Experiment: T2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_17145 and MCAODC_17150 overlap by 4 nucleotides MCAODC_17150 and MCAODC_17155 are separated by 347 nucleotides MCAODC_17155 and MCAODC_17160 overlap by 8 nucleotides MCAODC_17160 and MCAODC_17165 overlap by 4 nucleotides MCAODC_17165 and MCAODC_17170 are separated by 32 nucleotides
MCAODC_17145: MCAODC_17145 - Bacteriophage protein, at 325,289 to 325,372
_17145
MCAODC_17150: MCAODC_17150 - DUF551 domain-containing protein, at 325,369 to 325,926
_17150
MCAODC_17155: MCAODC_17155 - Prophage protein, at 326,274 to 326,585
_17155
MCAODC_17160: MCAODC_17160 - hypothetical protein, at 326,578 to 326,805
_17160
MCAODC_17165: MCAODC_17165 - DNA-binding protein, at 326,802 to 327,083
_17165
MCAODC_17170: MCAODC_17170 - DUF1627 domain-containing protein, at 327,116 to 327,832
_17170
Position (kb)
326
327 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 325.312 kb on - strand, within MCAODC_17145 at 325.450 kb on + strand, within MCAODC_17150 at 325.451 kb on - strand, within MCAODC_17150 at 325.453 kb on - strand, within MCAODC_17150 at 325.496 kb on - strand, within MCAODC_17150 at 325.496 kb on - strand, within MCAODC_17150 at 325.499 kb on - strand, within MCAODC_17150 at 325.533 kb on + strand, within MCAODC_17150 at 325.534 kb on - strand, within MCAODC_17150 at 325.534 kb on - strand, within MCAODC_17150 at 325.595 kb on - strand, within MCAODC_17150 at 325.716 kb on - strand, within MCAODC_17150 at 325.825 kb on - strand, within MCAODC_17150 at 325.887 kb on - strand at 325.915 kb on - strand at 325.994 kb on - strand at 325.996 kb on - strand at 325.997 kb on + strand at 325.997 kb on + strand at 325.997 kb on + strand at 325.998 kb on - strand at 325.998 kb on - strand at 326.043 kb on - strand at 326.072 kb on + strand at 326.073 kb on - strand at 326.073 kb on - strand at 326.246 kb on + strand at 326.388 kb on - strand, within MCAODC_17155 at 326.444 kb on - strand, within MCAODC_17155 at 326.613 kb on - strand, within MCAODC_17160 at 326.642 kb on + strand, within MCAODC_17160 at 326.643 kb on - strand, within MCAODC_17160 at 326.643 kb on - strand, within MCAODC_17160 at 326.643 kb on - strand, within MCAODC_17160 at 326.645 kb on - strand, within MCAODC_17160 at 326.784 kb on - strand at 326.793 kb on - strand at 326.833 kb on + strand, within MCAODC_17165 at 326.845 kb on - strand, within MCAODC_17165 at 326.845 kb on - strand, within MCAODC_17165 at 326.967 kb on + strand, within MCAODC_17165 at 326.967 kb on + strand, within MCAODC_17165 at 326.968 kb on - strand, within MCAODC_17165 at 326.968 kb on - strand, within MCAODC_17165 at 327.048 kb on + strand, within MCAODC_17165 at 327.049 kb on - strand, within MCAODC_17165 at 327.049 kb on - strand, within MCAODC_17165 at 327.078 kb on + strand at 327.078 kb on + strand at 327.078 kb on + strand at 327.146 kb on - strand at 327.554 kb on - strand, within MCAODC_17170
Per-strain Table
Position Strand Gene LocusTag Fraction T2 remove 325,312 - MCAODC_17145 0.27 +0.6 325,450 + MCAODC_17150 0.15 +0.1 325,451 - MCAODC_17150 0.15 -0.2 325,453 - MCAODC_17150 0.15 -0.5 325,496 - MCAODC_17150 0.23 -1.3 325,496 - MCAODC_17150 0.23 +0.7 325,499 - MCAODC_17150 0.23 -0.8 325,533 + MCAODC_17150 0.29 +0.5 325,534 - MCAODC_17150 0.30 +0.4 325,534 - MCAODC_17150 0.30 -0.4 325,595 - MCAODC_17150 0.41 -2.1 325,716 - MCAODC_17150 0.62 +1.7 325,825 - MCAODC_17150 0.82 +0.8 325,887 - -0.0 325,915 - +1.5 325,994 - +0.1 325,996 - -1.2 325,997 + -2.2 325,997 + +0.1 325,997 + -0.4 325,998 - +1.4 325,998 - +1.2 326,043 - +1.1 326,072 + -0.5 326,073 - +1.2 326,073 - -1.9 326,246 + -0.6 326,388 - MCAODC_17155 0.37 -0.2 326,444 - MCAODC_17155 0.54 -2.3 326,613 - MCAODC_17160 0.15 -0.4 326,642 + MCAODC_17160 0.28 -0.5 326,643 - MCAODC_17160 0.29 +2.2 326,643 - MCAODC_17160 0.29 +1.5 326,643 - MCAODC_17160 0.29 -0.3 326,645 - MCAODC_17160 0.29 -0.3 326,784 - -0.6 326,793 - +1.0 326,833 + MCAODC_17165 0.11 +0.1 326,845 - MCAODC_17165 0.15 -0.6 326,845 - MCAODC_17165 0.15 -0.4 326,967 + MCAODC_17165 0.59 +2.8 326,967 + MCAODC_17165 0.59 +0.4 326,968 - MCAODC_17165 0.59 +0.4 326,968 - MCAODC_17165 0.59 +1.4 327,048 + MCAODC_17165 0.87 +1.1 327,049 - MCAODC_17165 0.88 +1.6 327,049 - MCAODC_17165 0.88 +0.1 327,078 + +0.4 327,078 + +0.2 327,078 + +0.4 327,146 - -0.7 327,554 - MCAODC_17170 0.61 +0.2
Or see this region's nucleotide sequence