Experiment: T2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfgH and yfgG are separated by 313 nucleotides yfgG and MCAODC_12315 are separated by 35 nucleotides MCAODC_12315 and pdeF are separated by 181 nucleotides
MCAODC_12305: yfgH - Uncharacterized lipoprotein YfgH, at 2,438,513 to 2,439,028
yfgH
MCAODC_12310: yfgG - Uncharacterized protein YfgG, at 2,439,342 to 2,439,515
yfgG
MCAODC_12315: MCAODC_12315 - hypothetical protein, at 2,439,551 to 2,439,703
_12315
MCAODC_12320: pdeF - cyclic-guanylate-specific phosphodiesterase PdeF, at 2,439,885 to 2,442,128
pdeF
Position (kb)
2439
2440 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2438.563 kb on - strand at 2438.575 kb on + strand, within yfgH at 2438.575 kb on + strand, within yfgH at 2438.597 kb on - strand, within yfgH at 2438.624 kb on - strand, within yfgH at 2438.629 kb on + strand, within yfgH at 2438.629 kb on + strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.631 kb on + strand, within yfgH at 2438.631 kb on + strand, within yfgH at 2438.631 kb on + strand, within yfgH at 2438.632 kb on - strand, within yfgH at 2438.632 kb on - strand, within yfgH at 2438.632 kb on - strand, within yfgH at 2438.650 kb on + strand, within yfgH at 2438.650 kb on + strand, within yfgH at 2438.652 kb on + strand, within yfgH at 2438.652 kb on + strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.676 kb on - strand, within yfgH at 2438.676 kb on - strand, within yfgH at 2438.695 kb on + strand, within yfgH at 2438.696 kb on - strand, within yfgH at 2438.931 kb on + strand, within yfgH at 2439.045 kb on - strand at 2439.430 kb on + strand, within yfgG at 2439.431 kb on - strand, within yfgG at 2439.435 kb on - strand, within yfgG at 2439.472 kb on - strand, within yfgG at 2439.515 kb on + strand at 2439.551 kb on - strand at 2439.575 kb on + strand, within MCAODC_12315 at 2439.576 kb on - strand, within MCAODC_12315 at 2439.687 kb on + strand, within MCAODC_12315 at 2439.688 kb on - strand at 2439.702 kb on - strand at 2439.718 kb on + strand at 2439.748 kb on + strand at 2439.750 kb on + strand at 2439.751 kb on - strand at 2439.796 kb on - strand at 2439.797 kb on + strand at 2439.798 kb on - strand at 2439.845 kb on + strand at 2439.903 kb on + strand at 2439.956 kb on - strand at 2440.113 kb on + strand, within pdeF at 2440.160 kb on + strand, within pdeF at 2440.169 kb on + strand, within pdeF at 2440.169 kb on + strand, within pdeF at 2440.203 kb on - strand, within pdeF at 2440.203 kb on - strand, within pdeF at 2440.256 kb on + strand, within pdeF at 2440.256 kb on + strand, within pdeF at 2440.256 kb on + strand, within pdeF at 2440.257 kb on - strand, within pdeF at 2440.322 kb on - strand, within pdeF at 2440.381 kb on + strand, within pdeF at 2440.382 kb on - strand, within pdeF at 2440.434 kb on + strand, within pdeF at 2440.435 kb on - strand, within pdeF at 2440.475 kb on + strand, within pdeF at 2440.492 kb on + strand, within pdeF at 2440.552 kb on + strand, within pdeF at 2440.562 kb on - strand, within pdeF at 2440.562 kb on - strand, within pdeF at 2440.573 kb on + strand, within pdeF at 2440.617 kb on + strand, within pdeF at 2440.618 kb on - strand, within pdeF at 2440.620 kb on - strand, within pdeF at 2440.671 kb on + strand, within pdeF at 2440.671 kb on + strand, within pdeF at 2440.672 kb on - strand, within pdeF at 2440.672 kb on - strand, within pdeF at 2440.673 kb on + strand, within pdeF at 2440.673 kb on + strand, within pdeF at 2440.673 kb on + strand, within pdeF at 2440.674 kb on - strand, within pdeF at 2440.674 kb on - strand, within pdeF at 2440.674 kb on - strand, within pdeF at 2440.674 kb on - strand, within pdeF at 2440.674 kb on - strand, within pdeF
