Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19345 and PS417_19350 are separated by 78 nucleotides PS417_19350 and PS417_19355 are separated by 101 nucleotides PS417_19355 and PS417_19360 are separated by 22 nucleotides PS417_19360 and PS417_19365 overlap by 4 nucleotides
PS417_19345: PS417_19345 - DNA mismatch repair protein MutS, at 4,194,341 to 4,194,898
_19345
PS417_19350: PS417_19350 - GTP cyclohydrolase, at 4,194,977 to 4,195,522
_19350
PS417_19355: PS417_19355 - glutaredoxin, at 4,195,624 to 4,195,995
_19355
PS417_19360: PS417_19360 - hypothetical protein, at 4,196,018 to 4,196,338
_19360
PS417_19365: PS417_19365 - ABC transporter substrate-binding protein, at 4,196,335 to 4,197,660
_19365
Position (kb) 4195
4196 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4194.854 kb on + strand at 4194.854 kb on + strand at 4194.854 kb on + strand at 4194.854 kb on + strand at 4194.854 kb on + strand at 4194.855 kb on - strand at 4194.855 kb on - strand at 4194.855 kb on - strand at 4194.855 kb on - strand at 4194.995 kb on + strand at 4194.996 kb on - strand at 4195.185 kb on - strand, within PS417_19350 at 4195.233 kb on + strand, within PS417_19350 at 4195.238 kb on + strand, within PS417_19350 at 4195.238 kb on + strand, within PS417_19350 at 4195.239 kb on - strand, within PS417_19350 at 4195.239 kb on - strand, within PS417_19350 at 4195.239 kb on - strand, within PS417_19350 at 4195.295 kb on + strand, within PS417_19350 at 4195.520 kb on + strand at 4195.584 kb on + strand at 4195.585 kb on - strand at 4195.912 kb on - strand, within PS417_19355 at 4195.942 kb on + strand, within PS417_19355 at 4195.942 kb on + strand at 4195.942 kb on + strand, within PS417_19355 at 4195.942 kb on + strand, within PS417_19355 at 4195.942 kb on + strand, within PS417_19355 at 4195.943 kb on - strand, within PS417_19355 at 4195.943 kb on - strand at 4195.943 kb on - strand at 4195.967 kb on - strand at 4196.002 kb on + strand at 4196.002 kb on + strand at 4196.020 kb on - strand at 4196.020 kb on - strand at 4196.250 kb on + strand, within PS417_19360 at 4196.281 kb on + strand, within PS417_19360 at 4196.282 kb on - strand, within PS417_19360 at 4196.405 kb on - strand at 4196.611 kb on + strand, within PS417_19365 at 4196.657 kb on - strand, within PS417_19365 at 4196.784 kb on + strand at 4196.840 kb on + strand, within PS417_19365 at 4196.841 kb on - strand, within PS417_19365 at 4196.865 kb on - strand, within PS417_19365 at 4196.865 kb on - strand, within PS417_19365 at 4196.868 kb on + strand, within PS417_19365 at 4196.868 kb on + strand, within PS417_19365 at 4196.868 kb on + strand, within PS417_19365 at 4196.868 kb on + strand, within PS417_19365 at 4196.869 kb on - strand at 4196.869 kb on - strand, within PS417_19365 at 4196.926 kb on - strand, within PS417_19365
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 4,194,854 + -0.1 4,194,854 + +0.6 4,194,854 + -1.1 4,194,854 + -0.6 4,194,854 + +0.5 4,194,855 - +0.3 4,194,855 - -0.4 4,194,855 - +1.0 4,194,855 - +0.5 4,194,995 + +3.2 4,194,996 - +1.1 4,195,185 - PS417_19350 0.38 +1.2 4,195,233 + PS417_19350 0.47 +3.3 4,195,238 + PS417_19350 0.48 +1.2 4,195,238 + PS417_19350 0.48 +3.5 4,195,239 - PS417_19350 0.48 +1.4 4,195,239 - PS417_19350 0.48 +4.2 4,195,239 - PS417_19350 0.48 +2.2 4,195,295 + PS417_19350 0.58 +2.1 4,195,520 + +0.0 4,195,584 + +0.3 4,195,585 - -0.5 4,195,912 - PS417_19355 0.77 +1.3 4,195,942 + PS417_19355 0.85 +1.1 4,195,942 + +0.1 4,195,942 + PS417_19355 0.85 +0.0 4,195,942 + PS417_19355 0.85 +0.9 4,195,942 + PS417_19355 0.85 +0.4 4,195,943 - PS417_19355 0.86 -0.3 4,195,943 - -0.9 4,195,943 - +0.5 4,195,967 - +0.0 4,196,002 + +0.1 4,196,002 + +0.1 4,196,020 - -2.3 4,196,020 - +1.6 4,196,250 + PS417_19360 0.72 -0.9 4,196,281 + PS417_19360 0.82 +0.3 4,196,282 - PS417_19360 0.82 +0.2 4,196,405 - +1.5 4,196,611 + PS417_19365 0.21 +0.4 4,196,657 - PS417_19365 0.24 -0.4 4,196,784 + -0.9 4,196,840 + PS417_19365 0.38 -0.1 4,196,841 - PS417_19365 0.38 +0.1 4,196,865 - PS417_19365 0.40 -0.4 4,196,865 - PS417_19365 0.40 -2.3 4,196,868 + PS417_19365 0.40 -1.0 4,196,868 + PS417_19365 0.40 +0.1 4,196,868 + PS417_19365 0.40 +0.2 4,196,868 + PS417_19365 0.40 +1.4 4,196,869 - +3.2 4,196,869 - PS417_19365 0.40 +0.3 4,196,926 - PS417_19365 0.45 -0.1
Or see this region's nucleotide sequence