Experiment: T2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oRF81 and oRF80 overlap by 1 nucleotides oRF80 and MCAODC_20975 are separated by 63 nucleotides MCAODC_20975 and MCAODC_20980 overlap by 4 nucleotides MCAODC_20980 and MCAODC_20985 are separated by 10 nucleotides MCAODC_20985 and MCAODC_20990 are separated by 121 nucleotides MCAODC_20990 and int are separated by 115 nucleotides
MCAODC_20965: oRF81 - Uncharacterized protein in GpA 5'region, at 999,288 to 999,587
oRF81
MCAODC_20970: oRF80 - Uncharacterized protein in GpA 5'region, at 999,587 to 999,811
oRF80
MCAODC_20975: MCAODC_20975 - Replication gene B protein, at 999,875 to 1,000,375
_20975
MCAODC_20980: MCAODC_20980 - Phage protein B, at 1,000,372 to 1,000,542
_20980
MCAODC_20985: MCAODC_20985 - Regulatory phage protein cox, at 1,000,553 to 1,000,828
_20985
MCAODC_20990: MCAODC_20990 - transcriptional regulator, at 1,000,950 to 1,001,249
_20990
MCAODC_20995: int - Integrase, at 1,001,365 to 1,002,378
int
Position (kb)
1000
1001 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 999.404 kb on + strand, within oRF81 at 999.564 kb on + strand at 1000.217 kb on + strand, within MCAODC_20975 at 1000.217 kb on + strand, within MCAODC_20975 at 1000.269 kb on + strand, within MCAODC_20975 at 1000.270 kb on - strand, within MCAODC_20975 at 1000.273 kb on - strand, within MCAODC_20975 at 1000.273 kb on - strand, within MCAODC_20975 at 1000.282 kb on + strand, within MCAODC_20975 at 1000.283 kb on - strand, within MCAODC_20975 at 1000.330 kb on + strand at 1000.417 kb on + strand, within MCAODC_20980 at 1000.514 kb on - strand, within MCAODC_20980 at 1000.524 kb on + strand, within MCAODC_20980 at 1000.524 kb on + strand, within MCAODC_20980 at 1000.543 kb on - strand at 1000.575 kb on - strand at 1000.575 kb on - strand at 1000.656 kb on + strand, within MCAODC_20985 at 1000.656 kb on + strand, within MCAODC_20985 at 1000.659 kb on - strand, within MCAODC_20985 at 1000.745 kb on + strand, within MCAODC_20985 at 1000.811 kb on + strand at 1000.811 kb on + strand at 1000.812 kb on - strand at 1000.846 kb on + strand at 1000.855 kb on + strand at 1000.876 kb on + strand at 1001.266 kb on + strand at 1001.267 kb on - strand at 1001.283 kb on + strand at 1001.284 kb on - strand at 1001.286 kb on + strand at 1001.328 kb on + strand at 1001.329 kb on - strand at 1001.329 kb on - strand at 1001.395 kb on + strand at 1001.442 kb on + strand at 1001.454 kb on + strand at 1001.494 kb on + strand, within int at 1001.494 kb on + strand, within int at 1001.494 kb on + strand, within int at 1001.494 kb on + strand, within int at 1001.495 kb on - strand, within int
Per-strain Table
Position Strand Gene LocusTag Fraction T2 remove 999,404 + oRF81 MCAODC_20965 0.39 -0.1 999,564 + +0.1 1,000,217 + MCAODC_20975 0.68 +1.0 1,000,217 + MCAODC_20975 0.68 +1.1 1,000,269 + MCAODC_20975 0.79 -3.5 1,000,270 - MCAODC_20975 0.79 +0.8 1,000,273 - MCAODC_20975 0.79 +0.2 1,000,273 - MCAODC_20975 0.79 +0.9 1,000,282 + MCAODC_20975 0.81 -1.2 1,000,283 - MCAODC_20975 0.81 -0.5 1,000,330 + +0.9 1,000,417 + MCAODC_20980 0.26 +0.3 1,000,514 - MCAODC_20980 0.83 +2.5 1,000,524 + MCAODC_20980 0.89 +0.5 1,000,524 + MCAODC_20980 0.89 -0.7 1,000,543 - +0.8 1,000,575 - +1.9 1,000,575 - +1.3 1,000,656 + MCAODC_20985 0.37 +1.2 1,000,656 + MCAODC_20985 0.37 -2.1 1,000,659 - MCAODC_20985 0.38 +0.1 1,000,745 + MCAODC_20985 0.70 -0.3 1,000,811 + -1.8 1,000,811 + +0.5 1,000,812 - +0.2 1,000,846 + +1.0 1,000,855 + +1.1 1,000,876 + -2.3 1,001,266 + -0.5 1,001,267 - +0.6 1,001,283 + -0.9 1,001,284 - +1.0 1,001,286 + -1.3 1,001,328 + +1.5 1,001,329 - +2.4 1,001,329 - +0.4 1,001,395 + +0.6 1,001,442 + -0.4 1,001,454 + +0.2 1,001,494 + int MCAODC_20995 0.13 +1.3 1,001,494 + int MCAODC_20995 0.13 +0.8 1,001,494 + int MCAODC_20995 0.13 -0.2 1,001,494 + int MCAODC_20995 0.13 +1.0 1,001,495 - int MCAODC_20995 0.13 -0.2
Or see this region's nucleotide sequence