Strain Fitness in Escherichia coli ECRC101 around MCAODC_26960

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrhsA and MCAODC_26960 are separated by 26 nucleotidesMCAODC_26960 and tnp-ISAs1 are separated by 214 nucleotides MCAODC_26955: rhsA - type IV secretion protein Rhs, at 2,113,258 to 2,114,592 rhsA MCAODC_26960: MCAODC_26960 - Immunity protein of metallopeptidase 4 toxin, at 2,114,619 to 2,114,840 _26960 MCAODC_26965: tnp-ISAs1 - ISAs1 family transposase, at 2,115,055 to 2,116,191 tnp-ISAs1 Position (kb) 2114 2115Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2113.628 kb on - strand, within rhsAat 2113.647 kb on + strand, within rhsAat 2113.684 kb on + strand, within rhsAat 2113.685 kb on - strand, within rhsAat 2113.724 kb on + strand, within rhsAat 2113.837 kb on - strand, within rhsAat 2113.848 kb on + strand, within rhsAat 2113.969 kb on + strand, within rhsAat 2113.970 kb on - strand, within rhsAat 2114.047 kb on + strand, within rhsAat 2114.047 kb on + strand, within rhsAat 2114.047 kb on + strand, within rhsAat 2114.048 kb on - strand, within rhsAat 2114.092 kb on + strand, within rhsAat 2114.098 kb on + strand, within rhsAat 2114.173 kb on + strand, within rhsAat 2114.174 kb on - strand, within rhsAat 2114.174 kb on - strand, within rhsAat 2114.175 kb on + strand, within rhsAat 2114.175 kb on + strand, within rhsAat 2114.238 kb on + strand, within rhsAat 2114.711 kb on + strand, within MCAODC_26960at 2114.723 kb on + strand, within MCAODC_26960at 2114.836 kb on + strandat 2115.039 kb on - strandat 2115.192 kb on + strand, within tnp-ISAs1

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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2,113,628 - rhsA MCAODC_26955 0.28 +0.8
2,113,647 + rhsA MCAODC_26955 0.29 -0.7
2,113,684 + rhsA MCAODC_26955 0.32 +0.3
2,113,685 - rhsA MCAODC_26955 0.32 -1.3
2,113,724 + rhsA MCAODC_26955 0.35 +0.1
2,113,837 - rhsA MCAODC_26955 0.43 -2.6
2,113,848 + rhsA MCAODC_26955 0.44 -1.0
2,113,969 + rhsA MCAODC_26955 0.53 -0.3
2,113,970 - rhsA MCAODC_26955 0.53 -0.3
2,114,047 + rhsA MCAODC_26955 0.59 -0.0
2,114,047 + rhsA MCAODC_26955 0.59 +0.3
2,114,047 + rhsA MCAODC_26955 0.59 -0.4
2,114,048 - rhsA MCAODC_26955 0.59 +0.1
2,114,092 + rhsA MCAODC_26955 0.62 -1.3
2,114,098 + rhsA MCAODC_26955 0.63 -3.0
2,114,173 + rhsA MCAODC_26955 0.69 +0.0
2,114,174 - rhsA MCAODC_26955 0.69 -0.1
2,114,174 - rhsA MCAODC_26955 0.69 +1.5
2,114,175 + rhsA MCAODC_26955 0.69 -0.0
2,114,175 + rhsA MCAODC_26955 0.69 +0.9
2,114,238 + rhsA MCAODC_26955 0.73 -0.5
2,114,711 + MCAODC_26960 0.41 -1.1
2,114,723 + MCAODC_26960 0.47 -0.8
2,114,836 + -0.4
2,115,039 - +0.1
2,115,192 + tnp-ISAs1 MCAODC_26965 0.12 +1.8

Or see this region's nucleotide sequence