Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt opgE and MCAODC_25405 are separated by 48 nucleotides MCAODC_25405 and yliM are separated by 52 nucleotides yliM and rhtA are separated by 234 nucleotides
MCAODC_25400: opgE - phosphoethanolamine transferase, at 1,803,802 to 1,805,385
opgE
MCAODC_25405: MCAODC_25405 - Outer membrane protein X, at 1,805,434 to 1,805,949
_25405
MCAODC_25410: yliM - Protein YliM, at 1,806,002 to 1,806,067
yliM
MCAODC_25415: rhtA - threonine/homoserine exporter RhtA, at 1,806,302 to 1,807,189
rhtA
Position (kb)
1805
1806 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1804.456 kb on + strand, within opgE at 1804.456 kb on + strand, within opgE at 1804.456 kb on + strand, within opgE at 1804.456 kb on + strand, within opgE at 1804.457 kb on - strand, within opgE at 1804.723 kb on + strand, within opgE at 1804.735 kb on - strand, within opgE at 1804.768 kb on + strand, within opgE at 1804.768 kb on + strand, within opgE at 1804.769 kb on - strand, within opgE at 1804.769 kb on - strand, within opgE at 1804.933 kb on + strand, within opgE at 1804.969 kb on + strand, within opgE at 1804.975 kb on + strand, within opgE at 1804.975 kb on + strand, within opgE at 1804.975 kb on + strand, within opgE at 1804.976 kb on - strand, within opgE at 1804.977 kb on + strand, within opgE at 1804.977 kb on + strand, within opgE at 1804.977 kb on + strand, within opgE at 1804.980 kb on + strand, within opgE at 1804.980 kb on + strand, within opgE at 1804.981 kb on - strand, within opgE at 1805.000 kb on + strand, within opgE at 1805.000 kb on + strand, within opgE at 1805.015 kb on - strand, within opgE at 1805.107 kb on + strand, within opgE at 1805.107 kb on + strand, within opgE at 1805.107 kb on + strand, within opgE at 1805.124 kb on + strand, within opgE at 1805.128 kb on + strand, within opgE at 1805.155 kb on - strand, within opgE at 1805.222 kb on + strand, within opgE at 1805.251 kb on - strand at 1805.265 kb on + strand at 1805.275 kb on + strand at 1805.283 kb on + strand at 1805.284 kb on - strand at 1805.311 kb on + strand at 1805.311 kb on + strand at 1805.311 kb on + strand at 1805.311 kb on + strand at 1805.312 kb on - strand at 1805.312 kb on - strand at 1805.312 kb on - strand at 1805.312 kb on - strand at 1805.312 kb on - strand at 1805.312 kb on - strand at 1805.312 kb on - strand at 1805.344 kb on + strand at 1805.470 kb on + strand at 1805.471 kb on - strand at 1805.471 kb on - strand at 1805.556 kb on - strand, within MCAODC_25405 at 1805.556 kb on - strand, within MCAODC_25405 at 1805.556 kb on - strand, within MCAODC_25405 at 1805.589 kb on - strand, within MCAODC_25405 at 1805.616 kb on + strand, within MCAODC_25405 at 1805.636 kb on - strand, within MCAODC_25405 at 1805.643 kb on - strand, within MCAODC_25405 at 1805.662 kb