Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutB and rutC are separated by 11 nucleotides rutC and rutD are separated by 50 nucleotides rutD and rutE are separated by 9 nucleotides rutE and rutF are separated by 10 nucleotides
MCAODC_24005: rutB - peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB, at 1,529,416 to 1,530,111
rutB
MCAODC_24010: rutC - pyrimidine utilization protein C, at 1,530,123 to 1,530,419
rutC
MCAODC_24015: rutD - pyrimidine utilization protein D, at 1,530,470 to 1,531,270
rutD
MCAODC_24020: rutE - malonic semialdehyde reductase, at 1,531,280 to 1,531,870
rutE
MCAODC_24025: rutF - pyrimidine utilization flavin reductase protein F, at 1,531,881 to 1,532,375
rutF
Position (kb)
1530
1531
1532 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1529.504 kb on - strand, within rutB at 1529.525 kb on + strand, within rutB at 1529.526 kb on - strand, within rutB at 1529.650 kb on + strand, within rutB at 1529.650 kb on + strand, within rutB at 1529.650 kb on + strand, within rutB at 1529.650 kb on + strand, within rutB at 1529.651 kb on - strand, within rutB at 1529.651 kb on - strand, within rutB at 1529.651 kb on - strand, within rutB at 1529.651 kb on - strand, within rutB at 1529.877 kb on - strand, within rutB at 1530.140 kb on + strand at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.208 kb on - strand, within rutC at 1530.208 kb on - strand, within rutC at 1530.208 kb on - strand, within rutC at 1530.208 kb on - strand, within rutC at 1530.242 kb on + strand, within rutC at 1530.243 kb on - strand, within rutC at 1530.307 kb on + strand, within rutC at 1530.360 kb on - strand, within rutC at 1530.411 kb on - strand at 1530.451 kb on - strand at 1530.497 kb on + strand at 1530.514 kb on - strand at 1530.650 kb on + strand, within rutD at 1530.819 kb on + strand, within rutD at 1530.820 kb on - strand, within rutD at 1530.862 kb on - strand, within rutD at 1530.866 kb on + strand, within rutD at 1531.003 kb on - strand, within rutD at 1531.016 kb on - strand, within rutD at 1531.211 kb on - strand at 1531.245 kb on + strand at 1531.245 kb on + strand at 1531.246 kb on - strand at 1531.246 kb on - strand at 1531.246 kb on - strand at 1531.246 kb on - strand at 1531.279 kb on + strand at 1531.280 kb on - strand at 1531.280 kb on - strand at 1531.421 kb on - strand, within rutE at 1531.421 kb on - strand, within rutE at 1531.421 kb on - strand, within rutE at 1531.421 kb on - strand, within rutE at 1531.421 kb on - strand, within rutE at 1531.438 kb on + strand, within rutE at 1531.438 kb on + strand, within rutE at 1531.740 kb on + strand, within rutE at 1531.741 kb on - strand, within rutE at 1531.789 kb on + strand, within rutE at 1531.789 kb on + strand, within rutE at 1531.792 kb on + strand, within rutE at 1531.868 kb on + strand at 1531.869 kb on - strand at 1531.881 kb on - strand at 1531.881 kb on - strand at 1531.881 kb on - strand at 1531.881 kb on - strand at 1532.092 kb on + strand, within rutF at 1532.093 kb on - strand, within rutF at 1532.096 kb on + strand, within rutF at 1532.096 kb on + strand, within rutF
