Strain Fitness in Escherichia coli ECRC101 around MCAODC_01515

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntespX1 and fimA are separated by 301 nucleotidesfimA and fimC are separated by 49 nucleotidesfimC and fimD are separated by 12 nucleotides MCAODC_01510: espX1 - type III secretion system effector EspX1, at 292,440 to 293,861 espX1 MCAODC_01515: fimA - fimbrial protein, at 294,163 to 294,696 fimA MCAODC_01520: fimC - molecular chaperone, at 294,746 to 295,429 fimC MCAODC_01525: fimD - fimbrial assembly protein, at 295,442 to 297,343 fimD Position (kb) 294 295Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 293.369 kb on - strand, within espX1at 293.425 kb on + strand, within espX1at 293.518 kb on + strand, within espX1at 293.541 kb on - strand, within espX1at 293.614 kb on + strand, within espX1at 293.708 kb on - strand, within espX1at 293.965 kb on + strandat 294.001 kb on + strandat 294.255 kb on + strand, within fimAat 294.471 kb on + strand, within fimAat 294.579 kb on + strand, within fimAat 294.619 kb on + strand, within fimAat 294.660 kb on - strandat 294.660 kb on - strandat 294.678 kb on + strandat 294.757 kb on - strandat 295.340 kb on - strand, within fimCat 295.602 kb on - strandat 295.602 kb on - strandat 295.688 kb on - strand, within fimD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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293,369 - espX1 MCAODC_01510 0.65 -2.6
293,425 + espX1 MCAODC_01510 0.69 -0.2
293,518 + espX1 MCAODC_01510 0.76 -2.4
293,541 - espX1 MCAODC_01510 0.77 -1.4
293,614 + espX1 MCAODC_01510 0.83 +0.0
293,708 - espX1 MCAODC_01510 0.89 +0.7
293,965 + +1.4
294,001 + +0.9
294,255 + fimA MCAODC_01515 0.17 -2.2
294,471 + fimA MCAODC_01515 0.58 -2.2
294,579 + fimA MCAODC_01515 0.78 +0.0
294,619 + fimA MCAODC_01515 0.85 -3.3
294,660 - -1.1
294,660 - -1.5
294,678 + +0.0
294,757 - -2.1
295,340 - fimC MCAODC_01520 0.87 -1.4
295,602 - +0.3
295,602 - +0.5
295,688 - fimD MCAODC_01525 0.13 +2.5

Or see this region's nucleotide sequence