Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_22395 and MCAODC_22400 are separated by 126 nucleotides MCAODC_22400 and ssb are separated by 10 nucleotides ssb and MCAODC_22410 overlap by 4 nucleotides MCAODC_22410 and MCAODC_22415 are separated by 200 nucleotides
MCAODC_22395: MCAODC_22395 - hypothetical protein, at 1,241,531 to 1,241,755
_22395
MCAODC_22400: MCAODC_22400 - PerC family transcriptional regulator, at 1,241,882 to 1,242,154
_22400
MCAODC_22405: ssb - single-stranded DNA-binding protein, at 1,242,165 to 1,242,575
ssb
MCAODC_22410: MCAODC_22410 - hypothetical protein, at 1,242,572 to 1,242,823
_22410
MCAODC_22415: MCAODC_22415 - DNA primase, at 1,243,024 to 1,244,424
_22415
Position (kb)
1242
1243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1241.717 kb on - strand, within MCAODC_22395 at 1241.717 kb on - strand, within MCAODC_22395 at 1241.730 kb on - strand, within MCAODC_22395 at 1241.733 kb on - strand at 1241.792 kb on - strand at 1241.795 kb on - strand at 1241.795 kb on - strand at 1241.798 kb on + strand at 1241.799 kb on - strand at 1241.799 kb on - strand at 1241.799 kb on - strand at 1241.884 kb on - strand at 1241.906 kb on + strand at 1241.907 kb on - strand at 1241.907 kb on - strand at 1241.928 kb on - strand, within MCAODC_22400 at 1241.928 kb on - strand, within MCAODC_22400 at 1241.928 kb on - strand, within MCAODC_22400 at 1241.928 kb on - strand, within MCAODC_22400 at 1241.928 kb on - strand, within MCAODC_22400 at 1241.943 kb on + strand, within MCAODC_22400 at 1241.944 kb on - strand, within MCAODC_22400 at 1241.944 kb on - strand, within MCAODC_22400 at 1242.109 kb on - strand, within MCAODC_22400 at 1242.109 kb on - strand, within MCAODC_22400 at 1242.149 kb on + strand at 1242.150 kb on - strand at 1242.150 kb on - strand at 1242.177 kb on - strand at 1242.177 kb on - strand at 1242.279 kb on + strand, within ssb at 1242.279 kb on + strand, within ssb at 1242.611 kb on + strand, within MCAODC_22410 at 1242.686 kb on + strand, within MCAODC_22410 at 1242.787 kb on - strand, within MCAODC_22410 at 1242.901 kb on - strand at 1242.948 kb on + strand at 1242.948 kb on + strand at 1242.949 kb on - strand at 1242.949 kb on - strand at 1242.950 kb on + strand at 1243.037 kb on - strand at 1243.037 kb on - strand at 1243.775 kb on - strand, within MCAODC_22415 at 1243.793 kb on - strand, within MCAODC_22415 at 1243.793 kb on - strand, within MCAODC_22415 at 1243.793 kb on - strand, within MCAODC_22415 at 1243.793 kb on - strand, within MCAODC_22415 at 1243.815 kb on - strand, within MCAODC_22415
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,241,717 - MCAODC_22395 0.83 +2.7 1,241,717 - MCAODC_22395 0.83 -0.4 1,241,730 - MCAODC_22395 0.88 -0.9 1,241,733 - -0.1 1,241,792 - -0.5 1,241,795 - +0.6 1,241,795 - -1.6 1,241,798 + -0.4 1,241,799 - -0.4 1,241,799 - -0.9 1,241,799 - +0.5 1,241,884 - -1.8 1,241,906 + +1.2 1,241,907 - +0.8 1,241,907 - -0.3 1,241,928 - MCAODC_22400 0.17 -2.3 1,241,928 - MCAODC_22400 0.17 -2.2 1,241,928 - MCAODC_22400 0.17 -0.5 1,241,928 - MCAODC_22400 0.17 -0.7 1,241,928 - MCAODC_22400 0.17 -1.5 1,241,943 + MCAODC_22400 0.22 +0.4 1,241,944 - MCAODC_22400 0.23 +1.6 1,241,944 - MCAODC_22400 0.23 -0.6 1,242,109 - MCAODC_22400 0.83 -0.2 1,242,109 - MCAODC_22400 0.83 +0.3 1,242,149 + +1.6 1,242,150 - -1.7 1,242,150 - -0.2 1,242,177 - +0.4 1,242,177 - +1.5 1,242,279 + ssb MCAODC_22405 0.28 -0.8 1,242,279 + ssb MCAODC_22405 0.28 +0.3 1,242,611 + MCAODC_22410 0.15 -2.8 1,242,686 + MCAODC_22410 0.45 -1.6 1,242,787 - MCAODC_22410 0.85 -1.8 1,242,901 - -0.7 1,242,948 + -1.2 1,242,948 + +0.2 1,242,949 - -2.1 1,242,949 - -0.6 1,242,950 + +1.0 1,243,037 - +0.7 1,243,037 - +0.1 1,243,775 - MCAODC_22415 0.54 -0.3 1,243,793 - MCAODC_22415 0.55 -0.6 1,243,793 - MCAODC_22415 0.55 -1.7 1,243,793 - MCAODC_22415 0.55 -2.3 1,243,793 - MCAODC_22415 0.55 -0.8 1,243,815 - MCAODC_22415 0.56 -2.1
Or see this region's nucleotide sequence