Strain Fitness in Escherichia coli ECRC101 around MCAODC_16800

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuxaB and gGDEF are separated by 226 nucleotidesgGDEF and yneG are separated by 138 nucleotidesyneG and glsB overlap by 1 nucleotides MCAODC_16795: uxaB - tagaturonate reductase, at 270,715 to 272,166 uxaB MCAODC_16800: gGDEF - diguanylate cyclase, at 272,393 to 273,811 gGDEF MCAODC_16805: yneG - Uncharacterized protein YneG, at 273,950 to 274,309 yneG MCAODC_16810: glsB - glutaminase B, at 274,309 to 275,235 glsB Position (kb) 272 273 274Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 271.434 kb on - strand, within uxaBat 271.492 kb on - strand, within uxaBat 271.576 kb on - strand, within uxaBat 271.643 kb on + strand, within uxaBat 272.115 kb on + strandat 272.546 kb on + strand, within gGDEFat 272.767 kb on - strand, within gGDEFat 272.767 kb on - strand, within gGDEFat 272.767 kb on - strand, within gGDEFat 272.767 kb on - strand, within gGDEFat 272.791 kb on - strand, within gGDEFat 272.859 kb on + strand, within gGDEFat 273.058 kb on + strand, within gGDEFat 273.283 kb on + strand, within gGDEFat 273.284 kb on - strand, within gGDEFat 273.284 kb on - strand, within gGDEFat 273.359 kb on - strand, within gGDEFat 273.399 kb on - strand, within gGDEFat 273.402 kb on - strand, within gGDEFat 273.420 kb on + strand, within gGDEFat 273.424 kb on + strand, within gGDEFat 273.424 kb on + strand, within gGDEFat 273.444 kb on - strand, within gGDEFat 273.606 kb on + strand, within gGDEFat 273.606 kb on + strand, within gGDEFat 273.755 kb on - strandat 273.755 kb on - strandat 273.813 kb on - strandat 273.856 kb on - strandat 273.856 kb on - strandat 273.856 kb on - strandat 273.856 kb on - strandat 273.968 kb on + strandat 274.000 kb on + strand, within yneGat 274.002 kb on + strand, within yneGat 274.003 kb on - strand, within yneGat 274.045 kb on + strand, within yneGat 274.225 kb on - strand, within yneGat 274.510 kb on - strand, within glsBat 274.576 kb on - strand, within glsBat 274.786 kb on - strand, within glsB

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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271,434 - uxaB MCAODC_16795 0.50 -0.8
271,492 - uxaB MCAODC_16795 0.54 -0.0
271,576 - uxaB MCAODC_16795 0.59 +0.7
271,643 + uxaB MCAODC_16795 0.64 -0.7
272,115 + -1.2
272,546 + gGDEF MCAODC_16800 0.11 +0.9
272,767 - gGDEF MCAODC_16800 0.26 +2.8
272,767 - gGDEF MCAODC_16800 0.26 +1.4
272,767 - gGDEF MCAODC_16800 0.26 +2.0
272,767 - gGDEF MCAODC_16800 0.26 +1.2
272,791 - gGDEF MCAODC_16800 0.28 +4.4
272,859 + gGDEF MCAODC_16800 0.33 +1.6
273,058 + gGDEF MCAODC_16800 0.47 -0.9
273,283 + gGDEF MCAODC_16800 0.63 +0.8
273,284 - gGDEF MCAODC_16800 0.63 +2.3
273,284 - gGDEF MCAODC_16800 0.63 +2.8
273,359 - gGDEF MCAODC_16800 0.68 +1.8
273,399 - gGDEF MCAODC_16800 0.71 +1.0
273,402 - gGDEF MCAODC_16800 0.71 +1.0
273,420 + gGDEF MCAODC_16800 0.72 +0.7
273,424 + gGDEF MCAODC_16800 0.73 +1.7
273,424 + gGDEF MCAODC_16800 0.73 +1.7
273,444 - gGDEF MCAODC_16800 0.74 -0.0
273,606 + gGDEF MCAODC_16800 0.85 +1.8
273,606 + gGDEF MCAODC_16800 0.85 +2.1
273,755 - +0.6
273,755 - +2.2
273,813 - +1.9
273,856 - -2.2
273,856 - +0.2
273,856 - -0.3
273,856 - -0.4
273,968 + -0.0
274,000 + yneG MCAODC_16805 0.14 +0.8
274,002 + yneG MCAODC_16805 0.14 +0.2
274,003 - yneG MCAODC_16805 0.15 -0.8
274,045 + yneG MCAODC_16805 0.26 +0.8
274,225 - yneG MCAODC_16805 0.76 -2.2
274,510 - glsB MCAODC_16810 0.22 -0.3
274,576 - glsB MCAODC_16810 0.29 +1.8
274,786 - glsB MCAODC_16810 0.51 +0.2

Or see this region's nucleotide sequence