Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22075 and PS417_22080 are separated by 57 nucleotides PS417_22080 and PS417_22085 are separated by 55 nucleotides PS417_22085 and PS417_22090 are separated by 205 nucleotides PS417_22090 and PS417_22095 are separated by 12 nucleotides
PS417_22075: PS417_22075 - alpha/beta hydrolase, at 4,784,306 to 4,785,250
_22075
PS417_22080: PS417_22080 - 3-hydroxybutyryl-CoA dehydratase, at 4,785,308 to 4,785,778
_22080
PS417_22085: PS417_22085 - aldehyde dehydrogenase, at 4,785,834 to 4,786,301
_22085
PS417_22090: PS417_22090 - hypothetical protein, at 4,786,507 to 4,786,743
_22090
PS417_22095: PS417_22095 - class V aminotransferase, at 4,786,756 to 4,787,931
_22095
Position (kb)
4785
4786
4787 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4784.906 kb on + strand, within PS417_22075 at 4784.906 kb on + strand, within PS417_22075 at 4784.907 kb on - strand, within PS417_22075 at 4785.195 kb on + strand at 4785.196 kb on - strand at 4785.857 kb on + strand at 4785.892 kb on - strand, within PS417_22085 at 4785.901 kb on + strand, within PS417_22085 at 4785.901 kb on + strand, within PS417_22085 at 4786.424 kb on - strand at 4786.562 kb on - strand, within PS417_22090 at 4786.562 kb on - strand, within PS417_22090 at 4786.755 kb on + strand at 4786.755 kb on + strand at 4786.756 kb on - strand at 4786.756 kb on - strand at 4786.902 kb on + strand, within PS417_22095 at 4786.928 kb on + strand, within PS417_22095 at 4786.928 kb on + strand, within PS417_22095 at 4787.059 kb on + strand, within PS417_22095 at 4787.060 kb on - strand, within PS417_22095 at 4787.060 kb on - strand, within PS417_22095 at 4787.088 kb on - strand, within PS417_22095 at 4787.215 kb on + strand, within PS417_22095
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 4,784,906 + PS417_22075 0.63 -0.8 4,784,906 + PS417_22075 0.63 -0.7 4,784,907 - PS417_22075 0.64 -1.7 4,785,195 + -0.1 4,785,196 - +1.3 4,785,857 + +0.8 4,785,892 - PS417_22085 0.12 +0.2 4,785,901 + PS417_22085 0.14 -1.7 4,785,901 + PS417_22085 0.14 -0.7 4,786,424 - +0.3 4,786,562 - PS417_22090 0.23 +3.0 4,786,562 - PS417_22090 0.23 -0.3 4,786,755 + +1.3 4,786,755 + +0.3 4,786,756 - -0.4 4,786,756 - +0.9 4,786,902 + PS417_22095 0.12 +0.2 4,786,928 + PS417_22095 0.15 +0.5 4,786,928 + PS417_22095 0.15 -0.5 4,787,059 + PS417_22095 0.26 -1.8 4,787,060 - PS417_22095 0.26 +2.5 4,787,060 - PS417_22095 0.26 -0.5 4,787,088 - PS417_22095 0.28 -2.0 4,787,215 + PS417_22095 0.39 +0.5
Or see this region's nucleotide sequence