Experiment: Ying_all64 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS11295 and ABIE41_RS11300 are separated by 9 nucleotides ABIE41_RS11300 and ABIE41_RS11305 overlap by 11 nucleotides ABIE41_RS11305 and ABIE41_RS11310 are separated by 144 nucleotides ABIE41_RS11310 and ABIE41_RS11315 are separated by 162 nucleotides ABIE41_RS11315 and ABIE41_RS11320 overlap by 4 nucleotides
ABIE41_RS11295: ABIE41_RS11295 - redox-regulated ATPase YchF, at 2,322,939 to 2,324,036
_RS11295
ABIE41_RS11300: ABIE41_RS11300 - PIN domain-containing protein, at 2,324,046 to 2,324,492
_RS11300
ABIE41_RS11305: ABIE41_RS11305 - hypothetical protein, at 2,324,482 to 2,324,712
_RS11305
ABIE41_RS11310: ABIE41_RS11310 - hypothetical protein, at 2,324,857 to 2,325,453
_RS11310
ABIE41_RS11315: ABIE41_RS11315 - pyridoxamine 5'-phosphate oxidase family protein, at 2,325,616 to 2,326,050
_RS11315
ABIE41_RS11320: ABIE41_RS11320 - aminoacyl-tRNA hydrolase, at 2,326,047 to 2,326,658
_RS11320
Position (kb)
2324
2325
2326 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2324.130 kb on - strand, within ABIE41_RS11300 at 2324.130 kb on - strand, within ABIE41_RS11300 at 2324.146 kb on + strand, within ABIE41_RS11300 at 2324.146 kb on + strand, within ABIE41_RS11300 at 2324.201 kb on - strand, within ABIE41_RS11300 at 2324.237 kb on + strand, within ABIE41_RS11300 at 2324.237 kb on + strand, within ABIE41_RS11300 at 2324.266 kb on + strand, within ABIE41_RS11300 at 2324.355 kb on + strand, within ABIE41_RS11300 at 2324.356 kb on - strand, within ABIE41_RS11300 at 2324.429 kb on + strand, within ABIE41_RS11300 at 2324.429 kb on + strand, within ABIE41_RS11300 at 2324.429 kb on + strand, within ABIE41_RS11300 at 2324.430 kb on - strand, within ABIE41_RS11300 at 2324.430 kb on - strand, within ABIE41_RS11300 at 2324.452 kb on + strand at 2324.453 kb on - strand at 2324.483 kb on + strand at 2324.492 kb on + strand at 2324.493 kb on - strand at 2324.493 kb on - strand at 2324.493 kb on - strand at 2324.624 kb on + strand, within ABIE41_RS11305 at 2324.625 kb on - strand, within ABIE41_RS11305 at 2324.633 kb on - strand, within ABIE41_RS11305 at 2324.931 kb on + strand, within ABIE41_RS11310 at 2324.931 kb on + strand, within ABIE41_RS11310 at 2324.931 kb on + strand, within ABIE41_RS11310 at 2324.932 kb on - strand, within ABIE41_RS11310 at 2325.023 kb on - strand, within ABIE41_RS11310 at 2325.023 kb on - strand, within ABIE41_RS11310 at 2325.269 kb on - strand, within ABIE41_RS11310 at 2325.447 kb on + strand at 2325.449 kb on - strand at 2325.641 kb on + strand at 2325.641 kb on + strand at 2325.642 kb on - strand at 2325.642 kb on - strand at 2325.705 kb on - strand, within ABIE41_RS11315 at 2325.743 kb on - strand, within ABIE41_RS11315 at 2325.870 kb on + strand, within ABIE41_RS11315 at 2325.871 kb on - strand, within ABIE41_RS11315 at 2325.871 kb on - strand, within ABIE41_RS11315 at 2326.048 kb on + strand at 2326.048 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2 remove 2,324,130 - ABIE41_RS11300 0.19 +0.2 2,324,130 - ABIE41_RS11300 0.19 -0.8 2,324,146 + ABIE41_RS11300 0.22 -2.8 2,324,146 + ABIE41_RS11300 0.22 +0.3 2,324,201 - ABIE41_RS11300 0.35 -0.5 2,324,237 + ABIE41_RS11300 0.43 -0.6 2,324,237 + ABIE41_RS11300 0.43 -0.3 2,324,266 + ABIE41_RS11300 0.49 -0.1 2,324,355 + ABIE41_RS11300 0.69 -0.1 2,324,356 - ABIE41_RS11300 0.69 +0.2 2,324,429 + ABIE41_RS11300 0.86 +0.0 2,324,429 + ABIE41_RS11300 0.86 +0.0 2,324,429 + ABIE41_RS11300 0.86 +0.6 2,324,430 - ABIE41_RS11300 0.86 -0.1 2,324,430 - ABIE41_RS11300 0.86 +0.0 2,324,452 + -0.3 2,324,453 - -1.2 2,324,483 + +0.4 2,324,492 + -0.4 2,324,493 - +0.7 2,324,493 - +0.3 2,324,493 - +0.6 2,324,624 + ABIE41_RS11305 0.61 +0.4 2,324,625 - ABIE41_RS11305 0.62 +0.3 2,324,633 - ABIE41_RS11305 0.65 +0.1 2,324,931 + ABIE41_RS11310 0.12 -0.1 2,324,931 + ABIE41_RS11310 0.12 +0.6 2,324,931 + ABIE41_RS11310 0.12 -0.4 2,324,932 - ABIE41_RS11310 0.13 -1.5 2,325,023 - ABIE41_RS11310 0.28 -1.4 2,325,023 - ABIE41_RS11310 0.28 +1.4 2,325,269 - ABIE41_RS11310 0.69 -0.8 2,325,447 + +0.3 2,325,449 - -0.9 2,325,641 + -0.9 2,325,641 + -0.3 2,325,642 - +0.1 2,325,642 - -0.1 2,325,705 - ABIE41_RS11315 0.20 +0.7 2,325,743 - ABIE41_RS11315 0.29 +0.3 2,325,870 + ABIE41_RS11315 0.58 +0.0 2,325,871 - ABIE41_RS11315 0.59 -0.4 2,325,871 - ABIE41_RS11315 0.59 -0.7 2,326,048 + -0.2 2,326,048 + +0.2
Or see this region's nucleotide sequence