Experiment: Ying_all64 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS05645 and ABIE41_RS05650 are separated by 264 nucleotides ABIE41_RS05650 and ABIE41_RS05655 overlap by 4 nucleotides
ABIE41_RS05645: ABIE41_RS05645 - uroporphyrinogen-III synthase, at 1,159,383 to 1,161,467
_RS05645
ABIE41_RS05650: ABIE41_RS05650 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 1,161,732 to 1,162,802
_RS05650
ABIE41_RS05655: ABIE41_RS05655 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 1,162,799 to 1,163,812
_RS05655
Position (kb)
1161
1162
1163 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1160.735 kb on + strand, within ABIE41_RS05645 at 1160.736 kb on - strand, within ABIE41_RS05645 at 1160.736 kb on - strand, within ABIE41_RS05645 at 1160.736 kb on - strand, within ABIE41_RS05645 at 1160.736 kb on - strand, within ABIE41_RS05645 at 1161.467 kb on + strand at 1161.479 kb on + strand at 1161.653 kb on + strand at 1161.653 kb on + strand at 1161.653 kb on + strand at 1161.653 kb on + strand at 1161.653 kb on + strand at 1161.653 kb on + strand at 1162.851 kb on - strand at 1162.851 kb on - strand at 1163.106 kb on + strand, within ABIE41_RS05655 at 1163.107 kb on - strand, within ABIE41_RS05655 at 1163.330 kb on + strand, within ABIE41_RS05655 at 1163.330 kb on + strand, within ABIE41_RS05655 at 1163.330 kb on + strand, within ABIE41_RS05655 at 1163.330 kb on + strand, within ABIE41_RS05655 at 1163.330 kb on + strand, within ABIE41_RS05655 at 1163.331 kb on - strand, within ABIE41_RS05655 at 1163.331 kb on - strand, within ABIE41_RS05655 at 1163.331 kb on - strand, within ABIE41_RS05655 at 1163.331 kb on - strand, within ABIE41_RS05655 at 1163.421 kb on - strand, within ABIE41_RS05655 at 1163.421 kb on - strand, within ABIE41_RS05655 at 1163.661 kb on + strand, within ABIE41_RS05655 at 1163.661 kb on + strand, within ABIE41_RS05655 at 1163.661 kb on + strand, within ABIE41_RS05655 at 1163.661 kb on + strand, within ABIE41_RS05655 at 1163.661 kb on + strand, within ABIE41_RS05655 at 1163.662 kb on - strand, within ABIE41_RS05655 at 1163.662 kb on - strand, within ABIE41_RS05655 at 1163.727 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2 remove 1,160,735 + ABIE41_RS05645 0.65 +0.4 1,160,736 - ABIE41_RS05645 0.65 -0.3 1,160,736 - ABIE41_RS05645 0.65 -0.2 1,160,736 - ABIE41_RS05645 0.65 +0.9 1,160,736 - ABIE41_RS05645 0.65 -0.2 1,161,467 + +0.7 1,161,479 + -0.5 1,161,653 + -0.4 1,161,653 + +0.3 1,161,653 + -0.1 1,161,653 + +0.5 1,161,653 + -0.1 1,161,653 + -0.5 1,162,851 - -3.3 1,162,851 - -5.0 1,163,106 + ABIE41_RS05655 0.30 -4.8 1,163,107 - ABIE41_RS05655 0.30 -0.6 1,163,330 + ABIE41_RS05655 0.52 -3.6 1,163,330 + ABIE41_RS05655 0.52 -4.8 1,163,330 + ABIE41_RS05655 0.52 -2.5 1,163,330 + ABIE41_RS05655 0.52 -5.7 1,163,330 + ABIE41_RS05655 0.52 -1.6 1,163,331 - ABIE41_RS05655 0.52 -5.7 1,163,331 - ABIE41_RS05655 0.52 -3.6 1,163,331 - ABIE41_RS05655 0.52 -2.4 1,163,331 - ABIE41_RS05655 0.52 -4.3 1,163,421 - ABIE41_RS05655 0.61 -4.0 1,163,421 - ABIE41_RS05655 0.61 -1.2 1,163,661 + ABIE41_RS05655 0.85 -2.2 1,163,661 + ABIE41_RS05655 0.85 -1.4 1,163,661 + ABIE41_RS05655 0.85 -4.1 1,163,661 + ABIE41_RS05655 0.85 -1.4 1,163,661 + ABIE41_RS05655 0.85 -4.5 1,163,662 - ABIE41_RS05655 0.85 -6.1 1,163,662 - ABIE41_RS05655 0.85 -3.9 1,163,727 + -3.2
Or see this region's nucleotide sequence