Experiment: T2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt holB and tmk overlap by 4 nucleotides tmk and yceG overlap by 11 nucleotides yceG and pabC are separated by 2 nucleotides pabC and fabF are separated by 119 nucleotides
MCAODC_22905: holB - DNA polymerase III subunit delta', at 1,324,618 to 1,325,622
holB
MCAODC_22910: tmk - dTMP kinase, at 1,325,619 to 1,326,260
tmk
MCAODC_22915: yceG - cell division protein YceG, at 1,326,250 to 1,327,272
yceG
MCAODC_22920: pabC - aminodeoxychorismate lyase, at 1,327,275 to 1,328,084
pabC
MCAODC_22925: fabF - beta-ketoacyl-ACP synthase II, at 1,328,204 to 1,329,445
fabF
Position (kb) 1326
1327
1328 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1326.285 kb on - strand at 1326.325 kb on - strand at 1326.418 kb on - strand, within yceG at 1326.418 kb on - strand, within yceG at 1326.422 kb on - strand, within yceG at 1326.451 kb on - strand, within yceG at 1326.451 kb on - strand, within yceG at 1326.453 kb on - strand, within yceG at 1326.453 kb on - strand, within yceG at 1326.453 kb on - strand, within yceG at 1326.453 kb on - strand, within yceG at 1326.453 kb on - strand, within yceG at 1326.453 kb on - strand, within yceG at 1326.462 kb on - strand, within yceG at 1326.462 kb on - strand, within yceG at 1326.462 kb on - strand, within yceG at 1326.507 kb on - strand, within yceG at 1326.507 kb on - strand, within yceG at 1326.507 kb on - strand, within yceG at 1326.556 kb on - strand, within yceG at 1326.556 kb on - strand, within yceG at 1326.667 kb on - strand, within yceG at 1326.667 kb on - strand, within yceG at 1326.770 kb on + strand, within yceG at 1326.770 kb on + strand, within yceG at 1326.770 kb on + strand, within yceG at 1326.770 kb on + strand, within yceG at 1326.770 kb on + strand, within yceG at 1326.771 kb on - strand, within yceG at 1326.771 kb on - strand, within yceG at 1326.771 kb on - strand, within yceG at 1326.848 kb on + strand, within yceG at 1326.849 kb on - strand, within yceG at 1326.849 kb on - strand, within yceG at 1326.879 kb on - strand, within yceG at 1326.879 kb on - strand, within yceG at 1327.005 kb on + strand, within yceG at 1327.312 kb on + strand at 1327.312 kb on + strand at 1327.313 kb on - strand at 1327.313 kb on - strand at 1327.313 kb on - strand at 1327.314 kb on + strand at 1327.314 kb on + strand at 1327.379 kb on + strand, within pabC at 1327.484 kb on + strand, within pabC at 1327.527 kb on + strand, within pabC at 1327.527 kb on + strand, within pabC at 1327.528 kb on - strand, within pabC at 1327.528 kb on - strand, within pabC at 1327.528 kb on - strand, within pabC at 1327.528 kb on - strand, within pabC at 1327.659 kb on - strand, within pabC at 1327.668 kb on - strand, within pabC at 1327.947 kb on + strand, within pabC at 1328.063 kb on + strand at 1328.064 kb on - strand at 1328.064 kb on - strand at 1328.077 kb on - strand at 1328.077 kb on - strand at 1328.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T2 remove 1,326,285 - -2.0 1,326,325 - +0.1 1,326,418 - yceG MCAODC_22915 0.16 -0.7 1,326,418 - yceG MCAODC_22915 0.16 +0.7 1,326,422 - yceG MCAODC_22915 0.17 +1.9 1,326,451 - yceG MCAODC_22915 0.20 +0.2 1,326,451 - yceG MCAODC_22915 0.20 +0.7 1,326,453 - yceG MCAODC_22915 0.20 -2.2 1,326,453 - yceG MCAODC_22915 0.20 +0.0 1,326,453 - yceG MCAODC_22915 0.20 +1.9 1,326,453 - yceG MCAODC_22915 0.20 -1.5 1,326,453 - yceG MCAODC_22915 0.20 -1.4 1,326,453 - yceG MCAODC_22915 0.20 -1.1 1,326,462 - yceG MCAODC_22915 0.21 +0.6 1,326,462 - yceG MCAODC_22915 0.21 +0.5 1,326,462 - yceG MCAODC_22915 0.21 -1.0 1,326,507 - yceG MCAODC_22915 0.25 +0.3 1,326,507 - yceG MCAODC_22915 0.25 -1.4 1,326,507 - yceG MCAODC_22915 0.25 -2.1 1,326,556 - yceG MCAODC_22915 0.30 -1.1 1,326,556 - yceG MCAODC_22915 0.30 -0.7 1,326,667 - yceG MCAODC_22915 0.41 -0.8 1,326,667 - yceG MCAODC_22915 0.41 -1.1 1,326,770 + yceG MCAODC_22915 0.51 -1.3 1,326,770 + yceG MCAODC_22915 0.51 +2.9 1,326,770 + yceG MCAODC_22915 0.51 -1.2 1,326,770 + yceG MCAODC_22915 0.51 -0.9 1,326,770 + yceG MCAODC_22915 0.51 -1.4 1,326,771 - yceG MCAODC_22915 0.51 -1.8 1,326,771 - yceG MCAODC_22915 0.51 -1.5 1,326,771 - yceG MCAODC_22915 0.51 +1.4 1,326,848 + yceG MCAODC_22915 0.58 -2.1 1,326,849 - yceG MCAODC_22915 0.59 +0.1 1,326,849 - yceG MCAODC_22915 0.59 -2.1 1,326,879 - yceG MCAODC_22915 0.61 -0.7 1,326,879 - yceG MCAODC_22915 0.61 -2.2 1,327,005 + yceG MCAODC_22915 0.74 +1.1 1,327,312 + +1.1 1,327,312 + +0.9 1,327,313 - +1.1 1,327,313 - -0.3 1,327,313 - +2.5 1,327,314 + +1.9 1,327,314 + +1.1 1,327,379 + pabC MCAODC_22920 0.13 -2.0 1,327,484 + pabC MCAODC_22920 0.26 +1.7 1,327,527 + pabC MCAODC_22920 0.31 -1.7 1,327,527 + pabC MCAODC_22920 0.31 +0.3 1,327,528 - pabC MCAODC_22920 0.31 +1.0 1,327,528 - pabC MCAODC_22920 0.31 -0.2 1,327,528 - pabC MCAODC_22920 0.31 -2.2 1,327,528 - pabC MCAODC_22920 0.31 +1.1 1,327,659 - pabC MCAODC_22920 0.47 +1.2 1,327,668 - pabC MCAODC_22920 0.49 -0.8 1,327,947 + pabC MCAODC_22920 0.83 -0.1 1,328,063 + -0.6 1,328,064 - +0.0 1,328,064 - -1.1 1,328,077 - -0.1 1,328,077 - -0.7 1,328,206 - +0.4
Or see this region's nucleotide sequence