Strain Fitness in Escherichia coli ECRC101 around MCAODC_12270

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisS and yfgM are separated by 17 nucleotidesyfgM and bamB are separated by 10 nucleotidesbamB and der are separated by 117 nucleotides MCAODC_12260: hisS - histidine--tRNA ligase, at 2,428,249 to 2,429,523 hisS MCAODC_12265: yfgM - ancillary SecYEG translocon subunit YfgM, at 2,429,541 to 2,430,161 yfgM MCAODC_12270: bamB - outer membrane protein assembly factor BamB, at 2,430,172 to 2,431,350 bamB MCAODC_12275: der - ribosome biogenesis GTPase Der, at 2,431,468 to 2,432,940 der Position (kb) 2430 2431 2432Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2429.521 kb on + strandat 2429.550 kb on + strandat 2429.796 kb on + strand, within yfgMat 2429.796 kb on + strand, within yfgMat 2429.796 kb on + strand, within yfgMat 2429.796 kb on + strand, within yfgMat 2429.796 kb on + strand, within yfgMat 2429.796 kb on + strand, within yfgMat 2429.796 kb on + strand, within yfgMat 2429.987 kb on + strand, within yfgMat 2430.107 kb on + strandat 2430.538 kb on + strand, within bamBat 2430.538 kb on + strand, within bamBat 2430.538 kb on + strand, within bamBat 2430.606 kb on + strand, within bamBat 2430.606 kb on + strand, within bamBat 2430.606 kb on + strand, within bamBat 2430.864 kb on + strand, within bamBat 2430.864 kb on + strand, within bamBat 2430.864 kb on + strand, within bamBat 2430.958 kb on + strand, within bamBat 2430.959 kb on - strand, within bamBat 2430.960 kb on + strand, within bamBat 2430.960 kb on + strand, within bamBat 2430.960 kb on + strand, within bamBat 2431.002 kb on + strand, within bamBat 2431.060 kb on + strand, within bamBat 2431.060 kb on + strand, within bamBat 2431.168 kb on + strand, within bamBat 2431.168 kb on + strand, within bamBat 2431.170 kb on + strand, within bamBat 2431.170 kb on + strand, within bamBat 2431.260 kb on + strandat 2431.338 kb on + strandat 2431.338 kb on + strandat 2431.341 kb on + strandat 2431.341 kb on + strandat 2431.425 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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2,429,521 + +1.5
2,429,550 + +1.4
2,429,796 + yfgM MCAODC_12265 0.41 -0.3
2,429,796 + yfgM MCAODC_12265 0.41 +0.5
2,429,796 + yfgM MCAODC_12265 0.41 -0.8
2,429,796 + yfgM MCAODC_12265 0.41 -0.2
2,429,796 + yfgM MCAODC_12265 0.41 -0.1
2,429,796 + yfgM MCAODC_12265 0.41 -2.8
2,429,796 + yfgM MCAODC_12265 0.41 +1.4
2,429,987 + yfgM MCAODC_12265 0.72 +1.0
2,430,107 + -1.3
2,430,538 + bamB MCAODC_12270 0.31 -1.8
2,430,538 + bamB MCAODC_12270 0.31 -0.9
2,430,538 + bamB MCAODC_12270 0.31 -1.8
2,430,606 + bamB MCAODC_12270 0.37 -1.0
2,430,606 + bamB MCAODC_12270 0.37 -2.4
2,430,606 + bamB MCAODC_12270 0.37 +0.0
2,430,864 + bamB MCAODC_12270 0.59 -1.2
2,430,864 + bamB MCAODC_12270 0.59 -0.4
2,430,864 + bamB MCAODC_12270 0.59 -1.1
2,430,958 + bamB MCAODC_12270 0.67 +2.0
2,430,959 - bamB MCAODC_12270 0.67 -1.5
2,430,960 + bamB MCAODC_12270 0.67 -1.5
2,430,960 + bamB MCAODC_12270 0.67 +0.6
2,430,960 + bamB MCAODC_12270 0.67 +0.8
2,431,002 + bamB MCAODC_12270 0.70 -1.0
2,431,060 + bamB MCAODC_12270 0.75 -0.9
2,431,060 + bamB MCAODC_12270 0.75 -1.2
2,431,168 + bamB MCAODC_12270 0.84 -0.1
2,431,168 + bamB MCAODC_12270 0.84 +0.6
2,431,170 + bamB MCAODC_12270 0.85 -1.3
2,431,170 + bamB MCAODC_12270 0.85 -0.1
2,431,260 + -0.7
2,431,338 + +0.7
2,431,338 + -0.1
2,431,341 + +2.6
2,431,341 + +0.6
2,431,425 + -0.1

Or see this region's nucleotide sequence