Experiment: Ying_all64 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS15215 and ABIE41_RS15220 are separated by 237 nucleotides ABIE41_RS15220 and ABIE41_RS15225 are separated by 31 nucleotides ABIE41_RS15225 and ABIE41_RS15230 are separated by 159 nucleotides ABIE41_RS15230 and ABIE41_RS15235 overlap by 4 nucleotides ABIE41_RS15235 and ABIE41_RS15240 overlap by 14 nucleotides
ABIE41_RS15215: ABIE41_RS15215 - glycerol kinase GlpK, at 3,113,262 to 3,114,779
_RS15215
ABIE41_RS15220: ABIE41_RS15220 - tRNA-Gln, at 3,115,017 to 3,115,090
_RS15220
ABIE41_RS15225: ABIE41_RS15225 - hypothetical protein, at 3,115,122 to 3,115,349
_RS15225
ABIE41_RS15230: ABIE41_RS15230 - glycosyltransferase, at 3,115,509 to 3,116,357
_RS15230
ABIE41_RS15235: ABIE41_RS15235 - SAM-dependent methyltransferase, at 3,116,354 to 3,116,944
_RS15235
ABIE41_RS15240: ABIE41_RS15240 - PIG-L family deacetylase, at 3,116,931 to 3,117,641
_RS15240
Position (kb)
3115
3116
3117 Strain fitness (log2 ratio)
-1
0
1
2 at 3114.618 kb on + strand, within ABIE41_RS15215 at 3114.619 kb on - strand, within ABIE41_RS15215 at 3114.809 kb on - strand at 3114.809 kb on - strand at 3114.869 kb on + strand at 3115.365 kb on - strand at 3115.379 kb on + strand at 3115.379 kb on + strand at 3115.379 kb on + strand at 3115.380 kb on - strand at 3115.380 kb on - strand at 3115.384 kb on - strand at 3115.404 kb on + strand at 3115.404 kb on + strand at 3115.405 kb on - strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.473 kb on - strand at 3115.473 kb on - strand at 3115.514 kb on - strand at 3115.514 kb on - strand at 3115.738 kb on + strand, within ABIE41_RS15230 at 3115.738 kb on + strand, within ABIE41_RS15230 at 3115.739 kb on - strand, within ABIE41_RS15230 at 3115.739 kb on - strand, within ABIE41_RS15230 at 3115.744 kb on + strand, within ABIE41_RS15230 at 3115.744 kb on + strand, within ABIE41_RS15230 at 3115.744 kb on + strand, within ABIE41_RS15230 at 3115.744 kb on + strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3116.197 kb on - strand, within ABIE41_RS15230 at 3116.197 kb on - strand, within ABIE41_RS15230 at 3116.388 kb on + strand at 3116.388 kb on + strand at 3116.479 kb on - strand, within ABIE41_RS15235 at 3116.580 kb on + strand, within ABIE41_RS15235 at 3116.581 kb on - strand, within ABIE41_RS15235 at 3116.581 kb on - strand, within ABIE41_RS15235 at 3116.584 kb on - strand, within ABIE41_RS15235 at 3116.841 kb on + strand, within ABIE41_RS15235 at 3116.841 kb on + strand, within ABIE41_RS15235 at 3116.841 kb on + strand, within ABIE41_RS15235 at 3116.841 kb on + strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.856 kb on + strand, within ABIE41_RS15235 at 3116.913 kb on + strand at 3116.913 kb on + strand at 