Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS01335 and ABIE41_RS01340 overlap by 4 nucleotides ABIE41_RS01340 and ABIE41_RS01345 are separated by 32 nucleotides ABIE41_RS01345 and ABIE41_RS01350 are separated by 184 nucleotides ABIE41_RS01350 and ABIE41_RS01355 are separated by 22 nucleotides
ABIE41_RS01335: ABIE41_RS01335 - cell division topological specificity factor MinE, at 265,626 to 265,889
_RS01335
ABIE41_RS01340: ABIE41_RS01340 - septum site-determining protein MinD, at 265,886 to 266,701
_RS01340
ABIE41_RS01345: ABIE41_RS01345 - septum site-determining protein MinC, at 266,734 to 267,468
_RS01345
ABIE41_RS01350: ABIE41_RS01350 - hypothetical protein, at 267,653 to 267,892
_RS01350
ABIE41_RS01355: ABIE41_RS01355 - aldolase/citrate lyase family protein, at 267,915 to 268,712
_RS01355
Position (kb) 266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 265.990 kb on - strand, within ABIE41_RS01340 at 266.161 kb on - strand, within ABIE41_RS01340 at 266.161 kb on - strand, within ABIE41_RS01340 at 266.188 kb on + strand, within ABIE41_RS01340 at 266.188 kb on + strand, within ABIE41_RS01340 at 266.451 kb on + strand, within ABIE41_RS01340 at 266.451 kb on + strand, within ABIE41_RS01340 at 266.451 kb on + strand, within ABIE41_RS01340 at 266.452 kb on - strand, within ABIE41_RS01340 at 266.452 kb on - strand, within ABIE41_RS01340 at 266.452 kb on - strand, within ABIE41_RS01340 at 266.452 kb on - strand, within ABIE41_RS01340 at 266.452 kb on - strand, within ABIE41_RS01340 at 266.452 kb on - strand, within ABIE41_RS01340 at 266.529 kb on + strand, within ABIE41_RS01340 at 266.529 kb on + strand, within ABIE41_RS01340 at 266.529 kb on + strand, within ABIE41_RS01340 at 266.530 kb on - strand, within ABIE41_RS01340 at 266.530 kb on - strand, within ABIE41_RS01340 at 266.701 kb on + strand at 266.701 kb on + strand at 266.701 kb on + strand at 266.702 kb on - strand at 266.702 kb on - strand at 266.702 kb on - strand at 266.702 kb on - strand at 266.717 kb on - strand at 266.828 kb on + strand, within ABIE41_RS01345 at 266.828 kb on + strand, within ABIE41_RS01345 at 266.828 kb on + strand, within ABIE41_RS01345 at 266.829 kb on - strand, within ABIE41_RS01345 at 266.829 kb on - strand, within ABIE41_RS01345 at 266.829 kb on - strand, within ABIE41_RS01345 at 266.933 kb on + strand, within ABIE41_RS01345 at 266.933 kb on + strand, within ABIE41_RS01345 at 267.416 kb on + strand at 267.417 kb on - strand at 267.417 kb on - strand at 267.417 kb on - strand at 267.521 kb on + strand at 267.522 kb on - strand at 267.522 kb on - strand at 267.891 kb on - strand at 268.138 kb on + strand, within ABIE41_RS01355
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 265,990 - ABIE41_RS01340 0.13 -1.4 266,161 - ABIE41_RS01340 0.34 -0.8 266,161 - ABIE41_RS01340 0.34 +0.1 266,188 + ABIE41_RS01340 0.37 +2.6 266,188 + ABIE41_RS01340 0.37 +0.1 266,451 + ABIE41_RS01340 0.69 +0.0 266,451 + ABIE41_RS01340 0.69 +0.2 266,451 + ABIE41_RS01340 0.69 -0.7 266,452 - ABIE41_RS01340 0.69 -0.1 266,452 - ABIE41_RS01340 0.69 +0.2 266,452 - ABIE41_RS01340 0.69 +0.2 266,452 - ABIE41_RS01340 0.69 -1.6 266,452 - ABIE41_RS01340 0.69 -0.7 266,452 - ABIE41_RS01340 0.69 -1.1 266,529 + ABIE41_RS01340 0.79 +0.3 266,529 + ABIE41_RS01340 0.79 +0.1 266,529 + ABIE41_RS01340 0.79 +0.1 266,530 - ABIE41_RS01340 0.79 -1.2 266,530 - ABIE41_RS01340 0.79 +0.5 266,701 + -0.4 266,701 + +0.0 266,701 + +0.2 266,702 - -0.0 266,702 - -0.2 266,702 - -2.9 266,702 - -0.4 266,717 - +0.5 266,828 + ABIE41_RS01345 0.13 -2.5 266,828 + ABIE41_RS01345 0.13 +0.1 266,828 + ABIE41_RS01345 0.13 -1.2 266,829 - ABIE41_RS01345 0.13 -0.5 266,829 - ABIE41_RS01345 0.13 +0.2 266,829 - ABIE41_RS01345 0.13 -0.4 266,933 + ABIE41_RS01345 0.27 +1.3 266,933 + ABIE41_RS01345 0.27 -2.9 267,416 + +0.5 267,417 - -2.2 267,417 - -0.3 267,417 - -0.6 267,521 + -0.1 267,522 - -0.2 267,522 - +0.9 267,891 - +1.0 268,138 + ABIE41_RS01355 0.28 -0.9
Or see this region's nucleotide sequence