Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09000 and PS417_09005 are separated by 10 nucleotides PS417_09005 and PS417_09010 overlap by 4 nucleotides PS417_09010 and PS417_09015 are separated by 85 nucleotides
PS417_09000: PS417_09000 - glycerol-3-phosphate dehydrogenase, at 1,961,083 to 1,962,108
_09000
PS417_09005: PS417_09005 - lipase, at 1,962,119 to 1,962,466
_09005
PS417_09010: PS417_09010 - phosphohistidine phosphatase, at 1,962,463 to 1,962,912
_09010
PS417_09015: PS417_09015 - AMP-binding protein, at 1,962,998 to 1,964,647
_09015
Position (kb)
1961
1962
1963 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1960.118 kb on + strand at 1960.118 kb on + strand at 1960.118 kb on + strand at 1960.122 kb on + strand at 1960.122 kb on + strand at 1960.122 kb on + strand at 1960.125 kb on - strand at 1960.125 kb on - strand at 1960.610 kb on + strand at 1960.611 kb on - strand at 1960.883 kb on + strand at 1960.883 kb on + strand at 1960.883 kb on + strand at 1960.901 kb on + strand at 1960.901 kb on + strand at 1960.905 kb on + strand at 1960.924 kb on + strand at 1961.041 kb on - strand at 1961.082 kb on + strand at 1961.082 kb on + strand at 1962.146 kb on + strand at 1962.147 kb on - strand at 1962.249 kb on - strand, within PS417_09005 at 1962.614 kb on - strand, within PS417_09010 at 1962.656 kb on + strand, within PS417_09010 at 1962.656 kb on + strand, within PS417_09010 at 1962.657 kb on - strand, within PS417_09010 at 1962.937 kb on + strand at 1962.937 kb on + strand at 1962.937 kb on + strand at 1962.937 kb on + strand at 1962.937 kb on + strand at 1962.938 kb on - strand at 1962.938 kb on - strand at 1962.938 kb on - strand at 1962.938 kb on - strand at 1962.939 kb on + strand at 1962.939 kb on + strand at 1962.940 kb on - strand at 1962.940 kb on - strand at 1962.940 kb on - strand at 1962.940 kb on - strand at 1963.108 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,960,118 + +0.7 1,960,118 + -1.0 1,960,118 + +1.0 1,960,122 + +0.2 1,960,122 + -1.3 1,960,122 + +1.0 1,960,125 - -1.9 1,960,125 - -1.1 1,960,610 + -1.5 1,960,611 - -0.4 1,960,883 + -1.1 1,960,883 + +0.4 1,960,883 + -0.6 1,960,901 + -1.4 1,960,901 + -2.1 1,960,905 + -2.9 1,960,924 + +0.2 1,961,041 - -0.3 1,961,082 + -0.4 1,961,082 + -0.4 1,962,146 + +0.9 1,962,147 - +0.6 1,962,249 - PS417_09005 0.37 +1.0 1,962,614 - PS417_09010 0.34 -0.4 1,962,656 + PS417_09010 0.43 -2.0 1,962,656 + PS417_09010 0.43 -0.1 1,962,657 - PS417_09010 0.43 +0.1 1,962,937 + +0.0 1,962,937 + -1.8 1,962,937 + +0.0 1,962,937 + -0.2 1,962,937 + -1.0 1,962,938 - -0.4 1,962,938 - -0.0 1,962,938 - -0.4 1,962,938 - +0.5 1,962,939 + -2.7 1,962,939 + -1.0 1,962,940 - -0.2 1,962,940 - -0.4 1,962,940 - +0.3 1,962,940 - -1.2 1,963,108 + -0.6
Or see this region's nucleotide sequence