Strain Fitness in Escherichia coli ECRC101 around MCAODC_17280

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnleG7 and MCAODC_17275 are separated by 273 nucleotidesMCAODC_17275 and nleG2-4 are separated by 290 nucleotidesnleG2-4 and MCAODC_17285 are separated by 113 nucleotidesMCAODC_17285 and MCAODC_17290 are separated by 1 nucleotides MCAODC_17270: nleG7 - Type III secretion system effector NleG7, E3 Ubiquitin Ligase, at 339,204 to 339,854 nleG7 MCAODC_17275: MCAODC_17275 - T3SS effector NleG, at 340,128 to 340,274 _17275 MCAODC_17280: nleG2-4 - DUF1076 domain-containing protein, at 340,565 to 341,140 nleG2-4 MCAODC_17285: MCAODC_17285 - phage tail protein, at 341,254 to 341,523 _17285 MCAODC_17290: MCAODC_17290 - phage tail protein, at 341,525 to 342,748 _17290 Position (kb) 340 341 342Strain fitness (log2 ratio) -2 -1 0 1at 339.589 kb on - strand, within nleG7at 339.847 kb on - strandat 339.869 kb on - strandat 339.917 kb on - strandat 339.917 kb on - strandat 339.952 kb on - strandat 340.070 kb on - strandat 340.254 kb on - strand, within MCAODC_17275at 340.255 kb on + strand, within MCAODC_17275at 340.255 kb on + strand, within MCAODC_17275at 340.255 kb on + strand, within MCAODC_17275at 340.280 kb on + strandat 340.524 kb on - strandat 340.543 kb on + strandat 340.939 kb on + strand, within nleG2-4at 340.949 kb on - strand, within nleG2-4at 340.953 kb on - strand, within nleG2-4at 341.016 kb on + strand, within nleG2-4at 341.016 kb on + strand, within nleG2-4at 341.016 kb on + strand, within nleG2-4at 341.016 kb on + strand, within nleG2-4at 341.158 kb on - strandat 341.161 kb on + strandat 341.203 kb on - strandat 341.203 kb on - strandat 341.256 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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339,589 - nleG7 MCAODC_17270 0.59 -1.4
339,847 - +1.1
339,869 - -2.0
339,917 - -1.3
339,917 - -0.6
339,952 - -1.8
340,070 - +1.2
340,254 - MCAODC_17275 0.86 -0.6
340,255 + MCAODC_17275 0.86 +0.4
340,255 + MCAODC_17275 0.86 +1.4
340,255 + MCAODC_17275 0.86 -1.0
340,280 + +1.3
340,524 - -1.8
340,543 + -0.8
340,939 + nleG2-4 MCAODC_17280 0.65 -0.9
340,949 - nleG2-4 MCAODC_17280 0.67 -0.8
340,953 - nleG2-4 MCAODC_17280 0.67 -1.3
341,016 + nleG2-4 MCAODC_17280 0.78 -0.0
341,016 + nleG2-4 MCAODC_17280 0.78 -1.2
341,016 + nleG2-4 MCAODC_17280 0.78 -0.1
341,016 + nleG2-4 MCAODC_17280 0.78 -1.1
341,158 - -2.4
341,161 + -1.4
341,203 - +0.4
341,203 - -2.6
341,256 - +1.0

Or see this region's nucleotide sequence