Strain Fitness in Escherichia coli ECRC101 around MCAODC_14885

Experiment: Bas06

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_14870 and renD overlap by 4 nucleotidesrenD and emrE are separated by 67 nucleotidesemrE and ybcK are separated by 256 nucleotidesybcK and ybcL are separated by 464 nucleotides MCAODC_14870: MCAODC_14870 - replication protein P, at 2,917,406 to 2,917,843 _14870 MCAODC_14875: renD - Putative protein RenD, at 2,917,840 to 2,918,142 renD MCAODC_14880: emrE - multidrug efflux SMR transporter EmrE, at 2,918,210 to 2,918,542 emrE MCAODC_14885: ybcK - Uncharacterized protein YbcK, at 2,918,799 to 2,920,325 ybcK MCAODC_14890: ybcL - Raf kinase inhibitor-like protein YbcL, at 2,920,790 to 2,921,341 ybcL Position (kb) 2918 2919 2920 2921Strain fitness (log2 ratio) -3 -2 -1 0 1at 2918.222 kb on - strandat 2918.422 kb on + strand, within emrEat 2918.427 kb on + strand, within emrEat 2918.474 kb on - strand, within emrEat 2918.476 kb on - strand, within emrEat 2919.805 kb on + strand, within ybcK

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas06
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2,918,222 - -1.5
2,918,422 + emrE MCAODC_14880 0.64 -3.7
2,918,427 + emrE MCAODC_14880 0.65 -0.1
2,918,474 - emrE MCAODC_14880 0.79 -0.8
2,918,476 - emrE MCAODC_14880 0.80 +0.5
2,919,805 + ybcK MCAODC_14885 0.66 -1.2

Or see this region's nucleotide sequence