Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10730 and PS417_10735 are separated by 37 nucleotides PS417_10735 and PS417_10740 are separated by 75 nucleotides PS417_10740 and PS417_10745 are separated by 5 nucleotides PS417_10745 and PS417_10750 are separated by 129 nucleotides
PS417_10730: PS417_10730 - hypothetical protein, at 2,293,199 to 2,294,659
_10730
PS417_10735: PS417_10735 - hypothetical protein, at 2,294,697 to 2,295,224
_10735
PS417_10740: PS417_10740 - hypothetical protein, at 2,295,300 to 2,295,512
_10740
PS417_10745: PS417_10745 - hypothetical protein, at 2,295,518 to 2,296,192
_10745
PS417_10750: PS417_10750 - hypothetical protein, at 2,296,322 to 2,296,627
_10750
Position (kb)
2295
2296 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2294.307 kb on + strand, within PS417_10730 at 2294.307 kb on + strand, within PS417_10730 at 2294.308 kb on - strand, within PS417_10730 at 2294.542 kb on + strand at 2294.542 kb on + strand at 2294.542 kb on + strand at 2294.542 kb on + strand at 2294.542 kb on + strand at 2294.571 kb on - strand at 2294.657 kb on + strand at 2294.658 kb on - strand at 2294.741 kb on + strand at 2294.742 kb on - strand at 2294.742 kb on - strand at 2294.757 kb on - strand, within PS417_10735 at 2294.874 kb on + strand, within PS417_10735 at 2294.874 kb on + strand, within PS417_10735 at 2294.898 kb on - strand, within PS417_10735 at 2294.901 kb on + strand, within PS417_10735 at 2294.902 kb on - strand, within PS417_10735 at 2295.081 kb on - strand, within PS417_10735 at 2295.085 kb on - strand, within PS417_10735 at 2295.096 kb on - strand, within PS417_10735 at 2295.223 kb on - strand at 2295.293 kb on + strand at 2295.294 kb on - strand at 2295.357 kb on + strand, within PS417_10740 at 2295.363 kb on + strand, within PS417_10740 at 2295.364 kb on - strand at 2295.364 kb on - strand, within PS417_10740 at 2295.364 kb on - strand, within PS417_10740 at 2295.364 kb on - strand, within PS417_10740 at 2295.364 kb on - strand, within PS417_10740 at 2295.364 kb on - strand, within PS417_10740 at 2295.387 kb on - strand at 2295.387 kb on - strand, within PS417_10740 at 2295.508 kb on + strand at 2295.508 kb on + strand at 2295.509 kb on - strand at 2295.509 kb on - strand at 2295.509 kb on - strand at 2295.593 kb on + strand, within PS417_10745 at 2295.593 kb on + strand, within PS417_10745 at 2295.593 kb on + strand, within PS417_10745 at 2295.594 kb on - strand, within PS417_10745 at 2295.642 kb on + strand, within PS417_10745 at 2295.642 kb on + strand, within PS417_10745 at 2295.642 kb on + strand, within PS417_10745 at 2295.713 kb on + strand, within PS417_10745 at 2295.713 kb on + strand, within PS417_10745 at 2295.750 kb on + strand, within PS417_10745 at 2295.750 kb on + strand, within PS417_10745 at 2295.751 kb on - strand, within PS417_10745 at 2295.835 kb on + strand, within PS417_10745 at 2295.835 kb on + strand, within PS417_10745 at 2295.836 kb on - strand at 