Experiment: Bas06
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mppA and ynaI are separated by 50 nucleotides ynaI and ynaJ are separated by 243 nucleotides ynaJ and uspE are separated by 49 nucleotides
MCAODC_16010: mppA - murein tripeptide ABC transporter substrate-binding protein MppA, at 92,573 to 94,186
mppA
MCAODC_16015: ynaI - low conductance mechanosensitive channel YnaI, at 94,237 to 95,268
ynaI
MCAODC_16020: ynaJ - Uncharacterized protein YnaJ, at 95,512 to 95,769
ynaJ
MCAODC_16025: uspE - universal stress protein UspE, at 95,819 to 96,769
uspE
Position (kb) 94
95
96 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 93.314 kb on - strand, within mppA at 93.389 kb on + strand, within mppA at 93.449 kb on + strand, within mppA at 93.452 kb on + strand, within mppA at 93.514 kb on + strand, within mppA at 93.522 kb on + strand, within mppA at 93.765 kb on + strand, within mppA at 93.893 kb on - strand, within mppA at 94.169 kb on + strand at 94.261 kb on - strand at 94.261 kb on - strand at 94.261 kb on - strand at 94.272 kb on + strand at 94.274 kb on + strand at 94.318 kb on + strand at 94.319 kb on - strand at 94.341 kb on - strand, within ynaI at 94.622 kb on - strand, within ynaI at 94.626 kb on - strand, within ynaI at 94.626 kb on - strand, within ynaI at 95.081 kb on + strand, within ynaI at 95.506 kb on + strand at 95.506 kb on + strand at 95.506 kb on + strand at 95.506 kb on + strand at 95.517 kb on + strand at 95.624 kb on + strand, within ynaJ at 95.624 kb on + strand, within ynaJ at 95.668 kb on + strand, within ynaJ at 95.700 kb on + strand, within ynaJ at 95.700 kb on + strand, within ynaJ at 95.700 kb on + strand, within ynaJ at 95.767 kb on + strand at 95.807 kb on - strand at 95.859 kb on + strand at 95.860 kb on - strand at 95.987 kb on + strand, within uspE at 95.988 kb on - strand, within uspE at 96.119 kb on - strand, within uspE at 96.121 kb on - strand, within uspE at 96.263 kb on - strand, within uspE at 96.263 kb on - strand, within uspE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas06 remove 93,314 - mppA MCAODC_16010 0.46 -2.3 93,389 + mppA MCAODC_16010 0.51 +0.1 93,449 + mppA MCAODC_16010 0.54 -2.8 93,452 + mppA MCAODC_16010 0.54 +0.3 93,514 + mppA MCAODC_16010 0.58 +0.4 93,522 + mppA MCAODC_16010 0.59 -1.2 93,765 + mppA MCAODC_16010 0.74 +2.7 93,893 - mppA MCAODC_16010 0.82 +0.6 94,169 + -0.8 94,261 - +0.9 94,261 - -0.6 94,261 - -0.9 94,272 + -0.9 94,274 + -0.4 94,318 + +0.0 94,319 - -0.6 94,341 - ynaI MCAODC_16015 0.10 -0.7 94,622 - ynaI MCAODC_16015 0.37 -1.2 94,626 - ynaI MCAODC_16015 0.38 +0.3 94,626 - ynaI MCAODC_16015 0.38 -0.1 95,081 + ynaI MCAODC_16015 0.82 -1.3 95,506 + -0.8 95,506 + -0.3 95,506 + -1.3 95,506 + -0.8 95,517 + +1.4 95,624 + ynaJ MCAODC_16020 0.43 +0.5 95,624 + ynaJ MCAODC_16020 0.43 -0.5 95,668 + ynaJ MCAODC_16020 0.60 -0.0 95,700 + ynaJ MCAODC_16020 0.73 -0.1 95,700 + ynaJ MCAODC_16020 0.73 -1.6 95,700 + ynaJ MCAODC_16020 0.73 -0.1 95,767 + -0.4 95,807 - -1.1 95,859 + -0.4 95,860 - +0.4 95,987 + uspE MCAODC_16025 0.18 -0.3 95,988 - uspE MCAODC_16025 0.18 +2.9 96,119 - uspE MCAODC_16025 0.32 -1.6 96,121 - uspE MCAODC_16025 0.32 -2.0 96,263 - uspE MCAODC_16025 0.47 +1.2 96,263 - uspE MCAODC_16025 0.47 -0.1
Or see this region's nucleotide sequence