Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08310 and PS417_08315 are separated by 216 nucleotides PS417_08315 and PS417_08320 are separated by 44 nucleotides PS417_08320 and PS417_08325 are separated by 90 nucleotides
PS417_08310: PS417_08310 - alpha/beta hydrolase, at 1,820,746 to 1,821,783
_08310
PS417_08315: PS417_08315 - hypothetical protein, at 1,822,000 to 1,822,278
_08315
PS417_08320: PS417_08320 - metal-chelation protein CHAD, at 1,822,323 to 1,823,084
_08320
PS417_08325: PS417_08325 - acyl-CoA thioesterase, at 1,823,175 to 1,823,972
_08325
Position (kb)
1821
1822
1823 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1821.464 kb on + strand, within PS417_08310 at 1821.577 kb on + strand, within PS417_08310 at 1821.578 kb on - strand, within PS417_08310 at 1821.904 kb on - strand at 1821.904 kb on - strand at 1821.907 kb on + strand at 1821.907 kb on + strand at 1821.907 kb on + strand at 1821.907 kb on + strand at 1821.907 kb on + strand at 1821.907 kb on + strand at 1821.907 kb on + strand at 1821.908 kb on - strand at 1821.908 kb on - strand at 1821.908 kb on - strand at 1821.908 kb on - strand at 1821.908 kb on - strand at 1821.911 kb on + strand at 1821.911 kb on + strand at 1821.911 kb on + strand at 1821.912 kb on - strand at 1821.912 kb on - strand at 1821.912 kb on - strand at 1821.950 kb on + strand at 1821.951 kb on - strand at 1821.951 kb on - strand at 1821.951 kb on - strand at 1821.969 kb on - strand at 1822.019 kb on + strand at 1822.019 kb on + strand at 1822.154 kb on - strand, within PS417_08315 at 1822.158 kb on + strand, within PS417_08315 at 1822.158 kb on + strand, within PS417_08315 at 1822.249 kb on + strand at 1822.249 kb on + strand at 1822.249 kb on + strand at 1822.249 kb on + strand, within PS417_08315 at 1822.250 kb on - strand, within PS417_08315 at 1822.250 kb on - strand, within PS417_08315 at 1822.250 kb on - strand, within PS417_08315 at 1822.252 kb on - strand at 1822.372 kb on + strand at 1822.372 kb on + strand at 1822.373 kb on - strand at 1822.379 kb on - strand at 1822.573 kb on - strand, within PS417_08320 at 1822.883 kb on + strand, within PS417_08320 at 1822.883 kb on + strand, within PS417_08320 at 1822.883 kb on + strand, within PS417_08320 at 1823.108 kb on + strand at 1823.137 kb on + strand at 1823.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,821,464 + PS417_08310 0.69 +0.3 1,821,577 + PS417_08310 0.80 -0.2 1,821,578 - PS417_08310 0.80 -1.0 1,821,904 - +0.8 1,821,904 - -0.3 1,821,907 + -1.4 1,821,907 + +0.9 1,821,907 + +1.1 1,821,907 + -0.9 1,821,907 + -1.2 1,821,907 + -0.1 1,821,907 + -0.9 1,821,908 - +0.4 1,821,908 - +1.0 1,821,908 - -0.7 1,821,908 - +0.4 1,821,908 - -0.0 1,821,911 + +0.1 1,821,911 + +1.0 1,821,911 + -1.0 1,821,912 - +2.0 1,821,912 - +0.1 1,821,912 - +1.4 1,821,950 + -1.7 1,821,951 - +1.3 1,821,951 - +0.1 1,821,951 - -0.9 1,821,969 - +1.1 1,822,019 + -0.7 1,822,019 + +1.1 1,822,154 - PS417_08315 0.55 -0.4 1,822,158 + PS417_08315 0.57 +0.4 1,822,158 + PS417_08315 0.57 -0.2 1,822,249 + +1.4 1,822,249 + +2.1 1,822,249 + +2.1 1,822,249 + PS417_08315 0.89 +0.1 1,822,250 - PS417_08315 0.90 -1.3 1,822,250 - PS417_08315 0.90 +1.0 1,822,250 - PS417_08315 0.90 +1.3 1,822,252 - +3.9 1,822,372 + +1.8 1,822,372 + -1.4 1,822,373 - -0.8 1,822,379 - -0.2 1,822,573 - PS417_08320 0.33 +1.9 1,822,883 + PS417_08320 0.73 +0.5 1,822,883 + PS417_08320 0.73 +0.7 1,822,883 + PS417_08320 0.73 +0.2 1,823,108 + +3.4 1,823,137 + +0.2 1,823,183 + +0.5
Or see this region's nucleotide sequence