2295.869 kb on + strand, within PS417_10745 at 2295.869 kb on + strand, within PS417_10745 at 2295.870 kb on - strand at 2296.092 kb on + strand, within PS417_10745 at 2296.092 kb on + strand, within PS417_10745 at 2296.092 kb on + strand, within PS417_10745 at 2296.093 kb on - strand, within PS417_10745 at 2296.093 kb on - strand, within PS417_10745 at 2296.093 kb on - strand, within PS417_10745 at 2296.184 kb on - strand at 2296.223 kb on + strand at 2296.321 kb on - strand at 2296.321 kb on - strand at 2296.364 kb on + strand, within PS417_10750 at 2296.364 kb on + strand, within PS417_10750 at 2296.364 kb on + strand, within PS417_10750 at 2296.364 kb on + strand, within PS417_10750 at 2296.365 kb on - strand, within PS417_10750 at 2296.408 kb on + strand, within PS417_10750
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 2,294,307 + PS417_10730 0.76 -0.6 2,294,307 + PS417_10730 0.76 -1.0 2,294,308 - PS417_10730 0.76 -0.6 2,294,542 + -0.3 2,294,542 + +2.5 2,294,542 + +2.0 2,294,542 + -0.4 2,294,542 + +0.5 2,294,571 - -2.7 2,294,657 + +0.6 2,294,658 - -0.3 2,294,741 + -0.7 2,294,742 - +1.5 2,294,742 - -0.1 2,294,757 - PS417_10735 0.11 +0.6 2,294,874 + PS417_10735 0.34 +0.2 2,294,874 + PS417_10735 0.34 +0.9 2,294,898 - PS417_10735 0.38 +0.2 2,294,901 + PS417_10735 0.39 +0.2 2,294,902 - PS417_10735 0.39 +0.9 2,295,081 - PS417_10735 0.73 +1.6 2,295,085 - PS417_10735 0.73 -0.6 2,295,096 - PS417_10735 0.76 +0.0 2,295,223 - +0.3 2,295,293 + +0.0 2,295,294 - -0.1 2,295,357 + PS417_10740 0.27 -1.8 2,295,363 + PS417_10740 0.30 -0.4 2,295,364 - +1.0 2,295,364 - PS417_10740 0.30 -2.6 2,295,364 - PS417_10740 0.30 -0.3 2,295,364 - PS417_10740 0.30 -0.4 2,295,364 - PS417_10740 0.30 -1.0 2,295,364 - PS417_10740 0.30 +1.0 2,295,387 - -0.0 2,295,387 - PS417_10740 0.41 -4.0 2,295,508 + -2.4 2,295,508 + +0.1 2,295,509 - -0.1 2,295,509 - -3.5 2,295,509 - +0.8 2,295,593 + PS417_10745 0.11 +0.5 2,295,593 + PS417_10745 0.11 -0.7 2,295,593 + PS417_10745 0.11 -1.4 2,295,594 - PS417_10745 0.11 +0.2 2,295,642 + PS417_10745 0.18 +1.2 2,295,642 + PS417_10745 0.18 +0.1 2,295,642 + PS417_10745 0.18 -0.1 2,295,713 + PS417_10745 0.29 -0.3 2,295,713 + PS417_10745 0.29 -0.6 2,295,750 + PS417_10745 0.34 -2.1 2,295,750 + PS417_10745 0.34 -0.8 2,295,751 - PS417_10745 0.35 +0.0 2,295,835 + PS417_10745 0.47 -0.6 2,295,835 + PS417_10745 0.47 +0.4 2,295,836 - -0.0 2,295,869 + PS417_10745 0.52 -0.3 2,295,869 + PS417_10745 0.52 +0.3 2,295,870 - +2.0 2,296,092 + PS417_10745 0.85 -1.5 2,296,092 + PS417_10745 0.85 +1.0 2,296,092 + PS417_10745 0.85 -0.9 2,296,093 - PS417_10745 0.85 +0.2 2,296,093 - PS417_10745 0.85 +0.1 2,296,093 - PS417_10745 0.85 -1.7 2,296,184 - +0.9 2,296,223 + +0.2 2,296,321 - -1.0 2,296,321 - +0.1 2,296,364 + PS417_10750 0.14 -0.0 2,296,364 + PS417_10750 0.14 +0.7 2,296,364 + PS417_10750 0.14 +1.0 2,296,364 + PS417_10750 0.14 +0.1 2,296,365 - PS417_10750 0.14 +0.8 2,296,408 + PS417_10750 0.28 -0.0
Or see this region's nucleotide sequence