Strain Fitness in Escherichia coli ECRC101 around MCAODC_00020

Experiment: Bas06

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_00010 and bor are separated by 291 nucleotidesbor and rzpD are separated by 31 nucleotidesrzpD and MCAODC_00025 overlap by 4 nucleotidesMCAODC_00025 and MCAODC_00030 overlap by 14 nucleotides MCAODC_00010: MCAODC_00010 - DUF1398 domain-containing protein, at 763 to 1,173 _00010 MCAODC_00015: bor - serum resistance lipoprotein Bor, at 1,465 to 1,758 bor MCAODC_00020: rzpD - prophage endopeptidase RzpD, at 1,790 to 2,251 rzpD MCAODC_00025: MCAODC_00025 - lysozyme, at 2,248 to 2,724 _00025 MCAODC_00030: MCAODC_00030 - Holin, at 2,711 to 3,016 _00030 Position (kb) 1 2 3Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1.331 kb on - strandat 1.362 kb on + strandat 1.362 kb on + strandat 1.366 kb on - strandat 1.503 kb on + strand, within borat 1.744 kb on + strandat 1.872 kb on + strand, within rzpDat 2.154 kb on + strand, within rzpDat 2.155 kb on - strand, within rzpDat 2.159 kb on - strand, within rzpDat 2.159 kb on - strand, within rzpDat 2.251 kb on + strandat 2.252 kb on - strandat 2.314 kb on - strand, within MCAODC_00025at 2.358 kb on + strand, within MCAODC_00025at 2.407 kb on - strand, within MCAODC_00025at 2.407 kb on - strand, within MCAODC_00025at 2.627 kb on + strand, within MCAODC_00025at 2.812 kb on - strand, within MCAODC_00030at 2.895 kb on - strand, within MCAODC_00030at 2.895 kb on - strand, within MCAODC_00030at 2.930 kb on + strand, within MCAODC_00030at 2.930 kb on + strand, within MCAODC_00030at 3.016 kb on + strandat 3.016 kb on + strandat 3.017 kb on - strandat 3.017 kb on - strandat 3.149 kb on + strandat 3.150 kb on - strandat 3.168 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas06
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1,331 - +0.8
1,362 + -0.1
1,362 + -0.5
1,366 - -0.7
1,503 + bor MCAODC_00015 0.13 +0.8
1,744 + -0.3
1,872 + rzpD MCAODC_00020 0.18 -0.7
2,154 + rzpD MCAODC_00020 0.79 +0.0
2,155 - rzpD MCAODC_00020 0.79 +0.0
2,159 - rzpD MCAODC_00020 0.80 -1.3
2,159 - rzpD MCAODC_00020 0.80 -1.0
2,251 + +1.3
2,252 - +0.8
2,314 - MCAODC_00025 0.14 -0.2
2,358 + MCAODC_00025 0.23 +0.8
2,407 - MCAODC_00025 0.33 +1.8
2,407 - MCAODC_00025 0.33 +0.0
2,627 + MCAODC_00025 0.79 -1.5
2,812 - MCAODC_00030 0.33 +0.3
2,895 - MCAODC_00030 0.60 -0.7
2,895 - MCAODC_00030 0.60 +0.8
2,930 + MCAODC_00030 0.72 +1.1
2,930 + MCAODC_00030 0.72 +0.0
3,016 + +1.4
3,016 + -2.1
3,017 - -2.1
3,017 - +0.2
3,149 + +1.4
3,150 - -2.8
3,168 - -2.1

Or see this region's nucleotide sequence