Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01425 and PS417_01430 are separated by 533 nucleotides PS417_01430 and PS417_01435 overlap by 4 nucleotides PS417_01435 and PS417_01440 overlap by 11 nucleotides
PS417_01425: PS417_01425 - AraC family transcriptional regulator, at 315,999 to 316,868
_01425
PS417_01430: PS417_01430 - chromosome segregation protein SMC, at 317,402 to 319,762
_01430
PS417_01435: PS417_01435 - hypothetical protein, at 319,759 to 320,478
_01435
PS417_01440: PS417_01440 - hypothetical protein, at 320,468 to 321,907
_01440
Position (kb)
317
318
319
320 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 316.595 kb on - strand, within PS417_01425 at 316.851 kb on + strand at 316.852 kb on - strand at 316.852 kb on - strand at 316.852 kb on - strand at 316.998 kb on + strand at 316.998 kb on + strand at 316.999 kb on - strand at 316.999 kb on - strand at 317.022 kb on + strand at 317.022 kb on + strand at 317.023 kb on - strand at 317.023 kb on - strand at 317.101 kb on + strand at 317.146 kb on - strand at 317.155 kb on + strand at 317.156 kb on - strand at 317.158 kb on + strand at 317.158 kb on + strand at 317.158 kb on + strand at 317.159 kb on - strand at 317.159 kb on - strand at 317.177 kb on + strand at 317.227 kb on - strand at 317.291 kb on + strand at 317.292 kb on - strand at 317.292 kb on - strand at 317.707 kb on + strand, within PS417_01430 at 318.308 kb on - strand, within PS417_01430 at 318.897 kb on + strand, within PS417_01430 at 318.998 kb on + strand, within PS417_01430 at 319.299 kb on - strand, within PS417_01430 at 319.475 kb on + strand, within PS417_01430 at 319.555 kb on + strand at 319.555 kb on + strand at 319.556 kb on - strand at 319.844 kb on + strand, within PS417_01435
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 316,595 - PS417_01425 0.69 -0.1 316,851 + -0.3 316,852 - -0.2 316,852 - -2.1 316,852 - +0.5 316,998 + -0.6 316,998 + +0.4 316,999 - +1.1 316,999 - +2.0 317,022 + +1.4 317,022 + -0.5 317,023 - -0.5 317,023 - -0.8 317,101 + -2.1 317,146 - -0.8 317,155 + +1.5 317,156 - -0.3 317,158 + -0.2 317,158 + -1.4 317,158 + +1.4 317,159 - -2.6 317,159 - +0.3 317,177 + +0.2 317,227 - -1.0 317,291 + -0.2 317,292 - +0.7 317,292 - -0.8 317,707 + PS417_01430 0.13 +0.4 318,308 - PS417_01430 0.38 +0.6 318,897 + PS417_01430 0.63 +1.5 318,998 + PS417_01430 0.68 -0.7 319,299 - PS417_01430 0.80 +1.0 319,475 + PS417_01430 0.88 +0.6 319,555 + -0.3 319,555 + +0.0 319,556 - +0.4 319,844 + PS417_01435 0.12 -0.1
Or see this region's nucleotide sequence