Per-strain Table
Position Strand Gene LocusTag Fraction T2 remove 2,438,563 - +0.6 2,438,575 + yfgH MCAODC_12305 0.12 -0.6 2,438,575 + yfgH MCAODC_12305 0.12 -0.4 2,438,597 - yfgH MCAODC_12305 0.16 -2.9 2,438,624 - yfgH MCAODC_12305 0.22 -0.1 2,438,629 + yfgH MCAODC_12305 0.22 -2.1 2,438,629 + yfgH MCAODC_12305 0.22 -2.5 2,438,630 - yfgH MCAODC_12305 0.23 -0.3 2,438,630 - yfgH MCAODC_12305 0.23 -0.9 2,438,630 - yfgH MCAODC_12305 0.23 -0.3 2,438,630 - yfgH MCAODC_12305 0.23 +0.0 2,438,630 - yfgH MCAODC_12305 0.23 +0.3 2,438,631 + yfgH MCAODC_12305 0.23 -0.0 2,438,631 + yfgH MCAODC_12305 0.23 +3.0 2,438,631 + yfgH MCAODC_12305 0.23 -0.6 2,438,632 - yfgH MCAODC_12305 0.23 -0.3 2,438,632 - yfgH MCAODC_12305 0.23 +0.2 2,438,632 - yfgH MCAODC_12305 0.23 +1.3 2,438,650 + yfgH MCAODC_12305 0.27 -1.3 2,438,650 + yfgH MCAODC_12305 0.27 +3.0 2,438,652 + yfgH MCAODC_12305 0.27 +2.4 2,438,652 + yfgH MCAODC_12305 0.27 -0.0 2,438,653 - yfgH MCAODC_12305 0.27 -2.2 2,438,653 - yfgH MCAODC_12305 0.27 -0.6 2,438,653 - yfgH MCAODC_12305 0.27 +1.3 2,438,653 - yfgH MCAODC_12305 0.27 -0.4 2,438,653 - yfgH MCAODC_12305 0.27 +0.4 2,438,676 - yfgH MCAODC_12305 0.32 +1.3 2,438,676 - yfgH MCAODC_12305 0.32 -0.6 2,438,695 + yfgH MCAODC_12305 0.35 -0.0 2,438,696 - yfgH MCAODC_12305 0.35 +0.3 2,438,931 + yfgH MCAODC_12305 0.81 -0.9 2,439,045 - -0.5 2,439,430 + yfgG MCAODC_12310 0.51 +0.9 2,439,431 - yfgG MCAODC_12310 0.51 +1.6 2,439,435 - yfgG MCAODC_12310 0.53 +0.4 2,439,472 - yfgG MCAODC_12310 0.75 -1.1 2,439,515 + +1.2 2,439,551 - +1.1 2,439,575 + MCAODC_12315 0.16 -0.5 2,439,576 - MCAODC_12315 0.16 -0.7 2,439,687 + MCAODC_12315 0.89 -2.8 2,439,688 - +0.9 2,439,702 - +0.0 2,439,718 + -0.6 2,439,748 + -0.8 2,439,750 + -2.3 2,439,751 - -1.0 2,439,796 - +0.9 2,439,797 + +1.5 2,439,798 - -0.9 2,439,845 + +0.6 2,439,903 + +0.1 2,439,956 - +1.5 2,440,113 + pdeF MCAODC_12320 0.10 +0.5 2,440,160 + pdeF MCAODC_12320 0.12 +2.3 2,440,169 + pdeF MCAODC_12320 0.13 +1.4 2,440,169 + pdeF MCAODC_12320 0.13 +0.9 2,440,203 - pdeF MCAODC_12320 0.14 -0.3 2,440,203 - pdeF MCAODC_12320 0.14 -1.2 2,440,256 + pdeF MCAODC_12320 0.17 +2.0 2,440,256 + pdeF MCAODC_12320 0.17 -1.5 2,440,256 + pdeF MCAODC_12320 0.17 +0.7 2,440,257 - pdeF MCAODC_12320 0.17 -0.1 2,440,322 - pdeF MCAODC_12320 0.19 +0.3 2,440,381 + pdeF MCAODC_12320 0.22 +0.1 2,440,382 - pdeF MCAODC_12320 0.22 +0.2 2,440,434 + pdeF MCAODC_12320 0.24 -0.0 2,440,435 - pdeF MCAODC_12320 0.25 -0.9 2,440,475 + pdeF MCAODC_12320 0.26 -0.1 2,440,492 + pdeF MCAODC_12320 0.27 -2.4 2,440,552 + pdeF MCAODC_12320 0.30 +2.4 2,440,562 - pdeF MCAODC_12320 0.30 +1.1 2,440,562 - pdeF MCAODC_12320 0.30 -0.6 2,440,573 + pdeF MCAODC_12320 0.31 +0.6 2,440,617 + pdeF MCAODC_12320 0.33 +0.3 2,440,618 - pdeF MCAODC_12320 0.33 +0.8 2,440,620 - pdeF MCAODC_12320 0.33 -1.4 2,440,671 + pdeF MCAODC_12320 0.35 -1.3 2,440,671 + pdeF MCAODC_12320 0.35 -1.5 2,440,672 - pdeF MCAODC_12320 0.35 -0.3 2,440,672 - pdeF MCAODC_12320 0.35 -0.2 2,440,673 + pdeF MCAODC_12320 0.35 -0.1 2,440,673 + pdeF MCAODC_12320 0.35 +0.3 2,440,673 + pdeF MCAODC_12320 0.35 -0.8 2,440,674 - pdeF MCAODC_12320 0.35 -0.0 2,440,674 - pdeF MCAODC_12320 0.35 -0.4 2,440,674 - pdeF MCAODC_12320 0.35 +1.7 2,440,674 - pdeF MCAODC_12320 0.35 -0.2 2,440,674 - pdeF MCAODC_12320 0.35 -1.9
Or see this region's nucleotide sequence