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,529,504 - rutB MCAODC_24005 0.13 +0.4 1,529,525 + rutB MCAODC_24005 0.16 +0.2 1,529,526 - rutB MCAODC_24005 0.16 +0.2 1,529,650 + rutB MCAODC_24005 0.34 -2.5 1,529,650 + rutB MCAODC_24005 0.34 -0.9 1,529,650 + rutB MCAODC_24005 0.34 +0.0 1,529,650 + rutB MCAODC_24005 0.34 +1.0 1,529,651 - rutB MCAODC_24005 0.34 +0.7 1,529,651 - rutB MCAODC_24005 0.34 +0.9 1,529,651 - rutB MCAODC_24005 0.34 +0.7 1,529,651 - rutB MCAODC_24005 0.34 +0.4 1,529,877 - rutB MCAODC_24005 0.66 -0.3 1,530,140 + +0.1 1,530,207 + rutC MCAODC_24010 0.28 -1.2 1,530,207 + rutC MCAODC_24010 0.28 +0.3 1,530,207 + rutC MCAODC_24010 0.28 -0.9 1,530,207 + rutC MCAODC_24010 0.28 +5.3 1,530,207 + rutC MCAODC_24010 0.28 +1.0 1,530,207 + rutC MCAODC_24010 0.28 +0.1 1,530,207 + rutC MCAODC_24010 0.28 -0.3 1,530,207 + rutC MCAODC_24010 0.28 +0.7 1,530,207 + rutC MCAODC_24010 0.28 -0.7 1,530,207 + rutC MCAODC_24010 0.28 +0.7 1,530,207 + rutC MCAODC_24010 0.28 -0.7 1,530,208 - rutC MCAODC_24010 0.29 +0.4 1,530,208 - rutC MCAODC_24010 0.29 -0.1 1,530,208 - rutC MCAODC_24010 0.29 +0.6 1,530,208 - rutC MCAODC_24010 0.29 -0.5 1,530,242 + rutC MCAODC_24010 0.40 +0.1 1,530,243 - rutC MCAODC_24010 0.40 +1.3 1,530,307 + rutC MCAODC_24010 0.62 +0.9 1,530,360 - rutC MCAODC_24010 0.80 -0.7 1,530,411 - +0.0 1,530,451 - -1.0 1,530,497 + +0.8 1,530,514 - +0.3 1,530,650 + rutD MCAODC_24015 0.22 +3.0 1,530,819 + rutD MCAODC_24015 0.44 -2.2 1,530,820 - rutD MCAODC_24015 0.44 +1.4 1,530,862 - rutD MCAODC_24015 0.49 -1.2 1,530,866 + rutD MCAODC_24015 0.49 -2.1 1,531,003 - rutD MCAODC_24015 0.67 -1.4 1,531,016 - rutD MCAODC_24015 0.68 -2.7 1,531,211 - -3.2 1,531,245 + -0.8 1,531,245 + +0.2 1,531,246 - -0.9 1,531,246 - -1.2 1,531,246 - +0.1 1,531,246 - -0.7 1,531,279 + -2.9 1,531,280 - +0.8 1,531,280 - +0.7 1,531,421 - rutE MCAODC_24020 0.24 -0.5 1,531,421 - rutE MCAODC_24020 0.24 -0.9 1,531,421 - rutE MCAODC_24020 0.24 -1.2 1,531,421 - rutE MCAODC_24020 0.24 +0.4 1,531,421 - rutE MCAODC_24020 0.24 +0.1 1,531,438 + rutE MCAODC_24020 0.27 -0.7 1,531,438 + rutE MCAODC_24020 0.27 -0.0 1,531,740 + rutE MCAODC_24020 0.78 +0.6 1,531,741 - rutE MCAODC_24020 0.78 +0.1 1,531,789 + rutE MCAODC_24020 0.86 +0.4 1,531,789 + rutE MCAODC_24020 0.86 +0.1 1,531,792 + rutE MCAODC_24020 0.87 -0.5 1,531,868 + -1.6 1,531,869 - -0.8 1,531,881 - -2.7 1,531,881 - +3.3 1,531,881 - +0.3 1,531,881 - -1.3 1,532,092 + rutF MCAODC_24025 0.43 -0.5 1,532,093 - rutF MCAODC_24025 0.43 +0.6 1,532,096 + rutF MCAODC_24025 0.43 +1.5 1,532,096 + rutF MCAODC_24025 0.43 +0.4
Or see this region's nucleotide sequence