to 2,519,233
_RS12305
ABIE41_RS12310: ABIE41_RS12310 - chemotaxis-specific protein-glutamate methyltransferase CheB, at 2,519,233 to 2,520,324
_RS12310
ABIE41_RS12315: ABIE41_RS12315 - response regulator, at 2,520,335 to 2,520,724
_RS12315
ABIE41_RS12320: ABIE41_RS12320 - glutathione peroxidase, at 2,520,895 to 2,521,464
_RS12320
Position (kb)
2519
2520
2521 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2518.296 kb on + strand, within ABIE41_RS12300 at 2518.488 kb on + strand, within ABIE41_RS12300 at 2518.488 kb on + strand, within ABIE41_RS12300 at 2518.489 kb on - strand, within ABIE41_RS12300 at 2518.489 kb on - strand, within ABIE41_RS12300 at 2518.489 kb on - strand, within ABIE41_RS12300 at 2518.489 kb on - strand, within ABIE41_RS12300 at 2518.560 kb on + strand, within ABIE41_RS12300 at 2518.626 kb on + strand at 2518.626 kb on + strand at 2518.626 kb on + strand at 2518.627 kb on - strand at 2518.627 kb on - strand at 2518.667 kb on + strand at 2519.181 kb on - strand at 2519.595 kb on + strand, within ABIE41_RS12310 at 2519.595 kb on + strand, within ABIE41_RS12310 at 2519.595 kb on + strand, within ABIE41_RS12310 at 2519.596 kb on - strand, within ABIE41_RS12310 at 2519.596 kb on - strand, within ABIE41_RS12310 at 2519.853 kb on + strand, within ABIE41_RS12310 at 2520.176 kb on + strand, within ABIE41_RS12310 at 2520.177 kb on - strand, within ABIE41_RS12310 at 2520.322 kb on + strand at 2520.404 kb on + strand, within ABIE41_RS12315 at 2520.405 kb on - strand, within ABIE41_RS12315 at 2520.405 kb on - strand, within ABIE41_RS12315 at 2520.653 kb on + strand, within ABIE41_RS12315 at 2520.653 kb on + strand, within ABIE41_RS12315 at 2520.653 kb on + strand, within ABIE41_RS12315 at 2520.654 kb on - strand, within ABIE41_RS12315 at 2520.654 kb on - strand, within ABIE41_RS12315 at 2520.660 kb on + strand, within ABIE41_RS12315 at 2520.669 kb on - strand, within ABIE41_RS12315 at 2520.669 kb on - strand, within ABIE41_RS12315 at 2520.832 kb on + strand at 2520.832 kb on + strand at 2520.833 kb on - strand at 2520.834 kb on + strand at 2520.834 kb on + strand at 2520.834 kb on + strand at 2520.835 kb on - strand at 2521.249 kb on + strand, within ABIE41_RS12320 at 2521.249 kb on + strand, within ABIE41_RS12320 at 2521.249 kb on + strand, within ABIE41_RS12320 at 2521.250 kb on - strand, within ABIE41_RS12320 at 2521.300 kb on + strand, within ABIE41_RS12320 at 2521.300 kb on + strand, within ABIE41_RS12320 at 2521.300 kb on + strand, within ABIE41_RS12320 at 2521.301 kb on - strand, within ABIE41_RS12320 at 2521.301 kb on - strand, within ABIE41_RS12320 at 2521.301 kb on - strand, within ABIE41_RS12320 at 2521.301 kb on - strand, within ABIE41_RS12320
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 2,518,296 + ABIE41_RS12300 0.58 +0.4 2,518,488 + ABIE41_RS12300 0.81 -0.3 2,518,488 + ABIE41_RS12300 0.81 +0.5 2,518,489 - ABIE41_RS12300 0.81 +0.3 2,518,489 - ABIE41_RS12300 0.81 +0.8 2,518,489 - ABIE41_RS12300 0.81 -1.0 2,518,489 - ABIE41_RS12300 0.81 -0.7 2,518,560 + ABIE41_RS12300 0.90 +1.0 2,518,626 + -0.1 2,518,626 + +1.9 2,518,626 + -0.3 2,518,627 - +0.4 2,518,627 - +1.0 2,518,667 + -1.1 2,519,181 - -2.3 2,519,595 + ABIE41_RS12310 0.33 -1.2 2,519,595 + ABIE41_RS12310 0.33 -1.5 2,519,595 + ABIE41_RS12310 0.33 +0.5 2,519,596 - ABIE41_RS12310 0.33 -1.2 2,519,596 - ABIE41_RS12310 0.33 +0.1 2,519,853 + ABIE41_RS12310 0.57 +1.8 2,520,176 + ABIE41_RS12310 0.86 +0.1 2,520,177 - ABIE41_RS12310 0.86 -0.8 2,520,322 + -1.3 2,520,404 + ABIE41_RS12315 0.18 -0.2 2,520,405 - ABIE41_RS12315 0.18 -0.2 2,520,405 - ABIE41_RS12315 0.18 -0.1 2,520,653 + ABIE41_RS12315 0.82 +0.2 2,520,653 + ABIE41_RS12315 0.82 -1.9 2,520,653 + ABIE41_RS12315 0.82 -0.2 2,520,654 - ABIE41_RS12315 0.82 -0.4 2,520,654 - ABIE41_RS12315 0.82 -0.3 2,520,660 + ABIE41_RS12315 0.83 +0.0 2,520,669 - ABIE41_RS12315 0.86 -1.2 2,520,669 - ABIE41_RS12315 0.86 -1.2 2,520,832 + +0.0 2,520,832 + -0.2 2,520,833 - -0.4 2,520,834 + -1.0 2,520,834 + +1.6 2,520,834 + +0.3 2,520,835 - +0.3 2,521,249 + ABIE41_RS12320 0.62 -0.3 2,521,249 + ABIE41_RS12320 0.62 +0.9 2,521,249 + ABIE41_RS12320 0.62 -3.2 2,521,250 - ABIE41_RS12320 0.62 -0.1 2,521,300 + ABIE41_RS12320 0.71 -0.8 2,521,300 + ABIE41_RS12320 0.71 +0.6 2,521,300 + ABIE41_RS12320 0.71 -1.2 2,521,301 - ABIE41_RS12320 0.71 +0.0 2,521,301 - ABIE41_RS12320 0.71 -0.6 2,521,301 - ABIE41_RS12320 0.71 +1.4 2,521,301 - ABIE41_RS12320 0.71 -0.6
Or see this region's nucleotide sequence