Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS14795 and ABIE41_RS14800 are separated by 187 nucleotides ABIE41_RS14800 and ABIE41_RS14805 are separated by 97 nucleotides ABIE41_RS14805 and ABIE41_RS14810 are separated by 121 nucleotides ABIE41_RS14810 and ABIE41_RS14815 are separated by 131 nucleotides
ABIE41_RS14795: ABIE41_RS14795 - magnesium transporter, at 3,024,762 to 3,026,168
_RS14795
ABIE41_RS14800: ABIE41_RS14800 - tRNA-Leu, at 3,026,356 to 3,026,440
_RS14800
ABIE41_RS14805: ABIE41_RS14805 - FliM/FliN family flagellar motor switch protein, at 3,026,538 to 3,026,807
_RS14805
ABIE41_RS14810: ABIE41_RS14810 - lipoyl(octanoyl) transferase LipB, at 3,026,929 to 3,027,624
_RS14810
ABIE41_RS14815: ABIE41_RS14815 - carboxyl transferase domain-containing protein, at 3,027,756 to 3,029,363
_RS14815
Position (kb)
3026
3027 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3025.819 kb on + strand, within ABIE41_RS14795 at 3025.819 kb on + strand, within ABIE41_RS14795 at 3025.819 kb on + strand, within ABIE41_RS14795 at 3025.819 kb on + strand, within ABIE41_RS14795 at 3025.820 kb on - strand, within ABIE41_RS14795 at 3025.820 kb on - strand, within ABIE41_RS14795 at 3025.820 kb on - strand, within ABIE41_RS14795 at 3026.569 kb on + strand, within ABIE41_RS14805 at 3026.569 kb on + strand, within ABIE41_RS14805 at 3026.570 kb on - strand, within ABIE41_RS14805 at 3026.738 kb on - strand, within ABIE41_RS14805 at 3026.738 kb on - strand, within ABIE41_RS14805 at 3026.738 kb on - strand, within ABIE41_RS14805 at 3026.738 kb on - strand, within ABIE41_RS14805 at 3026.738 kb on - strand, within ABIE41_RS14805 at 3026.826 kb on + strand at 3026.826 kb on + strand at 3026.826 kb on + strand at 3026.827 kb on - strand at 3026.827 kb on - strand at 3026.827 kb on - strand at 3027.736 kb on + strand at 3027.737 kb on - strand at 3027.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 3,025,819 + ABIE41_RS14795 0.75 +0.1 3,025,819 + ABIE41_RS14795 0.75 -0.4 3,025,819 + ABIE41_RS14795 0.75 +0.8 3,025,819 + ABIE41_RS14795 0.75 +0.5 3,025,820 - ABIE41_RS14795 0.75 +0.7 3,025,820 - ABIE41_RS14795 0.75 +2.7 3,025,820 - ABIE41_RS14795 0.75 +0.5 3,026,569 + ABIE41_RS14805 0.11 -2.3 3,026,569 + ABIE41_RS14805 0.11 +0.5 3,026,570 - ABIE41_RS14805 0.12 +0.3 3,026,738 - ABIE41_RS14805 0.74 +0.2 3,026,738 - ABIE41_RS14805 0.74 -0.3 3,026,738 - ABIE41_RS14805 0.74 +0.0 3,026,738 - ABIE41_RS14805 0.74 +1.2 3,026,738 - ABIE41_RS14805 0.74 +1.5 3,026,826 + +0.8 3,026,826 + +0.7 3,026,826 + +0.5 3,026,827 - -1.9 3,026,827 - -1.8 3,026,827 - -0.7 3,027,736 + -1.3 3,027,737 - +0.5 3,027,737 - +0.2
Or see this region's nucleotide sequence