on - strand, within MCAODC_25405 at 1805.694 kb on - strand, within MCAODC_25405 at 1805.697 kb on - strand, within MCAODC_25405 at 1805.697 kb on - strand, within MCAODC_25405 at 1805.748 kb on - strand, within MCAODC_25405 at 1805.748 kb on - strand, within MCAODC_25405 at 1805.799 kb on - strand, within MCAODC_25405 at 1805.799 kb on - strand, within MCAODC_25405 at 1805.872 kb on - strand, within MCAODC_25405 at 1805.885 kb on - strand, within MCAODC_25405 at 1805.893 kb on - strand, within MCAODC_25405 at 1805.973 kb on + strand at 1805.973 kb on + strand at 1805.974 kb on - strand at 1805.994 kb on - strand at 1806.115 kb on - strand at 1806.119 kb on - strand at 1806.121 kb on + strand at 1806.122 kb on - strand at 1806.153 kb on - strand at 1806.170 kb on + strand at 1806.171 kb on - strand at 1806.171 kb on - strand at 1806.199 kb on - strand at 1806.248 kb on + strand at 1806.251 kb on - strand at 1806.258 kb on - strand at 1806.271 kb on + strand at 1806.275 kb on + strand at 1806.275 kb on + strand at 1806.275 kb on + strand at 1806.275 kb on + strand at 1806.276 kb on - strand at 1806.285 kb on + strand at 1806.285 kb on + strand at 1806.286 kb on - strand at 1806.315 kb on + strand at 1806.315 kb on + strand at 1806.320 kb on - strand at 1806.343 kb on - strand at 1806.343 kb on - strand at 1806.348 kb on + strand at 1806.348 kb on + strand at 1806.348 kb on + strand at 1806.370 kb on + strand at 1806.370 kb on + strand at 1806.370 kb on + strand at 1806.371 kb on - strand at 1806.391 kb on - strand, within rhtA at 1806.394 kb on + strand, within rhtA at 1806.451 kb on - strand, within rhtA at 1806.451 kb on - strand, within rhtA at 1806.530 kb on + strand, within rhtA at 1806.530 kb on + strand, within rhtA at 1806.531 kb on - strand, within rhtA at 1806.567 kb on - strand, within rhtA at 1806.574 kb on + strand, within rhtA at 1806.575 kb on - strand, within rhtA at 1806.586 kb on - strand, within rhtA at 1806.596 kb on - strand, within rhtA at 1806.784 kb on + strand, within rhtA at 1806.864 kb on + strand, within rhtA
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 1,804,456 + opgE MCAODC_25400 0.41 +2.3 1,804,456 + opgE MCAODC_25400 0.41 +3.2 1,804,456 + opgE MCAODC_25400 0.41 -1.3 1,804,456 + opgE MCAODC_25400 0.41 -1.6 1,804,457 - opgE MCAODC_25400 0.41 +0.6 1,804,723 + opgE MCAODC_25400 0.58 +0.4 1,804,735 - opgE MCAODC_25400 0.59 +1.9 1,804,768 + opgE MCAODC_25400 0.61 -0.1 1,804,768 + opgE MCAODC_25400 0.61 -0.7 1,804,769 - opgE MCAODC_25400 0.61 -1.5 1,804,769 - opgE MCAODC_25400 0.61 +1.8 1,804,933 + opgE MCAODC_25400 0.71 +0.5 1,804,969 + opgE MCAODC_25400 0.74 +0.6 1,804,975 + opgE MCAODC_25400 0.74 -3.1 1,804,975 + opgE MCAODC_25400 0.74 +0.1 1,804,975 + opgE MCAODC_25400 0.74 -1.2 1,804,976 - opgE MCAODC_25400 0.74 -0.5 1,804,977 + opgE