3116.932 kb on + strand at 3116.932 kb on + strand at 3116.933 kb on - strand at 3116.933 kb on - strand at 3116.962 kb on + strand at 3116.963 kb on - strand at 3117.142 kb on + strand, within ABIE41_RS15240 at 3117.142 kb on + strand, within ABIE41_RS15240 at 3117.143 kb on - strand, within ABIE41_RS15240 at 3117.143 kb on - strand, within ABIE41_RS15240 at 3117.143 kb on - strand, within ABIE41_RS15240 at 3117.143 kb on - strand, within ABIE41_RS15240 at 3117.209 kb on + strand, within ABIE41_RS15240 at 3117.210 kb on - strand, within ABIE41_RS15240
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2 remove 3,114,618 + ABIE41_RS15215 0.89 +0.1 3,114,619 - ABIE41_RS15215 0.89 +0.2 3,114,809 - +0.6 3,114,809 - +0.2 3,114,869 + -0.0 3,115,365 - -0.1 3,115,379 + -0.0 3,115,379 + -0.5 3,115,379 + -0.3 3,115,380 - +1.0 3,115,380 - +0.4 3,115,384 - +0.4 3,115,404 + -0.2 3,115,404 + -0.1 3,115,405 - -0.3 3,115,472 + -0.7 3,115,472 + -0.2 3,115,472 + +1.9 3,115,472 + +2.2 3,115,472 + -0.0 3,115,472 + -1.0 3,115,472 + +0.6 3,115,473 - +0.4 3,115,473 - +0.6 3,115,514 - -0.4 3,115,514 - +0.0 3,115,738 + ABIE41_RS15230 0.27 +0.0 3,115,738 + ABIE41_RS15230 0.27 +0.3 3,115,739 - ABIE41_RS15230 0.27 +0.1 3,115,739 - ABIE41_RS15230 0.27 -0.6 3,115,744 + ABIE41_RS15230 0.28 +0.3 3,115,744 + ABIE41_RS15230 0.28 -0.1 3,115,744 + ABIE41_RS15230 0.28 -1.2 3,115,744 + ABIE41_RS15230 0.28 +0.2 3,115,745 - ABIE41_RS15230 0.28 -0.1 3,115,745 - ABIE41_RS15230 0.28 -0.3 3,115,745 - ABIE41_RS15230 0.28 -0.3 3,115,745 - ABIE41_RS15230 0.28 -0.1 3,115,745 - ABIE41_RS15230 0.28 +0.0 3,116,197 - ABIE41_RS15230 0.81 -0.3 3,116,197 - ABIE41_RS15230 0.81 +0.3 3,116,388 + -0.0 3,116,388 + +0.0 3,116,479 - ABIE41_RS15235 0.21 -0.3 3,116,580 + ABIE41_RS15235 0.38 +0.1 3,116,581 - ABIE41_RS15235 0.38 +0.1 3,116,581 - ABIE41_RS15235 0.38 -0.4 3,116,584 - ABIE41_RS15235 0.39 +0.4 3,116,841 + ABIE41_RS15235 0.82 -0.7 3,116,841 + ABIE41_RS15235 0.82 -1.3 3,116,841 + ABIE41_RS15235 0.82 +1.4 3,116,841 + ABIE41_RS15235 0.82 +0.1 3,116,842 - ABIE41_RS15235 0.83 -0.1 3,116,842 - ABIE41_RS15235 0.83 +1.7 3,116,842 - ABIE41_RS15235 0.83 -0.0 3,116,842 - ABIE41_RS15235 0.83 +0.1 3,116,842 - ABIE41_RS15235 0.83 +0.4 3,116,842 - ABIE41_RS15235 0.83 -0.3 3,116,856 + ABIE41_RS15235 0.85 -0.6 3,116,913 + +1.4 3,116,913 + -0.5 3,116,932 + -0.4 3,116,932 + +1.4 3,116,933 - -1.2 3,116,933 - +0.5 3,116,962 + -0.5 3,116,963 - +0.2 3,117,142 + ABIE41_RS15240 0.30 -0.1 3,117,142 + ABIE41_RS15240 0.30 -0.7 3,117,143 - ABIE41_RS15240 0.30 -0.3 3,117,143 - ABIE41_RS15240 0.30 +0.1 3,117,143 - ABIE41_RS15240 0.30 -0.7 3,117,143 - ABIE41_RS15240 0.30 +0.3 3,117,209 + ABIE41_RS15240 0.39 -0.6 3,117,210 - ABIE41_RS15240 0.39 +1.1
Or see this region's nucleotide sequence