MCAODC_25400 0.74 -0.5 1,804,977 + opgE MCAODC_25400 0.74 -0.9 1,804,977 + opgE MCAODC_25400 0.74 +1.8 1,804,980 + opgE MCAODC_25400 0.74 -0.4 1,804,980 + opgE MCAODC_25400 0.74 -0.2 1,804,981 - opgE MCAODC_25400 0.74 -0.2 1,805,000 + opgE MCAODC_25400 0.76 +0.1 1,805,000 + opgE MCAODC_25400 0.76 -0.3 1,805,015 - opgE MCAODC_25400 0.77 +0.3 1,805,107 + opgE MCAODC_25400 0.82 -0.9 1,805,107 + opgE MCAODC_25400 0.82 +0.7 1,805,107 + opgE MCAODC_25400 0.82 -3.2 1,805,124 + opgE MCAODC_25400 0.83 +2.0 1,805,128 + opgE MCAODC_25400 0.84 +0.4 1,805,155 - opgE MCAODC_25400 0.85 +2.0 1,805,222 + opgE MCAODC_25400 0.90 +0.1 1,805,251 - +1.5 1,805,265 + +1.1 1,805,275 + -0.6 1,805,283 + -0.5 1,805,284 - +0.4 1,805,311 + +0.1 1,805,311 + -0.4 1,805,311 + +1.8 1,805,311 + +0.5 1,805,312 - +1.6 1,805,312 - -0.0 1,805,312 - -0.6 1,805,312 - -0.1 1,805,312 - -0.9 1,805,312 - -0.4 1,805,312 - -1.6 1,805,344 + +0.1 1,805,470 + -3.3 1,805,471 - -0.8 1,805,471 - -3.5 1,805,556 - MCAODC_25405 0.24 -1.2 1,805,556 - MCAODC_25405 0.24 -1.1 1,805,556 - MCAODC_25405 0.24 -0.2 1,805,589 - MCAODC_25405 0.30 -1.1 1,805,616 + MCAODC_25405 0.35 -2.7 1,805,636 - MCAODC_25405 0.39 -1.8 1,805,643 - MCAODC_25405 0.41 -2.5 1,805,662 - MCAODC_25405 0.44 -1.1 1,805,694 - MCAODC_25405 0.50 -1.7 1,805,697 - MCAODC_25405 0.51 -0.3 1,805,697 - MCAODC_25405 0.51 -1.5 1,805,748 - MCAODC_25405 0.61 -3.6 1,805,748 - MCAODC_25405 0.61 -1.5 1,805,799 - MCAODC_25405 0.71 -1.9 1,805,799 - MCAODC_25405 0.71 -1.3 1,805,872 - MCAODC_25405 0.85 -0.5 1,805,885 - MCAODC_25405 0.87 -0.5 1,805,893 - MCAODC_25405 0.89 -4.0 1,805,973 + -2.0 1,805,973 + -1.3 1,805,974 - -1.0 1,805,994 - -1.4 1,806,115 - -1.1 1,806,119 - +0.8 1,806,121 + -1.4 1,806,122 - +0.3 1,806,153 - -1.1 1,806,170 + -0.6 1,806,171 - +0.2 1,806,171 - -0.7 1,806,199 - -0.3 1,806,248 + +1.0 1,806,251 - +0.5 1,806,258 - -0.5 1,806,271 + +0.3 1,806,275 + -1.3 1,806,275 + +1.2 1,806,275 + +0.6 1,806,275 + +1.0 1,806,276 - +1.4 1,806,285 + -1.5 1,806,285 + -0.9 1,806,286 - +1.1 1,806,315 + +0.1 1,806,315 + -0.2 1,806,320 - -0.2 1,806,343 - +1.3 1,806,343 - +0.9 1,806,348 + +0.4 1,806,348 + -0.4 1,806,348 + +0.3 1,806,370 + -0.7 1,806,370 + +0.6 1,806,370 + -0.1 1,806,371 - +0.4 1,806,391 - rhtA MCAODC_25415 0.10 +1.0 1,806,394 + rhtA MCAODC_25415 0.10 +0.1 1,806,451 - rhtA MCAODC_25415 0.17 -0.2 1,806,451 - rhtA MCAODC_25415 0.17 +1.8 1,806,530 + rhtA MCAODC_25415 0.26 +0.3 1,806,530 + rhtA MCAODC_25415 0.26 +0.4 1,806,531 - rhtA MCAODC_25415 0.26 -0.4 1,806,567 - rhtA MCAODC_25415 0.30 -1.4 1,806,574 + rhtA MCAODC_25415 0.31 -1.6 1,806,575 - rhtA MCAODC_25415 0.31 +3.3 1,806,586 - rhtA MCAODC_25415 0.32 -0.3 1,806,596 - rhtA MCAODC_25415 0.33 +0.2 1,806,784 + rhtA MCAODC_25415 0.54 +0.3 1,806,864 + rhtA MCAODC_25415 0.63 +0.2
Or see this region's nucleotide sequence